Large-scale use of liquid-phase blocking sandwich ELISA for the evaluation of protective immunity against aphthovirus in cattle vaccinated with oil-adjuvanted vaccines in Argentina

Periolo, Osvaldo; Seki, Cristina; Grigera, Pablo R.; Robiolo, Blanca; Fernandez, G.; Maradei, Eduardo; D’Aloia, Ricardo; Torre, J.L. La

doi:10.1016/0264-410x(93)90261-u

Cited by 41 publications

(13 citation statements)

References 14 publications

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“…FMDV-specific total antibody titers were determined by an lpELISA originally developed by Hamblin et al (26) and further modified by Periolo et al (27).…”

Section: Methodsmentioning

confidence: 99%

Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle

Pega

Bucafusco

Giacomo

et al. 2013

J Virol

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Foot-and-mouth disease (FMD) is a highly contagious viral disease which affects both domestic and wild biungulate species. This acute disease, caused by the FMD virus (FMDV), usually includes an active replication phase in the respiratory tract for up to 72 h postinfection, followed by hematogenous dissemination and vesicular lesions at oral and foot epithelia. The role of the early local adaptive immunity of the host in the outcome of the infection is not well understood. Here we report the kinetics of appearance of FMDV-specific antibody-secreting cells (ASC) in lymphoid organs along the respiratory tract and the spleen in cattle infected by aerosol exposure. While no responses were observed for up to 3 days postinfection (dpi), all animals developed FMDV-ASC in all the lymphoid organs studied at 4 dpi. Tracheobronchial lymph nodes were the most reactive organs at this time, and IgM was the predominant isotype, followed by IgG1. Numbers of FMDV-ASC were further augmented at 5 and 6 dpi, with an increasing prevalence in upper respiratory organs. Systemic antibody responses were slightly delayed compared with the local reaction. Also, IgM was the dominant isotype in serum at 5 dpi, coinciding with a sharp decrease of viral RNA detection in peripheral blood. These results indicate that following aerogenous administration, cattle develop a rapid and vigorous genuine local antibody response throughout the respiratory tract. Time course and isotype profiles indicate the presence of an efficient T cell-independent antibody response which drives the IgM-mediated virus clearance in cattle infected by FMDV aerosol exposure.

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“…FMDV-specific total antibody titers were determined by an lpELISA originally developed by Hamblin et al (26) and further modified by Periolo et al (27).…”

Section: Methodsmentioning

confidence: 99%

Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle

Pega

Bucafusco

Giacomo

et al. 2013

J Virol

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“…Maximum antibody titres are typically reached 7-10 days post-vaccination, although protective titres may be reached after only 2-3 days post-vaccination. Cattle are considered protected from foot-and-mouth disease when antibody titres exceed 100 (as measured by Liquid Phase Blocking ELISA), although this may vary with the severity of challenge [11,12] Acrit-log r (where r is degree of homology) are protected (immune) and those with log antibody titres below the threshold are susceptible. Log antibody titre decays linearly through time at the rate y so mean log antibody titre at time t is given by A(t) = Aint-Yt.…”

Section: Vaccination Trial Datamentioning

confidence: 99%

Failure of vaccination to prevent outbreaks of foot-and-mouth disease

Woolhouse

Haydon

Pearson³

et al. 1996

Epidemiol. Infect.

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SUMMARYOutbreaks of foot-and-mouth disease persist in dairy cattle herds in Saudi Arabia despite revaccination at intervals of 4-6 months. Vaccine trials provide data on antibody responses following vaccination. Using this information we developed a mathematical model of the decay of protective antibodies with which we estimated the fraction of susceptible animals at a given time after vaccination. The model describes the data well, suggesting over 95 % take with an antibody half-life of 43 days. Farm records provided data on the time course of five outbreaks. We applied a 'SLIR' epidemiological model to these data, fitting a single parameter representing disease transmission rate. The analysis provides estimates of the basic reproduction number, R, which may exceed 70 in some cases. We conclude that the critical intervaccination interval which would provide herd immunity against FMDV is unrealistically short, especially for heterologous challenge. We suggest that it may not be possible to prevent foot-and-mouth disease outbreaks on these farms using currently available vaccines.

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“…This findings support the finding of Madhanmohan et al (2009), who stated that MDA titers are likely to persist for 4-5 months of age in calves. Periolo et al (1993) and Auge de Mello et al (1989) also opined that young calves are the mandatory candidate for FMD vaccination at 4-5 months after birth when they lose passive immunity. Irrespective of vaccine types, male calves had slightly higher level of MDA than that of female calves and PI values were comparatively higher against serotype O than the other two serotypes (A and Asia 1).…”

Section: Resultsmentioning

confidence: 99%

Persistence of Maternally Derived Antibodies in Calves to Vaccination Against Foot and Mouth Disease

Akhter

Ms²,

Uddin³

et al. 2016

Bangl. J. Vet. Med.

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This study aimed to know the level of maternally derived antibody (MDA) titer in calves born to cows vaccinated with inactivated trivalent (type O, A and Asia 1) Foot and Mouth disease vaccine. Three groups of calves (10 calves in each group) of different age were randomly selected from two organized farms from Savar Upazilla of Bangladesh where the dams are routinely vaccinated with trivalent FMD vaccines. Blood samples were collected from these selected calves in six occasions five weeks apart and sera (n=180) were tested for antibody titer against FMDV by using liquid phase blocking ELISA (LPB-ELISA) test. The test is based upon specific blocking of the FMDV antigen in liquid phase by antibodies in the test serum sample. Protective level (PI value >50) of maternal antibody against FMDV was found in serum of calves up to the age of 22-23 wks (above five months) and decreased below protective level (PI value <50) at 27-28 weeks (above 6 months) of age. Irrespective of vaccine types, male calves had slightly higher level of MDA than that of female calves and PI values were comparatively higher against serotype O than the other two serotypes (A and Asia 1). Calves born to vaccinated dams could be vaccinated for first time after five months of their birth to lower the incidence of foot and mouth disease.

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Large-scale use of liquid-phase blocking sandwich ELISA for the evaluation of protective immunity against aphthovirus in cattle vaccinated with oil-adjuvanted vaccines in Argentina

Cited by 41 publications

References 14 publications

Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle

Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle

Failure of vaccination to prevent outbreaks of foot-and-mouth disease

Persistence of Maternally Derived Antibodies in Calves to Vaccination Against Foot and Mouth Disease

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