Effect of Sperm Cryopreservation in Farm Animals Using Nanotechnology

Akhtar, Muhammad Faheem; Ma, Qingshan; Li, Yan; Chai, Wenqiong; Zhang, Zhenwei; Li, Liangliang; Wang, Changfa

doi:10.3390/ani12172277

Cited by 11 publications

(5 citation statements)

References 132 publications

(138 reference statements)

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“…However, a noticeable trend towards increased membrane integrity was noted especially at 1-2% lycopene concentrations. This observation aligns with the findings of Akhtar et al (2010Akhtar et al ( , 2022, who reported that the membrane integrity of stallion and buffalo sperm showed no significant difference following cryopreservation…”

Section: Advances In Animal Andsupporting

confidence: 92%

Frozen Semen Quality of Kalang Buffalo Using Lycopene

Elvania,

Rostini,

Sakiman

et al. 2024

AAVS

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An important factor to successful of artificial insemination (AI) in buffalo was the quality of spermatozoa post-thawed. Therefore, research was conducted on the use of lycopene as an effort to improve the quality of spermatozoa post-thawing. This study aimed to assess the impact of incorporating lycopene into both the skim milk and egg yolk extender at varying doses on pre-freeze and post-thawed Kalang buffalo semen. Four Kalang buffalo, aged between 6 to 7 years and weighing 500-550 kg, were involved in this investigation. Semen collection was conducted weekly a 12-week period using an artificial vagina. Lycopene was added to the skim milk-egg yolk diluent at concentrations of 1%, 2%, 3%, and 4%, while the control group received no lycopene substitution. The freshly collected semen underwent macroscopic and microscopic evaluations. Subsequently, the semen was assessed pre-freezing and post-thaw with parameters such as viability, motility, abnormality, and plasma membrane integrity. The findings revealed that the inclusion of 1% and 2% lycopene in the diluent before freezing exhibited significantly higher (P < 0.05) spermatozoa motility. Moreover, the viability and motility of post-thawed semen significantly improved (P < 0.05) at lycopene doses of 1% and 2%. The addition of 1% to 2% lycopene in the extender demonstrated a positive effect on the sperm qualities, particularly enhancing the viability and motility of post-thaw semen from Kalang buffalo.

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Section: Advances In Animal Andsupporting

confidence: 92%

Frozen Semen Quality of Kalang Buffalo Using Lycopene

Elvania,

Rostini,

Sakiman

et al. 2024

AAVS

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“…In this work, double IP, cytoplasmatic droplets and head detachment were considered as SPZ IPD. Several studies have confirmed that sometimes it is difficult to identify which factors mainly affects frozen semen quality [43,44]. In fact, there are several factors inherent to the animal and many environmental factors as well, such as temperature, management or different SPZ cryopreservation protocols [27,29,33].…”

Section: Discussionmentioning

confidence: 99%

Ram Semen Cryopreservation for Portuguese Native Breeds: Season and Breed Effects on Semen Quality Variation

Barbas

Pimenta

Baptista

et al. 2023

Animals

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The semen quality is one of the determinant factors of ram semen cryopreservation. The present retrospective study aimed to characterize the seasonal ram pattern during the year for ten Portuguese local sheep breeds, hypothesizing that the breed and season had low effects on the main spermatozoa traits. A total of 1471 ejaculates were used and evaluated (fresh semen) from 85 rams between 2004 and 2020 and re-evaluated after thawing (thawed semen). The effect of breed, season, and sperm cryopreservation on nine semen traits were evaluated. The volume per ejaculate, spermatozoa (SPZ) concentration, and total number of SPZ per ejaculate, were affected by breed (p < 0.001) but not by season (p > 0.05). As expected, the semen processing was the most significant (p < 0.001) factor of variation on seminal parameters. Moreover, breed and interactions between breed × semen processing, modulated the response of alive SPZ, abnormal morphology, head, and intermediate piece defects. In fresh semen, season only affected the intermediate piece defects due to the highest percentage observed between February and April period in some breeds. Overall, and despite the mentioned particularities, there were similarities among the ten local breeds. We also concluded that the seasonal effect on ejaculate and SPZ traits is not significant in our region. These local ram breeds have low seasonality and can be employed in natural mating as well as semen donors for cryopreservation and assisted reproductive biotechnologies during the whole year at our latitude.

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“…When the amount of ROS in the body goes up, it damages the DNA of sperm and makes it harder for them to fertilize eggs. During cryopreservation, ROS are made, which changes the sperm's mitochondrial membrane potential (6). Cryopreservation changes how sperm membrane lipids are made, how long they live, and how they move.…”

Section: Discussionmentioning

confidence: 99%

Mitokondri hedefli bir antioksidan olan Mito-TEMPO’nun donmuş insan sperm parametrelerine etkisi

SAYLAN¹,

Dıramali²

2023

tjhealthsport

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Aim:The goal of this study is to find out if Mito-TEMPO, an antioxidant that targets mitochondria, can improve the quality of frozen human sperm.Material-Method: Normospermic 25 human semen samples were frozen and stored in solutions including different concentrations (0, 5, 10 and 50 µM) of Mito-TEMPO. After thawing, they were evaluated in terms of sperm motility, viability, morphology, chromatin integrity and apoptosis.Result: Sperm morphology was not found to change after cryopreservation. Viability was found to be significantly preserved in groups that were added Mito-TEMPO (p<0.01). When the motility of the sperms after they had been frozen was compared, the group that added 50 µM Mito-TEMPO had the most motile sperms. (p<0.05), even though there was no statistically significant difference between the groups that added 1 µM Mito-TEMPO and the groups that did not add Mito-TEMPO. In addition, chromatin decondensation and apoptosis rate decreased significantly in groups that were added Mito-TEMPO (p<0.01). Conclusion:Mitochondria targeted antioxidant Mito-TEMPO improves sperm quality that decreases after thawing.

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Effect of Sperm Cryopreservation in Farm Animals Using Nanotechnology

Cited by 11 publications

References 132 publications

Frozen Semen Quality of Kalang Buffalo Using Lycopene

Frozen Semen Quality of Kalang Buffalo Using Lycopene

Ram Semen Cryopreservation for Portuguese Native Breeds: Season and Breed Effects on Semen Quality Variation

Mitokondri hedefli bir antioksidan olan Mito-TEMPO’nun donmuş insan sperm parametrelerine etkisi

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