Laser Enhanced Combinatorial Chemo-photothermal Therapy of Green Synthesis Gold Nanoparticles Loaded with 6Mercaptopurine on Breast Cancer Model

Faid, Amna H.; Shouman, Samia A.; Thabet, Nadia A.; Badr, Y.; Sliem, Mahmoud A.

doi:10.1007/s12247-022-09626-0

Cited by 17 publications

(8 citation statements)

References 27 publications

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“…Moreover, the better electrostatic attraction of DOX with AuNPs than citrate groups might be accountable for the extra band at longer wavelengths. It has been revealed theoretically and experimentally that aggregation of AuNPs leads to additional plasmon absorption at higher wavelengths when nanoparticles are electronically attached to each other [ 16 ]. Consequently, violet color of the DOX-AuNPs solution as well as the UV-Vis spectrum slightly unchanged after 72 h storage in DMEM at room temperature, indicating the formation of a stable particle suspension, as shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Enhanced cytotoxic effect of doxorubicin conjugated gold nanoparticles on breast cancer model

et al. 2022

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Background The difficulty of achieving targeted drug delivery following administration of presently marketed anticancer therapeutics is still a concern. Metallic nanoparticles (NPs) appear to be promising in this regard. The present study focused on the use of gold nanoparticles (AuNPs) as a drug carrier for anticancer Doxorubicin (DOX) forming DOX–AuNPs nanocomposite. The anticancer effect of the prepared nanocomposite was evaluated using SRP essay on breast cancer cell line (MCF7) for different incubation times (24 h,48, and72hr). The prepared DOX–AuNPs nanocomposite was investigated by UV–visible spectroscopy, TEM, fluorescence spectroscopy, and FTIR spectroscopy. Results Our results showed that the prepared AuNPs and DOX–AuNPs nanocomposite have spherical and small size10 ± 2 nm and 12 ± 2 nm respectively. The potential cytotoxicity of the DOX-AuNPs nanocomposite on the MCF7 cell line was significantly increased compared to free DOX. The 20 µM DOX- AuNPs nanocomposite produced a similar decrease in cell survival as 80 µM free DOX. Conclusion Future work is in progress to investigate the positive effects of the prepared nanocomposite for chemo-photothermal combination treatment.

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Section: Resultsmentioning

confidence: 99%

Enhanced cytotoxic effect of doxorubicin conjugated gold nanoparticles on breast cancer model

et al. 2022

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“…Generally, nanocarriers are non-specifically internalized into cells via endocytosis or phagocytosis compared to the passive diffusion mechanism of free drugs into the cell [ 65 ]. Positively charged nanocarriers like 6MP-CNPs and 6MP-CNPs-AuNPs were known to facilitate their penetration through the negatively charged cancer cell membrane [ 66 ]. So, the electrostatic interaction could enhance the absorption of our proposed nanocarriers by MCF7cells.…”

Section: Discussionmentioning

confidence: 99%

Gold nanoparticles loaded chitosan encapsulate 6-mercaptopurine as a novel nanocomposite for chemo-photothermal therapy on breast cancer

Faid

Shouman²,

Badr

et al. 2022

BMC Chemistry

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Background As a promising strategy to overcome the therapeutic disadvantages of 6-mercaptopurine (6MP), we proposed the encapsulation of 6MP in chitosan nanoparticles (CNPs) to form the 6MP-CNPs complexes. The encapsulation was followed by the loading of complexes on gold nanoparticles (AuNPs) to generate a novel 6MP-CNPs-AuNPs nanocomposite to facilitate the chemo-photothermal therapeutic effect. Methods CNPs were produced based on the ionic gelation method of tripolyphosphate (TPP). Moreover, 6MP-CNPs composite were prepared by the modified ionic gelation method and then loaded on AuNPs which were synthesized according to the standard wet chemical method using trisodium citrate as a reducing and capping agent. The synthesized nanocomposites were characterized by UV–VIS spectroscopy, dynamic light scattering, Fourier transform infrared spectroscopy, and transmission electron microscopy. The potential cytotoxicity of the prepared nanocomposites on MCF7 cell line was carried out using Sulphorhodamine-B (SRB) assay. Results Optimization of CNPs, 6MP-CNPs, and 6MP-CNPs-AuNPs revealed 130 ± 10, 200 ± 20, and 25 ± 5 nm particle size diameters with narrow size distributions and exhibited high stability with zeta potential 36.9 ± 4.11, 37, and 44.4 mV, respectively. The encapsulation efficiency of 6MP was found to be 57%. The cytotoxicity of 6MP-CNPs and 6MP-CNPs-AuNPs on breast cell line MCF7 was significantly increased and reached IC50 of 9.3 and 8.7 µM, respectively. The co-therapeutic effect of the nanocomposites resulted in an improvement of the therapeutic efficacy compared to the individual effect of chemo- and photothermal therapy. Irradiation of 6MP-CNPs and 6MP-CNPs-AuNPs with a diode laser (DPSS laser, 532 nm) was found to have more inhibition on cell viability with a decrease in IC50 to 5 and 4.4 µM, respectively. Conclusion The Chemo-Photothermal co-therapy treatment with novel prepared nanocomposite exhibits maximum therapeutic efficacy and limits the dosage-related side effects of 6MP. Graphical Abstract

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“…The color of the AuNPs' suspension was dark purple. The narrow band of the curve corresponds mostly to the monodisperse nature of AuNPs, without any aggregation and agglomeration [20,21]. TEM observations were employed to clarify the morphology of formed AuNPs; the particles are spherical with approximate size (12 ± 2 nm) with uniform size distribution.…”

Section: Size Zeta Potential and Morphological Analysismentioning

confidence: 99%

Utilization of gold nanoparticles for the detection of squamous cell carcinoma of the tongue based on laser-induced fluorescence and diffuse reflectance characteristics: an in vitro study

et al. 2022

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Squamous cell carcinoma is a very common type of oral cancer that affects the health of people with an unacceptably high mortality rate attributed to the difficulties in detecting the disease at an early stage. Therefore, effective techniques for early diagnosis and effective therapy of oral cancer are necessary. In the present study, we exploit the ability of gold nanoparticles (AuNPs) to undergo coupled surface plasmon resonance when closely spaced to improve diagnosing squamous cell carcinoma of the tongue. The prepared AuNPs are characterized by UV–VIS spectroscopy, dynamic light scattering, Fourier transform infrared spectroscopy, and transmission electron microscopy. The size of the prepared AuNPs is 12 ± 2 nm with narrow size distributions and exhibited high stability with a zeta potential of − 16.5 mV. The light fluorescence of the normal and cancer cells is recorded before and after NP addition using a spectrometer upon excitation by 405-nm laser irradiation. Furthermore, the light reflectance of the examined samples is measured at different laser wavelengths (red to NIR region). The obtained results show that the cancer cells mixed with AuNPs produce a higher fluorescence peak at 489.2 nm than the cancer cells without AuNPs. Moreover, the optical diffuse reflectance analyses reveal that the addition of AuNPs enhances cancer detection especially at the 635-nm irradiation with sensitivity (94%), specificity (87%), and overall accuracy (91%).

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Laser Enhanced Combinatorial Chemo-photothermal Therapy of Green Synthesis Gold Nanoparticles Loaded with 6Mercaptopurine on Breast Cancer Model

Cited by 17 publications

References 27 publications

Enhanced cytotoxic effect of doxorubicin conjugated gold nanoparticles on breast cancer model

Enhanced cytotoxic effect of doxorubicin conjugated gold nanoparticles on breast cancer model

Gold nanoparticles loaded chitosan encapsulate 6-mercaptopurine as a novel nanocomposite for chemo-photothermal therapy on breast cancer

Utilization of gold nanoparticles for the detection of squamous cell carcinoma of the tongue based on laser-induced fluorescence and diffuse reflectance characteristics: an in vitro study

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