Extenders modify the seminal plasma ability to minimize freeze‐thaw damage on ram sperm

Ramírez-Vasquez, Rafael; Cesari, Andreína; Greco, Micaela; Cano, Adriana Virginia; Hozbor, F.

doi:10.1111/rda.13571

Cited by 13 publications

(7 citation statements)

References 49 publications

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“…In our study, we used concentrations (i.e., 0, 125, 250, and 500 µg/mL) based on a previous study that used a bovine model [ 40 ]. Results from different studies indicate that adding SP to proteins could have both positive and negative effects on the quality of cryopreserved spermatozoa [ 14 , 15 , 16 , 41 ]. The effect of SP proteins is very complex and has not been fully understood yet.…”

Section: Discussionmentioning

confidence: 99%

“…These processes result in changes in lipid composition and consequently in decreased PM fluidity and permeability [ 9 ], leading to premature capacitation-like and acrosome reaction-like changes in sperm [ 10 ]. Currently, in bulls, boars, and rams, it seems that the mentioned negative effects of cryopreservation can be reduced by adding a certain amount of SP or particular components thereof to the suspension (spermatozoa + extender) during processing [ 6 , 11 , 12 , 13 , 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Seminal Plasma Protein Fractions on Stallion Sperm Cryopreservation

Bubeníčková

Maňásková-Postlerová

Šimoník

et al. 2020

IJMS

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Seminal plasma (SP) is the natural environment for spermatozoa and contains a number of components, especially proteins important for successful sperm maturation and fertilization. Nevertheless, in standard frozen stallion insemination doses production, SP is completely removed and is replaced by a semen extender. In the present study, we analyzed the effects of the selected seminal plasma protein groups that might play an important role in reducing the detrimental effects on spermatozoa during the cryopreservation process. SP proteins were separated according to their ability to bind to heparin into heparin-binding (Hep+) and heparin-non-binding (Hep−) fractions. The addition of three concentrations—125, 250, and 500 µg/mL—of each protein fraction was tested. After thawing, the following parameters were assessed: sperm motility (by CASA), plasma membrane integrity (PI staining), and acrosomal membrane integrity (PNA staining) using flow cytometry, and capacitation status (anti-phosphotyrosine antibody) using imaging-based flow cytometry. Our results showed that SP protein fractions had a significant effect on the kinematic parameters of spermatozoa and on a proportion of their subpopulations. The 125 µg/mL of Hep+ protein fraction resulted in increased linearity (LIN) and straightness (STR), moreover, with the highest values of sperm velocities (VAP, VSL), also this group contained the highest proportion of the fast sperm subpopulation. In contrast, the highest percentage of slow subpopulation was in the groups with 500 µg/mL of Hep+ fraction and 250 µg/mL of Hep− fraction. Interestingly, acrosomal membrane integrity was also highest in the groups with Hep+ fraction in concentrations of 125 µg/mL. Our results showed that the addition of protein fractions did not significantly affect the plasma membrane integrity and capacitation status of stallion spermatozoa. Moreover, our results confirmed that the effect of SP proteins on the sperm functionality is concentration-dependent, as has been reported for other species. Our study significantly contributes to the lack of studies dealing with possible use of specific stallion SP fractions in the complex puzzle of the improvement of cryopreservation protocols. It is clear that improvement in this field still needs more outputs from future studies, which should be focused on the effect of individual SP proteins on other sperm functional parameters with further implication on the success of artificial insemination in in vivo conditions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Seminal Plasma Protein Fractions on Stallion Sperm Cryopreservation

Bubeníčková

Maňásková-Postlerová

Šimoník

et al. 2020

IJMS

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“…Zalazar et al [150] reported that addition of heterologous SPINK3 before or after freezing-thawing decreased protein tyrosine phosphorylation and intracellular calcium influx in ram sperm. However, other sperm parameters related to sperm quality and fertility only improved when this recombinant protein was added after cryopreservation, probably due to the interaction of SPINK3 with extender components such as egg yolk before freezing, which may alter the ability of this protein to reduce sperm cryodamage [151].…”

Section: Molecular Aspects Of Those Novel Strategies To Reduce Sperm mentioning

confidence: 99%

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Peris-Frau¹,

Soler²,

Iniesta‐Cuerda³

et al. 2020

IJMS

139

118

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Sperm cryopreservation represents a powerful tool for livestock breeding. Several efforts have been made to improve the efficiency of sperm cryopreservation in different ruminant species. However, a significant amount of sperm still suffers considerable cryodamage, which may affect sperm quality and fertility. Recently, the use of different “omics” technologies in sperm cryobiology, especially proteomics studies, has led to a better understanding of the molecular modifications induced by sperm cryopreservation, facilitating the identification of different freezability biomarkers and certain proteins that can be added before cryopreservation to enhance sperm cryosurvival. This review provides an updated overview of the molecular mechanisms involved in sperm cryodamage, which are in part responsible for the structural, functional and fertility changes observed in frozen–thawed ruminant sperm. Moreover, the molecular basis of those factors that can affect the sperm freezing resilience of different ruminant species is also discussed as well as the molecular aspects of those novel strategies that have been developed to reduce sperm cryodamage, including new cryoprotectants, antioxidants, proteins, nanoparticles and vitrification.

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“…Beyond the spermatozoa themselves, the complex, proteindominated, milieu comprising the seminal plasma has also received considerable attention it terms of its capacity to stabilize and reduce stress on the sperm membrane thereby preventing damage incurred during cryo-/liquid-preservation [56,[64][65][66] An important component of this milieu are the EVs that originate from accessory sex glands or upstream in the testis and epididymis. [28] The most widely studied of these EVs are prostasomes, which are secreted from the prostate epithelial cells and thereafter interact with spermatozoa to modulate their function and afford protection against the immune defences encountered within the female reproductive tract.…”

Section: Discussion Of Findingsmentioning

confidence: 99%

Proteomic analysis of koala (phascolarctos cinereus) spermatozoa and prostatic bodies

et al. 2021

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The aims of this study were to investigate the proteome of koala spermatozoa and that of the prostatic bodies with which they interact during ejaculation. For this purpose, spermatozoa and prostatic bodies were fractionated from the semen of four male koalas and analysed by HPLC MS/MS. This strategy identified 744 sperm and 1297 prostatic body proteins, which were subsequently attributed to 482 and 776 unique gene products, respectively. Gene ontology curation of the sperm proteome revealed an abundance of proteins mapping to the canonical sirtuin and 14-3-3 signalling pathways. By contrast, protein ubiquitination and unfolded protein response pathways dominated the equivalent analysis of proteins uniquely identified in prostatic bodies. Koala sperm proteins featured an enrichment of those mapping to the functional categories of cellular compromise/inflammatory response, whilst those of the prostatic body revealed an over-representation of molecular chaperone and stressrelated proteins. Cross-species comparisons demonstrated that the koala sperm proteome displays greater conservation with that of eutherians (human; 93%) as opposed to reptile (crocodile; 39%) and avian (rooster; 27%) spermatozoa. Together, this work contributes to our overall understanding of the core sperm proteome and has identified biomarkers that may contribute to the exceptional longevity of koala spermatozoa during ex vivo storage.

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Extenders modify the seminal plasma ability to minimize freeze‐thaw damage on ram sperm

Cited by 13 publications

References 49 publications

Effect of Seminal Plasma Protein Fractions on Stallion Sperm Cryopreservation

Effect of Seminal Plasma Protein Fractions on Stallion Sperm Cryopreservation

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Proteomic analysis of koala (phascolarctos cinereus) spermatozoa and prostatic bodies

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