Infrared pulsed fiber laser‐produced silver‐109‐nanoparticles for laser desorption/ionization mass spectrometry of amino acids

Abstract

Application of monoisotopic cationic 109Ag nanoparticles (109AgNPs) obtained by pulsed fiber laser (PFL) 2D galvo‐scanner (GS) laser generated nanomaterial (LGN) for both high resolution laser desorption/ionization mass spectrometry and mass spectrometry imaging of amino acids is presented. Four amino acids, alanine, isoleucine, lysine, and phenylalanine were used as test compounds for quantification with matrix‐assisted laser desorption/ionization mas (MALDI)‐type mass spectrometer. Comparison of commonly made manual measurements with semiautomatic mass spectrometry imaging (MSI) was performed providing very interesting findings. Amino acids were directly tested in 1 000 000‐fold concentration change conditions ranging from 1 mg/ml to 1 ng/ml, which equates to 500 ng to 500 fg of amino acid per measurement spot. Methods were also tested on samples of human blood plasma for quantification of endogenous amino acids.

Fiber Laser-Generated Silver-109 Nanoparticles for Laser Desorption/Ionization Mass Spectrometry of Illicit Drugs

“…Silver-109 nanoparticle-enhanced LDI applicability for samples of various compositions has been presented by our team in the past. 19,21,25 Obtained mass spectra are presented in Figures 1−3. The list of identified ions with the average signal intensity and signal-to-noise ratio of the analyzed compounds at a concentration of 100 μg/mL is presented in Table 1.…”

“…In this research, the applicability of 109 AgNPs-assisted laser desorption/ionization MS for identification and quantification of fentanyl and other drugs, such as THC and its two metabolites, as well as opioids, including tramadol, 6-monoacetylmorphine (6-MAM), codeine, methadone, and heroin, was presented. Silver-109 nanoparticle-enhanced LDI applicability for samples of various compositions has been presented by our team in the past. ,, Obtained mass spectra are presented in Figures –. The list of identified ions with the average signal intensity and signal-to-noise ratio of the analyzed compounds at a concentration of 100 μg/mL is presented in Table .…”

“…In this study, the applied analytical method allowed for rapid screening for fentanyl and other illicit drugs. The sample preparation for 109 AgNP-assisted LDI MS is a matter of minutes, with the spectral data acquisition itself taking even less time. − Recently, our research has successfully applied this technique for both qualitative and quantitative analysis of low molecular weight compounds, including amino acids, − carboxylic acids, − and hormones . Furthermore, this method has shown exceptional utility in analyzing microorganisms − and various human biological samples such as serum, urine, , and tissue.…”

Infrared pulsed fiber laser‐produced gold and silver‐109 nanoparticles for laser desorption/ionization mass spectrometry of steroid hormones

“…In general, LGN produces suspensions of a relatively high chemical purity so leading to simple mass spectra with low chemical background 25 . Recent publications reported the results of the quantitative analysis of amino acids and carboxylic acids with the use of monoisotopic AgNPs obtained with the LGN method 26,27 …”

Laser Generated Gold Nanoparticles for Mass Spectrometry of Low Molecular Weight Compounds

“…Another way NPs can also be obtained by breaking up a larger structure, for example with a laser, which is classified as physical method [13,14]. The method of generating nano-particles by means of a laser (LGN) has many advantages, owing to which the obtained NPs are successfully used in mass spectrometry [20][21][22]. LGN uses pulsed laser irradiation to ablate a target from a solid material immersed in a liquid, ejecting NPs from the plasma cloud into the surrounding solution.…”