Symbiotic Proteomics — State of the Art in Plant–Mycorrhizal Fungi Interactions

Chiapello, Marco; Perotto, Silvia; Balestrini, Raffaella

doi:10.5772/61331

Cited by 4 publications

(4 citation statements)

References 82 publications

(100 reference statements)

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“…Mutualistic fungi secrete also various effector proteins that repress plant defence responses. After entering the plant cell, some effector proteins are transported to the plant nucleus, where they can interact with transcription factors and repress plant defence [15]. This is well studied for the small secreted protein 7 (SP7) from Rhizophagus irregularis and MISSP7 (mycorrhiza-induced small secreted protein-7) from Laccaria bicolor [16–18].…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis thaliana responds to colonisation of Piriformospora indica by secretion of symbiosis-specific proteins

et al. 2018

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Plants interact with a wide variety of fungi in a mutualistic, parasitic or neutral way. The associations formed depend on the exchange of nutrients and signalling molecules between the partners. This includes a diverse set of protein classes involved in defence, nutrient uptake or establishing a symbiotic relationship. Here, we have analysed the secretomes of the mutualistic, root-endophytic fungus Piriformospora indica and Arabidopsis thaliana when cultivated alone or in a co-culture. More than one hundred proteins were identified as differentially secreted, including proteins associated with growth, development, abiotic and biotic stress response and mucilage. While some of the proteins have been associated before to be involved in plant-microbial interaction, other proteins are newly described in this context. One plant protein found in the co-culture is PLAT1 (Polycystin, Lipoxygenase, Alpha-toxin and Triacylglycerol lipase). PLAT1 has not been associated with plant-fungal-interaction and is known to play a role in abiotic stress responses. In colonised roots PLAT1 shows an altered gene expression in a stage specific manner and plat1 knock-out plants are colonised stronger. It co-localises with Brassicaceae-specific endoplasmic reticulum bodies (ER-bodies) which are involved in the formation of the defence compound scopolin. We observed degraded ER-bodies in infected Arabidopsis roots and a change in the scopolin level in response to the presence of the fungus.

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Section: Introductionmentioning

confidence: 99%

Arabidopsis thaliana responds to colonisation of Piriformospora indica by secretion of symbiosis-specific proteins

et al. 2018

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“…Although recent genetics and molecular studies have allowed the identification of many players regulating the complex plant‐ fungal interactions, little information is so far available on the proteomic factors involved in AM symbiosis [29]. The symbiotic association between plants and AM fungi significantly interferes with the plant physiology, especially in roots, leading to deep protein expression changes [19]. We used a meta‐analysis platform to identify key proteins and biological processes regulated by the symbiosis in AM plants.…”

Section: Resultsmentioning

confidence: 99%

“…Thanks to many transcriptomics analyses, an extensive plant gene expression modulation has been described in AM plants with up to thousands of differentially expressed genes, while by contrast, in ECM roots, gene expression was found to be more extensively regulated in the fungus than in the plant (up to 20% of the analyzed transcripts in the fungus with respect to 2-5% in the plant) [1]. Despite these evidences and the many recent reviews [5,[17][18][19], several molecular mechanisms underlying the mycorrhiza-related plant performance promotion are still poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Proteomics as a tool to decipher plant responses in arbuscular mycorrhizal interactions: a meta‐analysis

et al. 2023

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The beneficial symbiosis between plants and arbuscular mycorrhizal (AM) fungi leads to a deep reprogramming of plant metabolism, involving the regulation of several molecular mechanisms, many of which are poorly characterized. In this regard, proteomics is a powerful tool to explore changes related to plant–microbe interactions. This study provides a comprehensive proteomic meta‐analysis conducted on AM‐modulated proteins at local (roots) and systemic (shoots/leaves) level. The analysis was implemented by an in‐depth study of root membrane‐associated proteins and by a comparison with a transcriptome meta‐analysis. A total of 4262 differentially abundant proteins were retrieved and, to identify the most relevant AM‐regulated processes, a range of bioinformatic studies were conducted, including functional enrichment and protein‐protein interaction network analysis. In addition to several protein transporters which are present in higher amounts in AM plants, and which are expected due to the well‐known enhancement of AM‐induced mineral uptake, our analysis revealed some novel traits. We detected a massive systemic reprogramming of translation with a central role played by the ribosomal translational apparatus. On one hand, these new protein‐synthesis efforts well support the root cellular re‐organization required by the fungal penetration, and on the other they have a systemic impact on primary metabolism.

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“…Recently a new concept on use of symbiotic proteomics tools for better understanding of the molecular bases of cell communication and the regulation of developmental and metabolic pathways in mycorrhizal associations were established for general increase in stress tolerance and health. Chapelo et al [21] studied that proteomics gives a better understanding to identify proteins expressed and regulated during the development and functioning of mycorrhizal symbioses, and thus contribute in information for events occurring at the cellular level…”

Section: Practical Application Of Omics In Phytoremediation Technology Has Been Represented In the Tablementioning

confidence: 99%

Proteomics and Bioinformatics as Novel Tools in Phytoremediation Technology- An Overview

Mohanty

2021

j. of bot. res.

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Biotechnology plays an important role in mitigation of various pollution in a cost effective manner by using the complex chemistry of living organisms, various cell manipulations and their approaches for environmental cleanup along with environmental sustainability. One such technology is phytoremediation technology or green technology which has emerged and evolved as a novel tool for remediation of toxic contaminants from environment. Plants with its diverse range show a remarkable range of their phytoremediation potentiality for establishing a sustainable environment. There is a huge exploitation of natural resources through expanded industrialization, urbanization, modern agricultural development, energy generation to fulfill the never-ending human desires and need. This disturbs the balance in nature where we reside and leads to progressive deterioration of the environment. There are several biotechnological advances which are employed for combating both the biotic and abiotic stress problems caused due to toxic contaminants in the environment. Various biotechnological interventions such as bioinformatics, proteomics, genomics, metallomics and metabolomics play a crucial role and open new avenue in this context. This omics approach is now integrated with bioinformatics to serve as a novel tool in phytoremediation technology. This smart technology provides insights into the complex behavior of enzymes, proteins and metabolites action and their biochemical pathways for degradation of wastes. This leads towards deriving a sustainable solution for environmental pollution.

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Symbiotic Proteomics — State of the Art in Plant–Mycorrhizal Fungi Interactions

Cited by 4 publications

References 82 publications

Arabidopsis thaliana responds to colonisation of Piriformospora indica by secretion of symbiosis-specific proteins

Arabidopsis thaliana responds to colonisation of Piriformospora indica by secretion of symbiosis-specific proteins

Proteomics as a tool to decipher plant responses in arbuscular mycorrhizal interactions: a meta‐analysis

Proteomics and Bioinformatics as Novel Tools in Phytoremediation Technology- An Overview

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