Laser-induced vapor nanobubbles improve diffusion in biofilms of antimicrobial agents for wound care

Teirlinck, Eline; Fraire, Juan C.; Acker, Heleen Van; Wille, Jasper; Swimberghe, R. C. D.; Brans, Toon; Xiong, Ranhua; Meire, Maarten; Moor, Roeland De; Smedt, Stefaan C. De; Coenye, Tom; Braeckmans, Kevin

doi:10.1016/j.bioflm.2019.100004

Cited by 21 publications

(19 citation statements)

References 63 publications

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“…The organized structure of the biofilm was compromised by the generated pressure waves, enabling a better diffusion of the drug molecules deep into the biofilm. In fact, the treatment with vapor nanobubbles enhanced the antibacterial effects of the tested compounds, achieving results comparable with the ones obtained for forced disrupted biofilms, by sonication and vortexing (Teirlinck et al, 2019).…”

Section: Photodynamic Therapy and Ultrasoundssupporting

confidence: 75%

“…Besides ultrasounds, laser light can also be used to induce the vaporization of nanobubbles, producing similar damage on the matrix and potentiating antibiotic effects (Teirlinck et al, 2019). Teirlinck et al tested this approach against P. aeruginosa and S. aureus biofilms, in conjugation with small gold NPs.…”

Section: Photodynamic Therapy and Ultrasoundsmentioning

confidence: 99%

“…Teirlinck et al tested this approach against P. aeruginosa and S. aureus biofilms, in conjugation with small gold NPs. When short laser pulses were applied to the biofilms, these NPs could absorb the energy of high intensity, heating the water surrounding of the particles that quickly evaporated, resulting in expanding and imploding water vapor nanobubbles (Teirlinck et al, 2019). The organized structure of the biofilm was compromised by the generated pressure waves, enabling a better diffusion of the drug molecules deep into the biofilm.…”

Section: Photodynamic Therapy and Ultrasoundsmentioning

confidence: 99%

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Innovative Strategies Toward the Disassembly of the EPS Matrix in Bacterial Biofilms

et al. 2020

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Bacterial biofilms represent a major concern at a worldwide level due to the high demand for implantable medical devices and the rising numbers of bacterial resistance. The complex structure of the extracellular polymeric substances (EPS) matrix plays a major role in this phenomenon, since it protects bacteria from antibiotics, avoiding drug penetration at bactericidal concentrations. Besides, this structure promotes bacterial cells to adopt a dormant lifestyle, becoming less susceptible to antibacterial agents. Currently, the available treatment for biofilm-related infections consists in the administration of conventional antibiotics at high doses for a long-term period. However, this treatment lacks efficiency against mature biofilms and for implant-associated biofilms it may be necessary to remove the medical device. Thus, biofilm-related infections represent an economical burden for the healthcare systems. New strategies focusing on the matrix are being highlighted as alternative therapies to eradicate biofilms. Here, we outline reported matrix disruptive agents, nanocarriers, and technologies, such as application of magnetic fields, photodynamic therapy, and ultrasounds, that have been under investigation to disrupt the EPS matrix of clinically relevant bacterial biofilms. In an ideal therapy, a synergistic effect between antibiotics and the explored innovated strategies is aimed to completely eradicate biofilms and avoid antimicrobial resistance phenomena.

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Section: Photodynamic Therapy and Ultrasoundssupporting

confidence: 75%

Section: Photodynamic Therapy and Ultrasoundsmentioning

confidence: 99%

Section: Photodynamic Therapy and Ultrasoundsmentioning

confidence: 99%

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Innovative Strategies Toward the Disassembly of the EPS Matrix in Bacterial Biofilms

et al. 2020

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“…Only few studies showed antimicrobial and antibiofilm properties of nanobubbles in combination with chemical sanitizers [ 16 , 17 , 21 , 25 , 26 ]. In our previous study, we showed antibiofilm properties on nanobubbles alone and in combination with chemical sanitizers [ 21 ].…”

Section: Resultsmentioning

confidence: 99%

“…Cavitative collapse of nanobubbles generates reactive oxygen species, as well as a physical insult to microbial cell structures [ 15 , 16 , 17 , 18 ]. Due to nanobubbles’ unique properties, this technology has been applied in various areas of advanced science and technology including engineering, medical, agricultural, and food sectors [ 16 , 17 , 18 , 19 , 20 , 21 ]; for cleaning surfaces [ 22 , 23 , 24 ]; dental hygiene [ 25 ]; wound cleaning [ 16 , 17 ]; removing bacteria from fresh produce [ 26 ]; inactivation of norovirus [ 27 ]; and removing microbial biofilms [ 21 ]. Nanobubbles can exist in both bulk solution and at liquid–solid interfaces, and due to their unique physical properties (nanosize diameter, negative surface charge and Brownian motion), nanobubbles can remain stable for up to 24 h, resulting in a supersaturated bubble phase [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Inactivation of Aeromonas hydrophila and Vibrio parahaemolyticus by Curcumin-Mediated Photosensitization and Nanobubble-Ultrasonication Approaches

Rafeeq

Shiroodi

Schwarz

et al. 2020

Foods

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The antimicrobial efficacy of novel photodynamic inactivation and nanobubble technologies was evaluated against Vibrio parahaemolyticus and Aeromonas hydrophila as two important aquatic microbial pathogens. Photodynamic inactivation results showed that LED (470 nm) and UV-A (400 nm)-activated curcumin caused a complete reduction in V. parahaemolyticus at 4 and 22 °C, and a greater than 2 log cfu/mL reduction in A. hydrophila, which was curcumin concentration-dependent (p < 0.05). Furthermore, the photodynamic approach caused a greater than 6 log cfu/mL V. parahaemolyticus reduction and more than 4 log cfu/mL of A. hydrophila reduction in aquaponic water samples (p < 0.05). Our results with the nanobubble technology showed that the nanobubbles alone did not significantly reduce bacteria (p > 0.05). However, a greater than 6 log cfu/mL A. hydrophila reduction and a greater than 3 log cfu/mL of V. parahaemolyticus reduction were achieved when nanobubble technology was combined with ultrasound (p < 0.05). The findings described in this study illustrate the potential of applying photodynamic inactivation and nanobubble–ultrasound antimicrobial approaches as alternative novel methods for inactivating fish and shellfish pathogens.

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Guanidine and Galactose Decorated Nanophotosensitizer with Oxygen Self‐Sufficient Capability for the Localized Ablation of Oral Biofilm

Wang

Chen

et al. 2023

Adv Funct Materials

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Dental caries is a common disease caused by plaque biofilms, which are important pathogenic factors in many diseases. When hosts are overexposed to dietary sugars, pathogens such as Streptococcus mutans (S. mutans) and other cariogenic bacteria, metabolically assemble an extracellular matrix rich in exopolysaccharides to form a disease‐causing biofilm, in which the microenvironment is characterized by regional hypoxia, low pH, and nutritional deprivation. Current antimicrobials with inadequate penetration and a lack of pathogens targeting the biofilm do not degrade the protective matrix within the biofilm. In this study, a guanidine and galactose decorated nanophotosensitizer with oxygen self‐sufficient capability, p(GF/GEF)‐I, is developed to enhance the permeability of biofilms by positively charging the particle surface and easily binding to the bacteria within the membrane through electrostatic interactions. 90% of the biofilm on enamel surface is eliminated after treatment with p(GF/GEF)‐I under laser irradiation. Notably, the nanophotosensitizer inhibits the recolonization of dental biofilms by S. mutans, preventing secondary infections. Furthermore, dental caries in a rodent model are reduced with exposure to nanophotosensitizer. p(GF/GEF)‐I is a significantly higher efficacy without damaging the surrounding soft tissue. With further development and optimization, p(GF/GEF)‐I shows significant potential as a phototherapeutic agent for the treatment of biofilm‐induced diseases.

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Laser-induced vapor nanobubbles improve diffusion in biofilms of antimicrobial agents for wound care

Cited by 21 publications

References 63 publications

Innovative Strategies Toward the Disassembly of the EPS Matrix in Bacterial Biofilms

Innovative Strategies Toward the Disassembly of the EPS Matrix in Bacterial Biofilms

Inactivation of Aeromonas hydrophila and Vibrio parahaemolyticus by Curcumin-Mediated Photosensitization and Nanobubble-Ultrasonication Approaches

Guanidine and Galactose Decorated Nanophotosensitizer with Oxygen Self‐Sufficient Capability for the Localized Ablation of Oral Biofilm

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