LC–MS Determination of Triazolam and its Hydroxy Metabolites in Mouse Dried Blood Spots: Application to Transgenic Mouse Pharmacokinetic Studies

Kole, Prashant; Rao, Abhijith; Kurawattimath, Vishwanath; Mandlekar, Sandhya

doi:10.4155/bio-2017-0067

Cited by 8 publications

(1 citation statement)

References 34 publications

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“…31,81 Recent advancements in instrumentation have achieved improved accuracy and sensitivity which has led to magnified attention in the use of microsamples. 42,46,[80][81][82] The range of applications include therapeutic drugs, [83][84][85][86] biomarkers, 87,88 proteins and peptides, 89 drugs of abuse, 90,91 hormones and steroids, 92,93 DNA/RNA 56,87 and cytokines. 27,53,94 Few established applications discussed below focus on the use of microsampling techniques in the determination of drug concentration using a validated bioanalytical method.…”

Section: Applications Of Microsampling In the Development Of Treatmentsmentioning

confidence: 99%

Microsampling: A role to play in Covid‐19 diagnosis, surveillance, treatment and clinical trials

Rajadhyaksha

Londhe

2021

Drug Testing and Analysis

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The outbreak of the new coronavirus disease changed the world upside down. Every day, millions of people were subjected to diagnostic testing for Covid‐19, all over the world. Molecular tests helped in the diagnosis of current infection by detecting the presence of viral genome whereas serological tests helped in detecting the presence of antibody in blood as well as contributed to vaccine development. This testing helped in understanding the immunogenicity, community prevalence, geographical spread and conditions post‐infection. However, with the contagious nature of the virus, biological specimen sampling involved the risk of transmission and spread of infection. Clinic or pathology visit was the most concerning part. Trained personnel and resources was another barrier. In this scenario, microsampling played an important role due to its most important advantage of remote, contactless, small volume and self‐sampling. Minimum requirements for sample storage and ease of shipment added value in this situation. The highly sensitive instruments and validated assay formats assured the accuracy of results and stability of samples. Microsampling techniques are contributing effectively to the Covid‐19 pandemic by reducing the demand for clinical staff in population‐level testing. The validated and established applications supported the use of microsampling in diagnosis, therapeutic drug monitoring, development of treatment or vaccines and clinical trials for Covid‐19.

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Section: Applications Of Microsampling In the Development Of Treatmentsmentioning

confidence: 99%

Microsampling: A role to play in Covid‐19 diagnosis, surveillance, treatment and clinical trials

Rajadhyaksha

Londhe

2021

Drug Testing and Analysis

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Opportunities and obstacles for microsampling techniques in bioanalysis: Special focus on DBS and VAMS

Londhe

Rajadhyaksha

2020

Journal of Pharmaceutical and Biomedical Analysis

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Pharmacokinetics, oral bioavailability and metabolic analysis of solasodine in mice by dried blood spot LC-MS/MS and UHPLC-Q-Exactive MS

Peng

Zhang

et al. 2022

Journal of Pharmaceutical and Biomedical Analysis

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LC–MS Determination of Triazolam and its Hydroxy Metabolites in Mouse Dried Blood Spots: Application to Transgenic Mouse Pharmacokinetic Studies

Abstract: This validated LC-MS/MS method using DBS extraction was applied to quantitation of TRZ, α-hydroxytriazolam and 4-hydroxytriazolam in a CYP3A4 transgenic mouse oral pharmacokinetic study of TRZ.

Cited by 8 publications

References 34 publications

Microsampling: A role to play in Covid‐19 diagnosis, surveillance, treatment and clinical trials

Microsampling: A role to play in Covid‐19 diagnosis, surveillance, treatment and clinical trials

Opportunities and obstacles for microsampling techniques in bioanalysis: Special focus on DBS and VAMS

Pharmacokinetics, oral bioavailability and metabolic analysis of solasodine in mice by dried blood spot LC-MS/MS and UHPLC-Q-Exactive MS

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