Laser Capture Microdissection of Cells from Plant Tissues

Kerk, Nancy M.; Ceserani, Teresa; Tausta, S Lori; Sussex, Ian M.; Nelson, Timothy

doi:10.1104/pp.102.018127

Cited by 300 publications

(300 citation statements)

References 20 publications

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“…nucleic acids, proteins and/or metabolites) from the harvested cells. The preparation of plant samples has been clearly illustrated in several original papers (Asano et al 2002;Kerk et al 2003;Klink et al 2005;Inada and Wildermurth 2005;Tang et al 2006;Cai and Lashbrook 2006) and in several reviews (Day et al 2005(Day et al , 2006Nelson et al 2006;Balestrini and Bonfante 2008;). Two methods have been utilized to prepare sample sections for LM: cryosectioning and paraffin sectioning.…”

Section: How Laser Microdissection Operatesmentioning

confidence: 99%

Application of Laser Microdissection to plant pathogenic and symbiotic interactions

Balestrini¹,

Gómez-Ariza²,

Klink

et al. 2009

Journal of Plant Interactions

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Laser Microdissection (LM) is a technology that allows the rapid procurement of selected cell populations from a section of heterogeneous tissues in a manner conducive to the extraction of DNA, RNA, proteins and even metabolites. In the past few years, it has also been applied to plant biology in order to study gene expression in plant-nematode and plant-microbe interactions. LM represents a powerful tool since cells associated with a particular infection stage can be visualized under the microscope and harvested. Therefore, verification of the response of the plant during the progression of the colonization can be performed in different cell types. Applications of LM to study the interaction between the plant and both pathogenic and symbiotic organisms (i.e. nematode and fungi, respectively) are explored in this review.

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Section: How Laser Microdissection Operatesmentioning

confidence: 99%

Application of Laser Microdissection to plant pathogenic and symbiotic interactions

Balestrini¹,

Gómez-Ariza²,

Klink

et al. 2009

Journal of Plant Interactions

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“…5). LCM technology has been used successfully in rice (Oryza sativa), maize (Zea mays), tobacco, soybean, and Arabidopsis in which a variety of plant tissues and cell types have been isolated and studied, including those in an early Arabidopsis embryo (Asano et al, 2002;Kerk et al, 2003;Nakazono et al, 2003;Casson et al, 2005;Klink et al, 2005;Sanders et al, 2005;Spencer et al, 2007).…”

Section: Using Soybean To Identify Genes Required To Make a Seedmentioning

confidence: 99%

Using Genomics to Study Legume Seed Development

Wagmaister²,

Kawashima³

et al. 2007

Plant Physiology

147

157

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“…However, both types of cells are difficult to isolate by conventional microdissection methods in the numbers required for gene expression profiling. Several physical or biochemical methods for the isolation of specific cell or tissue types for RNA analysis have been reported recently (Karrer et al, 1995;Brandt et al, 1999;Birnbaum et al, 2003;Kerk et al, 2003). However, these methods typically produce only small numbers of cells and require amplification of the extracted RNA prior to microarray hybridization (Klur et al, 2004), a process that results in significant loss of information with only approximately 37% of the RNA being amplified.…”

mentioning

confidence: 99%

Genome-Wide Identification of Genes Expressed in Arabidopsis Pistils Specifically along the Path of Pollen Tube Growth

et al. 2005

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Plant reproductive development is dependent on successful pollen-pistil interactions. In crucifers, the pollen tube must breach the stigma surface and burrow through the extracellular matrix of the stigma epidermal cells and transmitting tract cells before reaching its ovule targets. The high degree of specificity in pollen-pistil interactions and the precision of directional pollen tube growth suggest that signals are continually being exchanged between pollen/pollen tubes and cells of the pistil that line their path. However, with few exceptions, little is known about the genes that control these interactions. The specialized functions of stigma epidermal cells and transmitting tract cells are likely to depend on the activity of genes expressed specifically in these cells. In order to identify these genes, we used the Arabidopsis (Arabidopsis thaliana) ATH1 microarray to compare the whole-genome transcriptional profiles of stigmas and ovaries isolated from wild-type Arabidopsis and from transgenic plants in which cells of the stigma epidermis and transmitting tract were specifically ablated by expression of a cellular toxin. Among the 23,000 genes represented on the array, we identified 115 and 34 genes predicted to be expressed specifically in the stigma epidermis and transmitting tract, respectively. Both gene sets were significantly enriched in predicted secreted proteins, including potential signaling components and proteins that might contribute to reinforcing, modifying, or remodeling the structure of the extracellular matrix during pollination. The possible role of these genes in compatible and incompatible pollen-pistil interactions is discussed.

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Laser Capture Microdissection of Cells from Plant Tissues

Cited by 300 publications

References 20 publications

Application of Laser Microdissection to plant pathogenic and symbiotic interactions

Application of Laser Microdissection to plant pathogenic and symbiotic interactions

Using Genomics to Study Legume Seed Development

Genome-Wide Identification of Genes Expressed in Arabidopsis Pistils Specifically along the Path of Pollen Tube Growth

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