of resistance was noted against ceftazidime (47% to 77%) and ceftriaxone (43% to 71%). Imipenem (2% to 1%) and pefloxacin (40% to 17%) showed decreased trend. For Staphylococcus aureus, marked increase in resistance was shown against almost all antibiotics except co-trimoxazole (55% to 57%). Mentionable increase in resistance (p<0.05 and p<0.001, respectively) was noted against ciprofloxacin (17% to 43%) and ceftriaxone (28% to 83%). Although, oxacillin resistance increased from 22% to 42% but no resistance against vancomycin was noted during this period.Strains of Pseudomonas species showed increase (p<0.05) in resistance against ciprofloxacin (47% to 71%), ceftriaxone (50% to 74%) and ceftazidime (39% to 58%). Carbenicillin showed decreased resistance (92% to 50%) and none of the strains was found resistant to imipenem. Emphasis was given towards judicial use of antibiotics by followig local antibiogram.
This observational study was carried out as a preliminary one to see the effectiveness and reliability of an easy noninvasive approach for the confirmatory diagnosis of Visceral Leishmaniasis (VL) by detection of amastigote forms (LD bodies) of the parasite. Clinically suspected 73 febrile cases were investigated by examination of bone marrow/splenic aspirate to detect LD bodies, of whom 57 (41.61%) showed LD positive result on microscopy and/or culture in modified Novy, Nichole, MacNeal (NNN) medium. Blood buffy coat was obtained by the method of density gradient centrifugation using mono-nuclear cell separation fluid "Lymphoprep" from all the cases confirmed as VL. Thin smears on microscopic slides were prepared from all 57 buffy coats, and stained by Leishman stain to see LD bodies microscopically. Out of 57 buffy coat smears, 53 (92.98%) yielded LD bodies. Findings of the present study has encouraged to carry out a field trial so that this easy approach can be practiced as a clinical diagnostic procedure.Bangladesh J Med Microbiol 2007; 01 (02): 43-47
Helicobacter pylori is a bacterial infection of the stomach, which plays a major role in abdominal symptoms and gastroduodenal pathology. The pregnant women had a significantly higher relative risk of acquiring H.pylori infection during pregnancy as a result of physiological alterations. To investigate the relationship of H.pylori with dyspeptic symptoms in early and late pregnancy, thirty sera samples were obtained from pregnant women and thirty sera samples were obtained from apparently healthy women as control. All studied groups were evaluated anti-Helicobacter pylori IgG antibody by ELISA. Ten of pregnant women samples (33.3%) were seropositive of anti-H. pylori IgG antibody in pregnant women compared with control. This lead to suggest that H. pylori positive may be related to nausea and vomiting in pregnant women.
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