Laser Ablation Synthesis in Solution and Nebulization of Silver-109 Nanoparticles for Mass Spectrometry and Mass Spectrometry Imaging

Abstract

Preparation of monoisotopic silver-109 nanoparticles ( 109 AgNPs) by pulsed fiber laser (PFL) ablation synthesis in solution (LASiS) with the use of a 2D galvoscanner (2D GS) is described. The procedure of covering of custom-made stainless-steel MALDI targets containing studied objects via nebulization is also presented. Examples of application of the new method (PFL-2D GS LASiS and nebulization) in mass spectrometry (MS) analyses and MS imaging (MSI) are shown. These include tests with a nonionic nucleoside and saccharide, ionic amino acids, and also a low-molecular-weight polymer. Fingerprint MS imaging is shown as an example of a fast and simple MSI procedure.

Antibacterial Activity of Bismuth Oxide Nanoparticles Compared to Amikacin against Acinetobacter baumannii and Staphylococcus aureus

“…Surface roughness [30] and topography have all been effective in microbial biofilm research [31]. A few studies have shown that Bi 2 O 3 NPs have strong antibiofilm actions against various pathogens in vitro and have indicated various antibacterial mechanisms for Bi 2 O 3 NPs, including impairing cell division, disturbing the cell wall cytoplasmic membrane, and decomposing DNA [32][33][34]. By employing AFM image analysis [29], the biofilm formation and surface roughness of P. aeruginosa were investigated by AFM.…”

Infrared pulsed fiber laser‐produced silver‐109‐nanoparticles for laser desorption/ionization mass spectrometry of amino acids

“…It is also cost-effective, as no other chemicals are required and the silver-109 metal foil can be reused. 15…”

Infrared pulsed fiber laser‐produced silver‐109 nanoparticles for laser desorption/ionization mass spectrometry of 3‐hydroxycarboxylic acids

“…6 Laser ablation methods have an advantage over chemical synthesis because they allow for very quick synthesis of NPs (several seconds vs. hours in chemical synthesis), and the synthesized NPs are stabilizer-and reducingagent-free. 7 The literature provides many examples of the use of NPs in LMW analysis.…”

“…Moreover, monoisotopic silver NPs have some additional properties like the simplest possible peak pattern, a much higher intensity of analyte peaks, and simplified calculations of isotopic distribution 6 . Laser ablation methods have an advantage over chemical synthesis because they allow for very quick synthesis of NPs (several seconds vs. hours in chemical synthesis), and the synthesized NPs are stabilizer‐ and reducing‐agent‐free 7 . The literature provides many examples of the use of NPs in LMW analysis.…”

“…RESULTS AND DISCUSSIONThe method of synthesis of laser-generated nanomaterial (LGN) with PFL 2D GS laser, properties of silver-109 NPs, and their application are available in our recent publication 7. This work presents the use of laser-generated 109 AgNPs for qualitative and quantitative analysis of 3-hydroxycarboxylic acids.…”