Abstract:Background
The difficulty of achieving targeted drug delivery following administration of presently marketed anticancer therapeutics is still a concern. Metallic nanoparticles (NPs) appear to be promising in this regard. The present study focused on the use of gold nanoparticles (AuNPs) as a drug carrier for anticancer Doxorubicin (DOX) forming DOX–AuNPs nanocomposite. The anticancer effect of the prepared nanocomposite was evaluated using SRP essay on breast cancer cell line (MCF7) for differe… Show more
“…In addition, there were slightly blue shifts for C–N stretching to1632 cm −1 . The free –NH group may be involved in the binding of Cis on the CoAg confirming the successful conjugation of Cisplatin on CoAg nanohybrid [ 30 ]. Fig.…”
Section: Resultsmentioning
confidence: 96%
“…Due to their potential use in numerous biomedical applications, metallic nanoparticles' distinctive optical, electrical, and biological characteristics have drawn substantial attention [ 27 – 29 ]. controlled release, drug targeting, and much higher bioavailability of medications are all features of nanodrug delivery systems which greatly overcome the weaknesses of traditional drug delivery [ 30 – 34 ]. According to earlier research, poly-nano-complexes and anticancer medications can boost the anticancer drug accumulation in tumor cells for a more effective treatment outcome [ 35 – 37 ].…”
Cancer is a deadly illness with a convoluted pathogenesis. The most prevalent restrictions that frequently result in treatment failure for cancer chemotherapy include lack of selectivity, cytotoxicity, and multidrug resistance. Thus, considerable efforts have been focused in recent years on the establishment of a modernistic sector termed nano-oncology, which offers the option of employing nanoparticles (NPs) with the objective of detecting, targeting, and treating malignant disorders. NPs offer a focused approach compared to conventional anticancer methods, preventing negative side effects. In the present work, a successful synthetic process was used to create magnetic cobalt cores with an AgNPs shell to form bimetallic nanocomposites CoAg, then functionalized with Cis forming novel CoAg@Cis nanohybrid. The morphology and optical properties were determined by TEM, DLS, FTIRs and UV–vis spectroscopy, furthermore, anticancer effect of CoAg and CoAg@Cis nanohybrids were estimated using MTT assay on MCF7 and HCT cell lines. Our results showed that Co@Ag core shell is about 15 nm were formed with dark CoNPs core and AgNPs shell with less darkness than the core, moreover, CoAg@Cis has diameter about 25 nm which are bigger in size than Co@Ag core shell demonstrating the loading of Cis. It was observed that Cis, CoAg and CoAg@Cis induced a decline in cell survival and peaked at around 65%, 73%and 66% on MCF7 and 80%, 76%and 78% on HCT at 100 µg/ml respectively. Compared to Cis alone, CoAg and CoAg@Cis caused a significant decrease in cell viability. These findings suggest that the synthesized CoAg can be used as a powerful anticancer drug carrier.
Graphical Abstract
“…In addition, there were slightly blue shifts for C–N stretching to1632 cm −1 . The free –NH group may be involved in the binding of Cis on the CoAg confirming the successful conjugation of Cisplatin on CoAg nanohybrid [ 30 ]. Fig.…”
Section: Resultsmentioning
confidence: 96%
“…Due to their potential use in numerous biomedical applications, metallic nanoparticles' distinctive optical, electrical, and biological characteristics have drawn substantial attention [ 27 – 29 ]. controlled release, drug targeting, and much higher bioavailability of medications are all features of nanodrug delivery systems which greatly overcome the weaknesses of traditional drug delivery [ 30 – 34 ]. According to earlier research, poly-nano-complexes and anticancer medications can boost the anticancer drug accumulation in tumor cells for a more effective treatment outcome [ 35 – 37 ].…”
Cancer is a deadly illness with a convoluted pathogenesis. The most prevalent restrictions that frequently result in treatment failure for cancer chemotherapy include lack of selectivity, cytotoxicity, and multidrug resistance. Thus, considerable efforts have been focused in recent years on the establishment of a modernistic sector termed nano-oncology, which offers the option of employing nanoparticles (NPs) with the objective of detecting, targeting, and treating malignant disorders. NPs offer a focused approach compared to conventional anticancer methods, preventing negative side effects. In the present work, a successful synthetic process was used to create magnetic cobalt cores with an AgNPs shell to form bimetallic nanocomposites CoAg, then functionalized with Cis forming novel CoAg@Cis nanohybrid. The morphology and optical properties were determined by TEM, DLS, FTIRs and UV–vis spectroscopy, furthermore, anticancer effect of CoAg and CoAg@Cis nanohybrids were estimated using MTT assay on MCF7 and HCT cell lines. Our results showed that Co@Ag core shell is about 15 nm were formed with dark CoNPs core and AgNPs shell with less darkness than the core, moreover, CoAg@Cis has diameter about 25 nm which are bigger in size than Co@Ag core shell demonstrating the loading of Cis. It was observed that Cis, CoAg and CoAg@Cis induced a decline in cell survival and peaked at around 65%, 73%and 66% on MCF7 and 80%, 76%and 78% on HCT at 100 µg/ml respectively. Compared to Cis alone, CoAg and CoAg@Cis caused a significant decrease in cell viability. These findings suggest that the synthesized CoAg can be used as a powerful anticancer drug carrier.
Graphical Abstract
“…DOX entrapped DMAB-AuNBP particles showed significantly higher anticancer drug (DOX) response for substantial cancer cell death (∼84%) in elevated acidic conditions (pH 2–4 for cancer cell interior) with quick uptake . When illuminated with NIR light to activate photothermal heating, DOX-DMAB-AuNBP particles showed even higher cancer cell killing (more than 90%) due to synergistic photochemotherapeutics response, viz., photo triggered release of DOX. , Prior to cancer therapeutics ability of plasmonic nanoparticles, the photothermal profile of DMAB-AuNBPs at four different concentrations was separately evaluated in aqueous solution after irradiation with an 808 nm laser (2 W/cm 2 , SI, Figure S4a). Phototransduction response of DMAB-AuNBPs also measured at various power densities (0.5–2.0 W/cm 2 ) as shown in SI, Figure S4b in the Supporting Information.…”
Surface-engineered gold nanoparticles have been considered as versatile systems for theranostics applications. Moreover, surface covering or stabilizing agents on gold nanoparticles especially gold nanobipyramids (AuNBPs) provides an extra space for cargo molecules entrapment. However, it is not well studied yet and also the preparation of AuNBPs still remains dependent largely on cetyltrimethylammonium bromide (CTAB), a cytotoxic surfactant. Therefore, the direct use of CTAB stabilized nanoparticles is not recommended for cancer theranostics applications. Herein, we address an approach of dodecyl ethyl dimethylammonium bromide (DMAB) as biocompatible structure directing agent for AuNBPs, which also accommodate anticancer drug doxorubicin (45%), an additional chemotherapeutics agent. Upon nearinfrared light (NIR, 808 nm) exposure, engineered AuNBPs exhibit (i) better phototransduction (51 °C) due to NIR absorption ability (650−900 nm), (ii) photo triggered drug release (more than 80%), and (iii) synergistic chemophototherapy for breast cancer cells. Drug release response has been evaluated in tumor microenvironment conditions (84% in acidic pH and 80% at high GSH) due to protonation and high affinity of thiol binding with AuNBPs followed by DMAB replacement. Intracellular glutathione (GSH, 5−7.5 mM) replaces DMAB from AuNBPs, which cause easy aggregation of nanoparticles as corroborated by colorimetric shifts, suggesting their utilization as a molecular sensing probe of early stage cancer biomarkers. Our optimized recipe yield is monodisperse DMAB-AuNBPs with ∼90% purity even at large scales (500 mL volume per batch). DMAB-AuNBPs show better cell viability (more than 90%) across all concentrations (5−500 ug/mL) when directly compared to CTAB-AuNBPs (less than 10%). Our findings show the potential of DMAB-AuNBPs for early stage cancer detection and theranostics applications.
“…Although AuNPs have previously been reported for the delivery of DOX [ 41 , 42 , 43 ], this is the first study reporting the use of dendronized AuNPs bearing both acid-labile DOX and a Fab ligand targeting prostate tumors.…”
Dendronized nanoparticles, also called nanoparticle-cored dendrimers, combine the advantages of nanoparticles and dendrimers. These very stable and polyvalent nanoparticles can be used for diverse applications. One such application is drug delivery, because the dendrons can enhance the density of the payload. In this report, we describe the design of multifunctional gold nanoparticles (AuNPs) coated with poly(propylene imine) (PPI) dendrons that contain both prostate cancer active targeting and chemotherapeutic drugs. The PPI dendron is a good candidate for the design of drug delivery vehicles because of its ability to induce a proton sponge effect that will enhance lysosomal escape and intracellular therapeutic delivery. The chemotherapeutic drug used is doxorubicin (DOX), and it was linked to the dendron through a hydrazone acid-sensitive bond. Subsequent acidification of the AuNP system to a pH of 4–5 resulted in the release of 140 DOX drugs per nanoparticles. In addition, the PPI dendron was conjugated via “click” chemistry to an EphA2-targeting antibody fragment that has been shown to target prostate cancer cells. In vitro cell viability assays revealed an IC50 of 0.9 nM for the targeted DOX-bearing AuNPs after 48 h incubation with PC3 cells. These results are very promising upon optimization of the system.
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