Laser Doppler Flow-Meter Assessment of Iontophoretically Applied Norepinephrine on Human Finger Skin Circulation

Lindblad, Lars Erik; Ekenvall, Lena; Ancker, Klas; Rohman, H.; Öberg, Peter

doi:10.1111/1523-1747.ep12456288

Cited by 38 publications

(17 citation statements)

References 4 publications

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“…An optical fiber that delivers the light to the tissue and 1 mm 3 of tissue contributes to the obtained signals. Blood flow sampling is restricted to the cutaneous vessels (Lindblad et al 1986). The frequency and power of the reflected photons are analyzed to yield relative estimates of blood volume and flow velocity.…”

Section: Cutaneous Vascular Responsiveness To Exercisementioning

confidence: 99%

Effects of exercise training and detraining on cutaneous microvascular function in man: the regulatory role of endothelium-dependent dilation in skin vasculature

Wang

2004

Eur J Appl Physiol

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This study investigated how exercise training and detraining affect the cutaneous microvascular function and the regulatory role of endothelium-dependent dilation in skin vasculature. Ten healthy sedentary subjects cycled on an ergometer at 50% of maximal oxygen uptake (VO(2max)) for 30 min daily, 5 days a week, for 8 weeks, and then detrained for 8 weeks. Plasma nitric oxide (NO) metabolites (nitrite plus nitrate) were measured by a microplate fluorometer. The cutaneous microvascular perfusion responses to six graded levels of iontophoretically applied 1% acetylcholine (ACh) and 1% sodium nitroprusside (SNP) in the forearm skin were determined by laser Doppler. After training, (1) resting heart rate and blood pressure were reduced, whereas VO(2max), skin blood flow and cutaneous vascular conductance to acute exercise were enhanced; (2) plasma NO metabolite levels and ACh-induced cutaneous perfusion were increased; (3) skin vascular responses to SNP did not change significantly. However, detraining reversed these effects on cutaneous microvascular function and plasma NO metabolite levels. The results suggest that endothelium-dependent dilation in skin vasculature is enhanced by moderate exercise training and reversed to the pretraining state with detraining.

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Section: Cutaneous Vascular Responsiveness To Exercisementioning

confidence: 99%

Effects of exercise training and detraining on cutaneous microvascular function in man: the regulatory role of endothelium-dependent dilation in skin vasculature

Wang

2004

Eur J Appl Physiol

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“…With the woman resting in a semisupine position in a room with an ambient temperature of 21.1°C, a probe holder for combined iontophoresis and blood flow measurement (Lindblad et al 1986) was fixed on the volar side of the forearm. The probe holder has a small chamber for deposition of the test substance which is then in direct proximity to the light fibres used to detect blood flow.…”

Section: Iontophoresis Of Acetylcholine and Sodium Nitroprussidementioning

confidence: 99%

Noninvasive test of microvascular endothelial function in normal and hypertensive pregnancies

Eneroth-Grimfors¹,

Lindblad²,

Westgren³

et al. 1993

BJOG

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Objective To examine microvascular endothelial cell function in vivo in pre‐eclampsia. Design Iontophoresis of acetylcholine (Ach), which gives rise to endothelial cell dependent vasodilatation, and of sodium nitroprusside (SNP), which elicits vasodilatation independently of functioning vascular endothelium. Setting Södersjukhuset, Stockholm, Sweden. Subjects Ten pre‐eclamptic patients, ten healthy pregnant women and ten healthy nonpregnant women were examined. Main outcome measures The degree of vasodilatation following iontophoretic administration of Ach compared with SNP was recorded with a laser Doppler technique, the data being analysed on a personal computer. Results Both Ach and SNP administration resulted in marked vasodilatation; the magnitude of the vasodilatation was similar in the three groups of women. Conclusion Following iontophoretic administration of endothelial cell dependent or independent vasodilatators, laser Doppler measurement of blood flow demonstrated no microvascular endothelial cell dysfunction in pre‐eclamptic women.

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“…It is clear that under such circumstances area measurement is not ap propriate for evaluating drug efficiency. La ser Doppler flowmetry has been developed for probing the skin microvasculature in a noninvasive way and has been shown to give almost equivalent results when compared with more invasive methods such as xenon-133 washout kinetics [6], Several groups ap proached quantitative evaluation of skin mi crovasculature with this method in evaluat ing experimentally topically administered inflammatory agents [7] or vasoactive sub stances [8][9][10][11], In dermatology clinics it is also used for quantitative assessment of skin irritation [12,13] or delayed-type hypersen sitivity contact dermatitis [14]. A review of these and related subjects is given by Steven son et al [15].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of the Cutaneous Response to Histamine by H<sub>1</sub>-Blocking Agents

Neste¹,

Rihoux²

1988

Skin Pharmacol Physiol

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A controlled, randomized, double-blind, cross-over study was performed in 10 healthy volunteers in order to compare changes of cutaneous blood flow values (CBFV, laser Doppler flowmetry, Periflux, Perimed, S) in histamine-challenged skin before and after administration of a capsule containing either 10 mg cetirizine or 60 mg terfenadine. The overall pattern of the basal cutaneous wheal and flare response to the agonists (histamine: aqueous solution: Hsol, or Phazet®: Hprick) and control saline solution, showed the expected range of clinical effects, i.e. Hsol > Hprick > saline. Similarly, the increase of CBFV on the flare area was higher around the histamine injections as compared with saline. Surprisingly, CBFV was decreased at the site of Hsol and Hprick injections as compared with the levels recorded at the control injections. The following changes were noted after intake of H₁-blocking agents. (1) There was a reduction of the areas of wheal and flare at all injection sites after administration of the H₁ blockers, this reduction being consistently greater under cetirizine than terfenadine. (2) CBFV, measured 1 cm from the site of Hsol injection (i.e., within the area of normal flare response), was decreased only with cetirizine. In the smaller flare area induced by Hprick, CBFV was equally suppressed by both drugs. (3) At the site of agonist injection, along with a reduction of the size of the wheal, we observed significant increases in CBFV after drug intake. Under these experimental conditions, this quantitative pharmacologic in vivo assay of agonist-antagonist interactions suggests that after initial administration of normally prescribed doses, cetirizine may possess more anti-H₁ activity than terfenadine.

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Laser Doppler Flow-Meter Assessment of Iontophoretically Applied Norepinephrine on Human Finger Skin Circulation

Cited by 38 publications

References 4 publications

Effects of exercise training and detraining on cutaneous microvascular function in man: the regulatory role of endothelium-dependent dilation in skin vasculature

Effects of exercise training and detraining on cutaneous microvascular function in man: the regulatory role of endothelium-dependent dilation in skin vasculature

Noninvasive test of microvascular endothelial function in normal and hypertensive pregnancies

Inhibition of the Cutaneous Response to Histamine by H<sub>1</sub>-Blocking Agents

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