Large-scale, high-efficiency production of coffee somatic embryos

Maciel, Anna Lygia de Rezende; Rodrigues, Filipe Almendagna; Pasqual, Moacir; Carvalho, C. H. S.

doi:10.1590/1984-70332016v16n2a16

Cited by 10 publications

(6 citation statements)

References 16 publications

(19 reference statements)

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“…Auxin and cytokinin combinations significantly affected callus induction in both Code 93 and Code 71. Similar observations have indeed been made in other studies including Gatica-Arias et al (2008), Maciel et al (2016) and Aga and Khillare (2017) in C. arabica callus induction using auxin and cytokinin combinations.…”

Section: Callus Inductionsupporting

confidence: 91%

“…The PGR used singly, auxin 2,4-D induced callus in all treatments in Code 71 and Code 93. This agrees with and reemphasize the observations by Maciel et al, (2016) in Coffea arabica L., Rashmi and Trivedi (2014), in Nerium odorum (Apocynaceae), Dos Santos and De Souza (2016) in Capsicum annum, Gopitha et al (2010) in Saccharum officinarum (Sugarcane), and Lee et al (2011) in Morus alba (Mulberry) all whom observed embryogenic response in callus formation using 2,4-D.…”

Section: Callus Inductionsupporting

confidence: 88%

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Effects of genotype and plant growth regulators on callus induction in leaf cultures of Coffea arabica L. F1 hybrid

Mwaniki¹,

Lubabali²,

Asava³

et al. 2019

Afr. J. Biotechnol.

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Coffea arabica, F1 hybrid variety, Ruiru 11 is a highly sought after crop in Kenya due to its alleviated resistance to Coffee Berry Disease and Coffee Leaf Rust coupled with high yield capacity and good cup quality. Access to the variety's planting materials is limited due to challenges with difficulty in propagation using conventional methods of seed and vegetative propagation; and somatic embryogenesis is regarded as a suitable alternative propagation method. Therefore, the current study aimed to establish an induction protocol in F1 composite hybrid Ruiru 11. The current study investigated the effects of genotype and plant growth regulators, auxins and cytokinins, on induction of embryogenic callus in two composite genotypes of C. arabica L. F1 hybrid variety Ruiru 11. Leaf explants from the F1 hybrid were cultured on half-strength Murashige and Skoog (MS) media supplemented with varied concentrations and combinations of plant growth regulators. Callus formation was evaluated weekly until the 60 th day. Genotypic effects were assessed based on the difference on callus induction rates, biomass fresh weights and callus formation. The genotypes tested showed highest callus induction 88% (Code 71) and 100% (Code 93) with respect to the formation of embryogenic calli. Highest fresh weight was obtained at 0.973 ± 0.011 g in Code 71 and 0.649 ± 0.03 g in Code 93 in MS media supplemented with 2,4-D + BAP (0.53 + 0.11 µM). The observed results are useful in formulating the best growth regulator concentration suitable for mass in vitro propagation genotypes of Arabica coffee hybrid Ruiru 11 through callus induction in vitro of leaf explants.

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Section: Callus Inductionsupporting

confidence: 91%

Section: Callus Inductionsupporting

confidence: 88%

Effects of genotype and plant growth regulators on callus induction in leaf cultures of Coffea arabica L. F1 hybrid

Mwaniki¹,

Lubabali²,

Asava³

et al. 2019

Afr. J. Biotechnol.

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“…Despite the higher production cost, aggregated value of clonal seedlings resistant to RKN may compensate farmer's investment in infested areas. To address this question, several works have been conducted to increase efficiency of somatic embryogenesis protocols and to reduce production costs of clonal seedlings (Etienne et al, 2012;Etienne et al, 2013;Maciel et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Thus, clonal propagation of heterozygous genotypes of C. arabica represents an alternative for large-scale multiplication of plants that segregate for the characteristic of interest. Somatic embryogenesis is a valuable alternative to propagate high yielding F 1 hybrids (Bertrand et al, 2011) and plants resistant to leaf miner Leucoptera coffeella Guérin-Méneville and leaf rust Hemileia vastatrix Berkeley and Broome (Andreazi et al, 2015;Maciel, 2016;Mendonça et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Clonal Arabica coffee resistant to Meloidogyne paranaensis and damage threshold on plants development

Fatobene

Gonçalves

Oliveira

et al. 2019

Sci. agric. (Piracicaba, Braz.)

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Plants of Coffea arabica Icatu IAC 925 germplasm show resistance to Meloidogyne paranaensis under field conditions, but with variable rates of segregation. One mother plant, IAC 3, of Icatu IAC 925 was evaluated under controlled conditions through the reaction of its clones, obtained by somatic embryogenesis. Reproduction of nematodes in clones were compared with an open-pollinated progeny from the same mother plant, C. arabica cultivar Mundo Novo IAC 515-20, susceptible to nematodes, and Clone IAC 1, also susceptible and obtained by somatic embryogenesis. Open-pollinated progeny showed segregation to resistance and was classified as susceptible according to the reproduction factor. The average number of nematodes (eggs and J2) per gram of roots in clones was eight fold lower than that of open-pollinated progeny,and 51 fold lower than Mundo Novo IAC 515-20. Based on Seinhorst model, IAC 3 clone showed a tolerance limit of 7.7 nematodes per cm 3 of soil, while in Mundo Novo the tolerance limit was 3.9 nematodes per cm 3 of soil. These results revealed that clonal Arabica coffee nematoderesistant trees represent an alternative to the time consuming and expensive process inherent to conventional breeding strategies.

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“…Bioreactor technology is a mechanized system used in plant tissue culture to improve the large-scale micropropagation in laboratories and biofactories. Therefore, bioreactor comes as an alternative technological tool to the conventional micropropagation process aimed at optimizing the in vitro cultivation by the automation of the process (Maciel et al 2016, Mariateresa et al 2014, Oliveira et al 2011b). These mechanized systems can help achieve a higher plant biomass gain and reduce the time needed for propagation (Máximo et al 2015).…”

Section: Introductionmentioning

confidence: 99%

In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects

Máximo

Santos

Martins

et al. 2018

Crop Breed. Appl. Biotechnol.

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This study aimed to assess the influence of culture medium and cytokinin type on in vitro multiplication of an Eucalyptus grandis x E. urophylla hybrid clone via temporary immersion bioreactor (TIB ®). JADS, modified MS at a (NO 3ˉ) :(NH 4 +) ratio, and WPM were used as liquid media; and BAP, KIN, and TDZ were the cytokinins used in the study. According to the results, explants under the influence of modified MS presented better results for all traits. BAP was the most suitable plant growth regulator for axillary bud shoot proliferation. By the leaf histological analysis, most of the shoots grown in the presence of BAP either exhibited normal morphology or only a few hyperhydricity symptoms. All these results were obtained at 19 days after in vitro cultivation, a shorter period when compared with other works in the literature, showing the protocol efficiency in the multiplication of this hybrid clone using TIB ® .

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Large-scale, high-efficiency production of coffee somatic embryos

Cited by 10 publications

References 16 publications

Effects of genotype and plant growth regulators on callus induction in leaf cultures of Coffea arabica L. F1 hybrid

Effects of genotype and plant growth regulators on callus induction in leaf cultures of Coffea arabica L. F1 hybrid

Clonal Arabica coffee resistant to Meloidogyne paranaensis and damage threshold on plants development

In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects

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