Application of Laser Microdissection to plant pathogenic and symbiotic interactions

Abstract

Laser Microdissection (LM) is a technology that allows the rapid procurement of selected cell populations from a section of heterogeneous tissues in a manner conducive to the extraction of DNA, RNA, proteins and even metabolites. In the past few years, it has also been applied to plant biology in order to study gene expression in plant-nematode and plant-microbe interactions. LM represents a powerful tool since cells associated with a particular infection stage can be visualized under the microscope and harvested. Therefore, verification of the response of the plant during the progression of the colonization can be performed in different cell types. Applications of LM to study the interaction between the plant and both pathogenic and symbiotic organisms (i.e. nematode and fungi, respectively) are explored in this review.

Differential spatio-temporal expression of carotenoid cleavage dioxygenases regulates apocarotenoid fluxes during AM symbiosis

“…Laser microdissection (LMD) offers an effective way to check gene expression in individual cells [60,61]. This is particularly important considering the asynchronous character of the AM symbiosis.…”

Symbiotic Proteomics — State of the Art in Plant–Mycorrhizal Fungi Interactions

“…Another aspect that has hindered the use of proteomics in the study of mycorrhizal interactions is the fact that the mycorrhizal symbiosis involves a small percentage of plant root cells, that may contain fungal structures at different developmental stages and with different putative roles [29]. For example, arbuscules are limited to the root cortical cells in the AM symbiosis, a tissue where not all plant cells are colonized.…”

“…In order to identify proteins from a very small subset of target cells, Gaude et al (2012) combined laser capture microdissection (LCM) and LC-MS/MS [74]. Laser microdissection permits the rapid isolation, from sections of a heterogeneous tissue, of a selected cell population in a manner compatible with the extraction of DNA, RNA or proteins [29]. Using LCM, arbuscule-containing cortical cells and cortical cells from non-mycorrhizal M. truncatula roots were isolated.…”

Monochamus Saltuarius Endangers Pinus tabuliformis Carr. and Carries Bursaphelenchus xylophilus (Steiner and Buhrer) in China

“…Monochamus beetles can carry a large number of four-stage dispersal juveniles in their surface respiratory trachea and reproductive system after eclosion from pines killed by pine wilt disease. These Monochamus migrate to healthy pine trees, thereby spreading the pine wilt disease when feeding on the tender branches [5][6][7].…”