Effect of Seminal Plasma Protein Fractions on Stallion Sperm Cryopreservation

Bubeníčková, Filipa; Maňásková-Postlerová, Pavla; Šimoník, Ondřej; Sirohi, Jitka; Šichtař, Jiří

doi:10.3390/ijms21176415

Cited by 18 publications

(12 citation statements)

References 78 publications

(128 reference statements)

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“…In the case of the fraction containing 50-100 kDa proteins, the effects were less clear, as the viability of bound-to-PMN sperm population was lower than both the control and the fraction containing 30-50 kDa proteins after 2 h and 3 h of incubation at 38 • C. Remarkably, viability of the unbound sperm population was the lowest in fractions containing small proteins (< 3 kDa and 3-10 kDa). Related to this, when they remain in contact with spermatozoa, low molecular proteins (i.e., <10 kDa) are not beneficial for sperm, but rather they reduce motility and damage plasma membrane integrity in several species [35][36][37][38][39]. The effect of these proteins could explain why the addition of seminal plasma semen does not improve frozen-thawed donkey sperm [5].…”

Section: Discussionmentioning

confidence: 99%

Specific Seminal Plasma Fractions Are Responsible for the Modulation of Sperm–PMN Binding in the Donkey

Miró

Catalán

Marín

et al. 2021

Animals

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While artificial insemination (AI) with frozen-thawed sperm results in low fertility rates in donkeys, the addition of seminal plasma, removed during cryopreservation, partially counteracts that reduction. Related to this, an apparent inflammatory reaction in jennies is induced following AI with frozen-thawed sperm, as a high amount of polymorphonuclear neutrophils (PMN) are observed within the donkey uterus six hours after AI. While PMN appear to select the sperm that ultimately reach the oviduct, two mechanisms, phagocytosis and NETosis, have been purported to be involved in that clearance. Remarkably, sperm interacts with PMN, but the presence of seminal plasma reduces that binding. As seminal plasma is a complex fluid made up of different molecules, including proteins, this study aimed to evaluate how different seminal plasma fractions, separated by molecular weight (<3, 3–10, 10–30, 30–50, 50–100, and >100 kDa), affect sperm–PMN binding. Sperm motility, viability, and sperm–PMN binding were evaluated after 0 h, 1 h, 2 h, 3 h, and 4 h of co-incubation at 38 °C. Two seminal plasma fractions, including 30–50 kDa or 50–100 kDa proteins, showed the highest sperm motility and viability. As viability of sperm not bound to PMN after 3 h of incubation was the highest in the presence of 30–50 and 50–100 kDa proteins, we suggest that both fractions are involved in the control of the jenny’s post-breeding inflammatory response. In conclusion, this study has shown for the first time that specific fractions rather than the entire seminal plasma modulate sperm–PMN binding within the donkey uterus. As several proteins suggested to be involved in the control of post-AI endometritis have a molecular weight between 30 and 100 kDa, further studies aimed at determining the identity of these molecules and evaluating their potential effect in vivo are much warranted.

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Section: Discussionmentioning

confidence: 99%

Specific Seminal Plasma Fractions Are Responsible for the Modulation of Sperm–PMN Binding in the Donkey

Miró

Catalán

Marín

et al. 2021

Animals

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“…Based on the insemination dose, a single Kacang goat ejaculate could be divided into 57 doses (0.38 mL volume) with 12 μ g IGF-I complex protein/mL SM-EY extender (T1). Bubenickova et al [ 55 ] reported a positive correlation between the concentration of IGF-I complex protein in horse seminal plasma and the concentration, morphology, and motility of sperm. In addition, IGF-I maintained ram sperm functions following chilled storage [ 56 ] by providing a stable energy supply for sperm metabolism [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

A 150 kDa Protein Derived from Bull Seminal Plasma Extended the Survival Time of Kacang Goat Sperm Stored at 5°C

Susilowati

Mustofa

Wurlina

et al. 2021

Veterinary Medicine International

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Artificial insemination has proven to be an effective method for increasing population size and genetic quality of Kacang goats. However, innovation is required to maintain the quality of Kacang goat semen in storage. This study aimed to examine the effects of supplementing the 150 kDa protein assumed as IGF-I complex derived from bull seminal plasma in skim milk-egg yolk extender on the quality of Kacang goat sperm stored at 5°C. Twelve ejaculates collected from three Kacang goats were divided into three groups. In the control group (T0), the ejaculates were extended with skim milk-egg yolk only. In the treatment groups (T1 and T2), the ejaculates were extended with skim milk-egg yolk supplemented with the IGF-I complex protein at 12 μg and 24 μg/100 mL, respectively. The extended semen was stored at 5°C, and the viability, motility, intactness of the plasma membrane, malondialdehyde concentration, and apoptotic sperm percentage were evaluated daily for five days. The results showed that the T1 was the most effective treatment for maintaining Kacang goat semen at a quality acceptable for artificial insemination over five days of storage at 5°C. However, the T0 and T2 groups retained acceptable qualities for only three days at 5°C. It could be concluded that supplementation of 12 μg of the 150 kDa protein derived from bull seminal plasma per 100 mL extender successfully extended the life span of Kacang goat sperm for five days.

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“…Spermatozoa motility was higher in sperm samples with a high concentration of seminal IGF-1, and higher concentrations of IGF-1 are associated with higher sperm motility [45]. In addition, the concentration of the IGF-1 complex protein in seminal plasma is positively correlated with the concentration, morphology, and motility of sperm [75]. However, in this study, a higher dose of IGF-1 (T2 group) showed higher motility and viability of Kacang buck semen than the T0 group, but sperm motility and viability of the T2 group were lower than those of the T1 group sperm (Table -2).…”

Section: )mentioning

confidence: 93%

Effect of insulin-like growth factor-1 complex of Simmental bull seminal plasma on post-thawed Kacang buck semen fertility

et al. 2021

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Background and Aim: Kacang buck sperm is cryosensitive due to the seminal plasma of semen itself. Meanwhile, bull seminal plasma contains the insulin-like growth factor-1 (IGF-1) complex, which is cryoprotective. The addition of the crude protein of Simmental bull seminal plasma increased the quality of post-thawed semen of Kacang buck. The study was conducted to determine the effects of Simmental bull seminal plasma with IGF-1 on the fertility of post-thawed Kacang buck semen. Materials and Methods: Buck semen was diluted in the following skim milk-egg yolk extender preparations: Without the addition of Simmental bull seminal plasma IGF-1 complex protein (T0); with the addition of 12-μg Simmental bull seminal plasma IGF-1 complex protein (T1); and with the addition of 24-μg Simmental bull seminal plasma IGF-1 complex protein (T2). The extended semen was packed in 0.25-mL straws and frozen. Post-thawed semen fertility was evaluated based on the following variables: Sperm motility, viability, intact plasma membrane (IPM), malondialdehyde (MDA) levels, capacitation status, and acrosome reaction. The difference in each variable among the groups was evaluated using analysis of variance, followed by Tukey's honestly significant difference test, at a 95% level of significance. Meanwhile, principal component analysis (PCA) was used to identify the principal component of semen fertility among the seven parameters. Results: The T1 group showed the highest sperm motility, viability, IPM, and percentage of incapacitated sperm and the lowest MDA levels, percentage of capacitated sperm, and acrosome reaction. PCA revealed that sperm motility had a moderate to very robust correlation with other variables and is the most crucial parameter, accounting for 80.79% of all variables. Conclusion: The IGF-1 complex in Simmental bull seminal plasma was useful for increasing the fertility of post-thawed Kacang buck semen, and sperm motility was the principal component of semen fertility.

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Effect of Seminal Plasma Protein Fractions on Stallion Sperm Cryopreservation

Cited by 18 publications

References 78 publications

Specific Seminal Plasma Fractions Are Responsible for the Modulation of Sperm–PMN Binding in the Donkey

Specific Seminal Plasma Fractions Are Responsible for the Modulation of Sperm–PMN Binding in the Donkey

A 150 kDa Protein Derived from Bull Seminal Plasma Extended the Survival Time of Kacang Goat Sperm Stored at 5°C

Effect of insulin-like growth factor-1 complex of Simmental bull seminal plasma on post-thawed Kacang buck semen fertility

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