Pharmacokinetics, oral bioavailability and metabolic analysis of solasodine in mice by dried blood spot LC-MS/MS and UHPLC-Q-Exactive MS

Qu, Mei; Peng, Xue; Zhang, Qi; Lu, Tiantian; Liu, Kun; Hu, Binwu; Jing-jing, Pang; Xiao, Qianqian; Xu, Tongxin; Wang, Quan‐De; Cheng, Zhongzhe

doi:10.1016/j.jpba.2021.114542

Cited by 8 publications

(15 citation statements)

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“…Furthermore, solasodine demonstrates promotion of neurogenesis, neuroprotection and anticonvulsant activities in vivo [6][7][8]. The pharmacokinetics, oral bioavailability and metabolic profiling of solasodine in mice were evaluated in our earlier work [9]. A poor bioavailability (1.28%) was observed in mice following oral administration [9].…”

Section: Introductionmentioning

confidence: 99%

“…The pharmacokinetics, oral bioavailability and metabolic profiling of solasodine in mice were evaluated in our earlier work [9]. A poor bioavailability (1.28%) was observed in mice following oral administration [9]. Meanwhile, solasodine could be metabolized into 21 metabolites, indicating that solasodine was subjected to an extensive metabolism in vivo [9].…”

Section: Introductionmentioning

confidence: 99%

“…A poor bioavailability (1.28%) was observed in mice following oral administration [9]. Meanwhile, solasodine could be metabolized into 21 metabolites, indicating that solasodine was subjected to an extensive metabolism in vivo [9]. Moreover, the excretion of solasodine were investigated by injection of [ 3 H]-solasodine [10].…”

Section: Introductionmentioning

confidence: 99%

“…Previously, the quantification of solasodine in rat plasma and mouse blood using liquid chromatography-tandem mass spectrometry (LC-MS/MS) method were developed, respectively [9,11]. However, there is little bioanalytical method for the determinatin of solasodine in urinary and fecal samples.…”

Section: Introductionmentioning

confidence: 99%

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Validated LC-MS/MS method for quantitation of solasodine in rat urine and feces: Blocking nonspecific adsorption

Wang

Zhang

et al. 2023

AChrom

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Solasodine, a steroidal alkaloid, is distributed extensively in Solanaceae plants with multiple biological activities such as neuroprotection, antineoplastic and anticonvulsant activities. However, there is little information about the excretion of intact solasodine in vivo. To investigate its excretion, a reliable LC-MS/MS method for quantitation solasodine in rat urine and feces was established and validated. Sample preparation was carried out by liquid-liquid extraction using MTBE as extractant. Moreover, rat urine was preconditioned with BSA, an anti-adsorptive additive, to prevent the nonspecific binding of solasodine to containers and tubes. The method was validated over the range of 4–2000 ng mL−1. The correlation coefficient (r 2) were all above 0.999. The intra- and inter-day precision and accuracy were within 16.9% and between −11.0 and 8.9%, respectively. The recovery of solasodine in urine and feces was in the range of 72.5–80.3 and 75.7–80.2%, respectively. IS-normalized matrix factor ranged from 0.94 to 1.12 with RSD% ≤4.02%. This method was successfully applied to the excretion study of solasodine following oral and intravenous administration.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Validated LC-MS/MS method for quantitation of solasodine in rat urine and feces: Blocking nonspecific adsorption

Wang

Zhang

et al. 2023

AChrom

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“…In recent years, because of the development of high resolution and high sensitivity of the mass spectrometry technology, liquid chromatography coupled with mass spectrometer systems have been used as effective and reliable analytical instruments to identify metabolites. , The Thermo Q Exactive mass spectrometer combines a high-performance quadrupole for ion selectivity and Orbitrap detection technology for high-resolution determination of accurate molecular masses, providing both qualitative and quantitative detection function. − The system resolution can reach 140,000 full width at half maximum (FWHM) in full scan mode. Correspondingly, specific software, including Compound Discoverer 3.2, TraceFinder 4.1, Xcalibur 3.1.66, and Mass Frontier 7.0 can comprehensively trace and screen the probable generated metabolites in complex biological matrices with multiple data-processing techniques, such as the mass defect filter (MDF), extract ion chromatogram (XIC), neutral loss filter (NLF), isotope pattern filter (IPF), product ion filter (PIF), and dynamic background subtraction (DBS).…”

Section: Introductionmentioning

confidence: 99%

A Systematic Strategy for the Characterization of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-d-glucoside Metabolites In Vivo by Ultrahigh Performance Liquid Chromatography Coupled with a Q Exactive-Orbitrap Mass System

Huang

Zhang

et al. 2022

J. Agric. Food Chem.

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Tetrahydroxystilbene-2-O-β-D-glucoside (THSG), a polyphenol stilbene compound, is the main active constituent in Polygonum multiflorum. In this study, a comprehensive analytical strategy was developed for the characterization of THSG metabolites in vivo (rat plasma, bile, urine, heart, liver, spleen, lung, kidney, and stomach) utilizing ultrahigh performance liquid chromatography coupled with Q Exactive hybrid quadrupole-Orbitrap mass spectrometry (UHPLC-Q Exactive-Orbitrap MS) based on multiple data-processing techniques. As a result, a total of 75 metabolites were characterized in bio-samples, and calculated Clog P values were further employed to assign the chemical structures of some isomers. Glucoside hydrolysis, hydrogenation, hydroxylation, glucuronide conjugation, and sulfate conjugation would be the major metabolic pathways of THSG. It appeared that most metabolites would generally undergo phase I reactions followed by phase II reactions. These results provided valuable information for in-depth understanding of the safety and efficacy of THSG and showed a valuable methodology for metabolic characterization.

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Bioactivities and chemical profiling comparison and metabolomic variations of polyphenolics and steroidal glycoalkaloids in different parts of Solanum nigrum L.

Zhang,

Liu,

Cui

et al. 2023

Phytochemical Analysis

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IntroductionSolanum nigrum L. is a traditional medicinal herb and edible plant. Many studies provide evidence that S. nigrum L. is a nutritious vegetable. Polyphenols and steroidal glycoalkaloids are the main components.ObjectivesThis study aimed to systemically evaluate the phytochemical profile, quantification, and bioactivities of polyphenolics and glycoalkaloids in different parts of S. nigrum L.ResultsTotal polyphenols (TPC) and total glycoalkaloids (TGK) were determined using the Folin–Ciocalteu and acid dye colorimetric methods, respectively. A total of 55 polyphenolic constituents (including 22 phenolic acids and 33 flavonoids) and 24 steroidal glycoalkaloids were identified from different parts using ultrahigh‐performance liquid chromatography Q‐exactive high‐resolution mass spectrometry (UHPLC‐QE‐HRMS), of which 40 polyphenols (including 15 phenolic acids and 25 flavonoids) and one steroidal glycoalkaloid were characterised for the first time in S. nigrum L. Moreover, typical polyphenols and glycoalkaloids were determined using HPLC‐UV and HPLC–evaporative light‐scattering detector (ELSD), respectively. In addition, the TPC and TGK and their typical constituents were compared in different anatomical parts. Finally, the antioxidant capacities of polyphenolic extracts from different parts of S. nigrum L. were evaluated by ·OH, 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging and ferric‐reducing antioxidant power (FRAP) assay in vitro. In addition, the antitumour effects of TGK from different parts of S. nigrum L. on the proliferation of PC‐3 cells were investigated using 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay. Polyphenolic and glycoalkaloid extracts from different parts of S. nigrum L. showed different antioxidant and cytotoxic capacities in vitro.ConclusionThis is the first study to systematically differentiate between polyphenolic and glycoalkaloid profiles from different parts of S. nigrum L.

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Pharmacokinetics, oral bioavailability and metabolic analysis of solasodine in mice by dried blood spot LC-MS/MS and UHPLC-Q-Exactive MS

Cited by 8 publications

References 24 publications

Validated LC-MS/MS method for quantitation of solasodine in rat urine and feces: Blocking nonspecific adsorption

Validated LC-MS/MS method for quantitation of solasodine in rat urine and feces: Blocking nonspecific adsorption

A Systematic Strategy for the Characterization of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-d-glucoside Metabolites In Vivo by Ultrahigh Performance Liquid Chromatography Coupled with a Q Exactive-Orbitrap Mass System

Bioactivities and chemical profiling comparison and metabolomic variations of polyphenolics and steroidal glycoalkaloids in different parts of Solanum nigrum L.

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