Las proteínas del plasma seminal incrementan la viabilidad espermática post-descongelación del semen de toros Sanmartinero

A, Fabián Rueda; P, Tatiana Garcés; L, Rocío Herrera; R, Luis Arbeláez; J, Miguel Peña; P, Henry Velásquez; Hernández, Aureliano; C, Jaime Cardozo

doi:10.21897/rmvz.195

Cited by 6 publications

(6 citation statements)

References 21 publications

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“…Promising results have been reported by Pan et al (23) which used an extender with SP concentration of 25% leading to effective preservation of the canine sperm motility and plasma membrane integrity in samples chilled at 4 °C. The inclusion of SP proteins with a molecular weight of 14 kDa and 16 kDa and isoelectric points between 5.0-5.5 in the preservation medium increased bull sperm viability (26). The use of seminal plasma proteins in the range of 12-20 kDa leads to improvement of biotechnological preservation of spermatozoa (27).…”

Section: Discussionmentioning

confidence: 99%

Effect of Seminal Plasma Protein Fractions on Cooled Dog Semen Kinetics

Tsvetkov,

Daskalova

2023

Macedonian Veterinary Review

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Semen cooling and cryopreservation have revolutionized the field of reproductive biotechnologies. However, challenges persist in maintaining sperm quality and viability during these processes. The unsatisfactory results and the main problems are associated with low quality, viability, morphological, structural, and DNA integrity, changes in plasma membrane, ability to interact with female tract and decrease in sperm fertilization potential. The objective of this study was to determine the effect of seminal plasma proteins with different molecular weights on the kinetic parameters of dog spermatozoa during cold storage at 4 °C. The proteins were isolated from the dogs’ seminal plasma ranging between 6-200 kDa, separated in four fractions. The ejaculates (n=15) were collected from 6 heathy dogs which were pooled. Spermatozoa were extended with Tris medium alone (control) or with addition of one of the isolated protein fractions, and were then incubated at 4 °C for 2 hours. Sperm incubated with seminal plasma proteins ranging between 10-15 kDa had significantly higher total motility (37.01±2.98%), sperm progressiveness (15.97±1.91%), curvilinear line velocity (37.46±3.75 μm/s), linearity (26.18±1.00%), and straightness (45.94±2.03%) compared to the other groups incubated with higher molecular weight proteins and the control group. The findings of this study indicated that the use of certain proteins in seminal plasma can be beneficial for reducing the detrimental effect of cooling at 4 °C and preserving the viability of dog spermatozoa. It seemed that the presence of 10-15 kDa proteins from canine seminal plasma rendered the spermatozoa less amenable to the negative influence during cooling.

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Section: Discussionmentioning

confidence: 99%

Effect of Seminal Plasma Protein Fractions on Cooled Dog Semen Kinetics

Tsvetkov,

Daskalova

2023

Macedonian Veterinary Review

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“…At the same time, the excessive concentration of proteins competing for these sites may result in a lower amount of SP proteins bound to their target sites, resulting in lack of effect. Several authors have reported significant improvements in PM integrity of frozen‐thawed spermatozoa, washed or the unwashed, with SP or some SP proteins (Barrios, ; Leahy et al, ; Pérez‐Pé et al, ; Rueda A et al, ). However, unwashed sperm incubated with ‘raw or complete’ SP did not achieve PM integrity rates higher than 28% (Leahy et al, ; Rovegno et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…The ability of SP proteins to reverse the cryodamage has been documented on live gametes, usually when SP was added after swim‐up or density gradient selection techniques (Bernardini et al, ; Maxwell et al, ; Rovegno et al, ; Rueda et al, ). This suggests that SP proteins show beneficial effects when they are in contact with cell suspension depleted from their own SP and extender components.…”

Section: Introductionmentioning

confidence: 99%

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Extenders modify the seminal plasma ability to minimize freeze‐thaw damage on ram sperm

Ramírez-Vasquez

Cesari

Greco

et al. 2019

Reprod Domestic Animals

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Seminal plasma (SP) proteins interact with sperm plasma membrane (PM) modulating its functionality. It has been shown that SP proteins can reverse the damage caused by freeze‐thaw; however in these studies, SP has been added to washed sperm (i.e., cells depleted from homologous SP and extender). The aim of the current study was to assess whether the egg yolk‐based extender (EY) modifies SP ability to ameliorate sperm parameters in frozen‐thawed ram spermatozoa. Ejaculates were diluted in EY or soybean lecithin‐based extender (SL) and evaluated before and after freezing to measure the cell damage according to the extender. Even when all classical parameters decreased after freezing, as expected (p < .05), there was no effect of the extender. SP treatment was applied after freeze‐thaw. Sperm were incubated with SP (20% v/v) in the presence of either EY or SL, and sperm parameters were assessed after thawing compared with the same treatments after Percoll sperm selection (washed). Treatments with 20% SP improved sperm total and progressive motility compared with controls regardless of washing and extender (p < .05); however, washed sperm showed higher percentage of total sperm motility compared with those unwashed (p < .05). Moreover, treatment with 20% SP showed significantly higher percentages of PM integrity, sperm with intact acrosomes, integrity of chromatin and non‐capacitated sperm in samples diluted with EY when washed before treatment compared with the other conditions (p < .05). It was concluded that the presence of the extenders and particularly egg yolk alters the SP capacity to reduce the cryodamage.

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“…This indicates that sperm quality can be improved or at least sustained by these specific proteins during cryopreservation [ 99 , 100 ]. Seminal plasma proteins having a low molecular weight (14–16 kDa) help maintain bovine sperm viability in a frozen medium [ 101 ]. Adding 1–1.5 mg of protein having M.W (14–16 kDa) elevated sperm viability by up to 20%.…”

Section: Addition Of Seminal Plasma Components and Other Supplementsmentioning

confidence: 99%

Effect of Sperm Cryopreservation in Farm Animals Using Nanotechnology

Akhtar

et al. 2022

Animals

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Sperm cryopreservation is one of the sublime biotechnologies for assisted reproduction. In recent decades, there has been an increasing trend in the use of preserved semen. Post-thaw semen quality and values vary among animals of the same species. Similarly, there are species-specific variations in sperm morphology, i.e., sperm head, kinetic properties, plasma membrane integrity, and freezability. Similarly, the viability of sperm varies in the female reproductive tract, i.e., from a few hours (in cattle) to several days (in chicken). Various steps of sperm cryopreservation, i.e., male health examination, semen collection, dilution, semen centrifugation, pre- and post-thaw semen quality evaluation, lack standardized methodology, that result in differences in opinions. Assisted reproductive technologies (ART), including sperm preservation, are not applied to the same extent in commercial poultry species as in mammalian species for management and economic reasons. Sperm preservation requires a reduction in physiological metabolism by extending the viable duration of the gametes. Physiologically and morphologically, spermatozoa are unique in structure and function to deliver paternal DNA and activate oocytes after fertilization. Variations in semen and sperm composition account for better handling of semen, which can aid in improved fertility. This review aims to provide an update on sperm cryopreservation in farm animals.

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Las proteínas del plasma seminal incrementan la viabilidad espermática post-descongelación del semen de toros Sanmartinero

Cited by 6 publications

References 21 publications

Effect of Seminal Plasma Protein Fractions on Cooled Dog Semen Kinetics

Effect of Seminal Plasma Protein Fractions on Cooled Dog Semen Kinetics

Extenders modify the seminal plasma ability to minimize freeze‐thaw damage on ram sperm

Effect of Sperm Cryopreservation in Farm Animals Using Nanotechnology

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