Laser Ablation of<i>Drosophila</i>Embryonic Motoneurons Causes Ectopic Innervation of Target Muscle Fibers

Chang, Te Ning; Keshishian, Haig

doi:10.1523/jneurosci.16-18-05715.1996

Cited by 34 publications

(33 citation statements)

References 70 publications

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“…Staining of 3 rd -instar larval synapses with anti-CSP also revealed that the placement of motoneuron terminals on abdominal wall muscles is normal in sax and Mad mutants (data not shown). When muscles are innervated ectopically by incorrect motoneurons the placement of the synapses is abnormal (Chang et al, 1996). These studies of embryos and 3 rd -instar larvae suggest that correct innervation of muscles 6 and 7 by the RP3 motoneuron is achieved in sax and Mad mutants.…”

Section: Mad and Sax Mutants Have Impaired Nmj Morphology And Functionmentioning

confidence: 76%

Drosophila neuromuscular synapse assembly and function require the TGF‐β type I receptor saxophone and the transcription factor Mad

et al. 2003

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Transforming growth factor-betas (TGF-beta) comprise a superfamily of secreted proteins with diverse functions in patterning and cell division control. TGF-beta signaling has been implicated in synapse assembly and plasticity in both vertebrate and invertebrate systems. Recently, wishful thinking, a Drosophila gene that encodes a protein related to BMP type II receptors, has been shown to be required for the normal function and development of the neuromuscular junction (NMJ). These findings suggest that a TGF-beta-related ligand activates a signaling cascade involving type I and II receptors and the Smad family of transcription factors to orchestrate the assembly of the NMJ. Here we demonstrate that the TGF-beta type I receptor Saxophone and the downstream transcription factor Mothers against dpp (Mad) are essential for the normal structural and functional development of the Drosophila NMJ, a synapse that displays activity-dependent plasticity.

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Section: Mad and Sax Mutants Have Impaired Nmj Morphology And Functionmentioning

confidence: 76%

Drosophila neuromuscular synapse assembly and function require the TGF‐β type I receptor saxophone and the transcription factor Mad

et al. 2003

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“…For example, the stereotypic neuromuscular connections in Drosophila larvae are able to reorganize after the ablation of a specific motoneuron during development. The corresponding target muscle can receive synaptic input from neighboring motoneurons as a result of axonal sprouting of neighboring motor neurons (Chang and Keshishian, 1996). Likewise in the Drosophila visual system, photoreceptor axons can sprout into neighboring empty space created by a cell fate transformation (Ashley and Katz, 1994).…”

Section: Stability Of the Olfactory Circuitmentioning

confidence: 99%

Wiring Stability of the AdultDrosophilaOlfactory Circuit after Lesion

Berdnik¹,

Chihara²,

Couto³

et al. 2006

J. Neurosci.

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Neuronal wiring plasticity in response to experience or injury has been reported in many parts of the adult nervous system. For instance, visual or somatosensory cortical maps can reorganize significantly in response to peripheral lesions, yet a certain degree of stability is essential for neuronal circuits to perform their dedicated functions. Previous studies on lesion-induced neuronal reorganization have primarily focused on systems that use continuous neural maps. Here, we assess wiring plasticity in a discrete neural map represented by the adult Drosophila olfactory circuit. Using conditional expression of toxins, we genetically ablated specific classes of neurons and examined the consequences on their synaptic partners or neighboring classes in the adult antennal lobe. We find no alteration of connection specificity between olfactory receptor neurons (ORNs) and their postsynaptic targets, the projection neurons (PNs). Ablating an ORN class maintains PN dendrites within their glomerular borders, and ORN axons normally innervating an adjacent target do not expand. Likewise, ablating PN classes does not alter their partner ORN axon connectivity. Interestingly, an increase in the contralateral ORN axon terminal density occurs in response to the removal of competing ipsilateral ORNs. Therefore, plasticity in this circuit can occur but is confined within a glomerulus, thereby retaining the wiring specificity of ORNs and PNs. We conclude that, although adult olfactory neurons can undergo plastic changes in response to the loss of competition, the olfactory circuit overall is extremely stable in preserving segregated information channels in this discrete map.

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“…Laser beams have the advantage of high spatial and temporal precision (Shah and Jay, 1993), and are frequently used for selective cell elimination. In recent years, laser beams have been used to kill specific cells in Caenorhabditis elegans, Drosophila, amphibians, grasshoppers, and zebra fish to investigate cell function, cell regeneration, and cell-cell interactions (Bargmann and Avery, 1995;Chang and Keshishian, 1996;Balak et al, 1990;Kuwada, 1993;Shah and Jay, 1993). Laser beams have even been used to damage specific dyelabeled cellular components without destroying the cell (Jay and Keshishian, 1990).…”

Section: Introductionmentioning

confidence: 99%

Laser ablation reveals regulation of ciliary activity by serotonergic neurons in molluscan embryos

Kuang

Goldberg

2001

J. Neurobiol.

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Early in embryonic development, the pond snail Helisoma trivolvis exhibits a rotational behavior that is generated by beating of cilia in the dorsolateral and pedal bands. Although previous anatomical and pharmacological studies provided indirect evidence that a pair of serotonergic neurons, Embryonic Neurons C1 (ENC1s), is involved in regulating embryonic rotation, direct evidence linking ENC1 to ciliary function is still lacking. In the present study, we used laser microbeams to perturb ENC1 in vivo while monitoring ciliary activity in identified ciliary bands. A laser treatment protocol to specifically ablate ENC1 without damaging the surrounding cells was established. Unilateral laser treatment of ENC1 caused transient increases in the activity of the pedal and ipsidorsolateral cilia, lasting 30-50 min. In contrast, activity of cilia that were not anatomically associated with ENC1 was unaffected by laser treatment. Mianserin, an effective serotonin antagonist in Helisoma ciliated cells, decreased the overall CBF of pedal and dorsolateral cilia by reducing the occurrence of spontaneous CBF surges in these cilia. Finally, the cilioexcitatory action of ENC1 laser treatment was mimicked by serotonin and reduced in the presence of mianserin. These results suggest that laser treatment provokes a release of serotonin from ENC1, resulting in a prolonged elevation of activity in the target ciliary cells. We conclude that, in addition to their previously established role in regulating neurodevelopment, ENC1s also function as serotonergic motor neurons to regulate ciliary activity, and therefore the rotational behavior of early embryos.

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Laser Ablation ofDrosophilaEmbryonic Motoneurons Causes Ectopic Innervation of Target Muscle Fibers

Cited by 34 publications

References 70 publications

Drosophila neuromuscular synapse assembly and function require the TGF‐β type I receptor saxophone and the transcription factor Mad

Drosophila neuromuscular synapse assembly and function require the TGF‐β type I receptor saxophone and the transcription factor Mad

Wiring Stability of the AdultDrosophilaOlfactory Circuit after Lesion

Laser ablation reveals regulation of ciliary activity by serotonergic neurons in molluscan embryos

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