Larvicidal Constituents ofZingiber officinale(Ginger) againstAnisakis simplex

Abstract

In this study, we investigated the anthelmintic activity of [10]-shogaol, [6]-shogaol, [10]-gingerol and [6]-gingerol, compounds isolated from the roots of Zingiber officinale L., Zingiberaceae (ginger), against Anisakis simplex. The above compounds kill or reduce spontaneous movement in A. simplex larvae. The maximum lethal efficacy of [10]-shogaol and [10]-gingerol was approximately 80% and 100%, respectively. We further examined the time course of compound-induced loss of mobility in A. simplex. The results showed that various concentrations of [10]-shogaol, [6]-shogaol, [10]-gingerol and [6]-gingerol have maximum effects on loss of spontaneous movement from 24 to 72 h. In addition, the time course of mortality and the percentage of loss of spontaneous movements were ascertained to determine the minimum effective doses of [10]-gingerol and [10]-shogaol. [10]-Gingerol exhibited a larger maximum larvicidal effect and greater loss of spontaneous movement than [10]-shogaol and albendazole. In addition, these constituents of Zingiber officinale showed effects against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and peroxyl radicals. These constituents of Zingiber officinale are responsible for its larvicidal activity against A. simplex.

10-Shogaol, an Antioxidant from Zingiber officinale for Skin Cell Proliferation and Migration Enhancer

“…Dried and chipped rhizomes (25.6 kg) of Z. officinale were extracted repeatedly with different mixtures of chloroform (50 L × 4) at room temperature, based on previous reports [31]. The combined chloroform extracts (896.5 g) were then evaporated further and separated into 20 fractions by column chromatography (CC) in the silica gel (3.8 kg, 70–230 mesh) with gradients of n -hexane/CHCl 3 .…”

Natural products: Perspectives in the pharmacological treatment of gastrointestinal anisakiasis

“…Moreover, Lin et al [31] Suzuki et al [32] studied the effect of other compounds of natural origin. Of these, (+)-ar-turmerone, one of the main components of essential oil from the rhizomes of Curcuma longa (C. longa), was the most effective (25 mg/mL) at the 24-hour point.…”

Antischistosomal activity of ginger (Zingiber officinale) against Schistosoma mansoni harbored in C57 mice

“…Lin et al (2010a) suggested that the 6-gingerol, 10-shogaol, 10-gingerol, 6-shogaol and hexahydrocurcumin, a constituent isolated from the ginger might be used as larvicidal agents against Angiostrongylus cantonensis. Lin et al (2010b) investigated the anthelmintic activity of previous compounds against the nematode worm Anisakis simplex; they reported that these compounds kill or reduce spontaneous movement in A. simplex larvae. Concerned with schistosomiasis, the bioactivity of an ethyl acetate extract of ginger (Z. officinale) towards Schistosoma mansoni adult pairs, both cultured in vitro and in vivo in laboratory mice, was investigated.…”