In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects

Máximo, Wesley Pires Flausino; Santos, Paulo Augusto Almeida; Martins, Guilherme Silva; Mendonça, Evânia Galvão; Paiva, Luciano Vilela

doi:10.1590/1984-70332018v18n2a19

Cited by 10 publications

(10 citation statements)

References 22 publications

(28 reference statements)

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“…Nevertheless, a standard solid medium multiplication protocol cannot simply be copy/pasted to a liquid medium system. Problems to be solved are small shoots, hyperhydricity, excessive leaf drop and break out of endogenous bacterial contamination (Máximo et al, 2018).…”

Section: Microcutting and Rootingmentioning

confidence: 99%

Somatic Embryogenesis as Key Technology for Shaping the Rubber Tree of the Future

Mignon¹,

Werbrouck

2018

Front. Plant Sci.

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Worldwide, Hevea producers face the need to replant large surfaces in the coming years. The rubber yield per ha, produced by trees grafted on heterogeneous illegitimate seedling rootstocks, has reached its maximum. For long-standing Hevea clones, as for a lot of other tree species, one of the consequences of physiological aging is reduced in vitro growth and the lack of a proper geotropic (tap) root system. Somatic embryogenesis on young inner seed integument or stamen filaments provides a mean to regain ontogenetic juvenility. The process is limited by irregular germination of the somatic embryos. Nevertheless, with the obtained in vitro plants, juvenile lines have been established of the most important profitable rubber tree clones. Currently they are micropropagated on a commercial scale. Moreover, the produced plants can serve as mother plants for propagation by means of macro-cutting. Somatic embryogenesis enables the production of transgenic Hevea brasiliensis as well. Genes conferring plant disease resistance, abiotic stress tolerance and production of foreign proteins in the lactiferous vessels will further shape the rubber tree of the future.

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Section: Microcutting and Rootingmentioning

confidence: 99%

Somatic Embryogenesis as Key Technology for Shaping the Rubber Tree of the Future

Mignon¹,

Werbrouck

2018

Front. Plant Sci.

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“…No que se refere a borracha natural proveniente da H. brasiliensis, é possível afirmar que as suas propriedades biopolímeras são únicas e não podem ser replicadas por alternativas sintéticas, o que a torna matéria-prima insubstituível para muitas aplicações. Quando comparada com a borracha sintética, a borracha natural apresenta benefícios como proporcionar uma baixa geração de calor sob atrito, alta resistência a rupturas, boa resistência a abrasão, além das suas características elásticas e natureza à prova d'água, que são consideradas excelentes para aplicação em composições de pneus, material bélico, transporte e outros (Zwart, Krabbenborg, & Zwier, 2015;Men, Wang, Chen, Zhang, & Xian, 2019). Devido à crescente demanda por borracha natural, a cultura de tecidos e a genética são técnicas promissoras que podem ser usadas na seleção precoce para o melhoramento em Hevea e produção em larga escala (Venkatachalam, Geetha, Sangeetha, & Thulaseedharan, 2013).…”

Section: Introductionunclassified

“…Alguns avanços foram obtidos recentemente na micropropagação de H. brasiliensis, onde se obteve plântulas in vitro com sistemas radicular e aéreo desenvolvidos, porém estas plântulas estagnaram e morreram ainda no meio de cultura (Ighere, Anthony;Olayode, & Sunday, 2011;Antwi-Wiredu, Amiteye, Diawuoh, Asumeng, & Klu, 2018). As principais limitações a serem resolvidas para esta espécie são hiperidricidade, oxidação e contaminação endógena (Máximo, Santos, Martins, Mendonça, & Paiva, 2018).…”

Section: Introductionunclassified

Indução da calogênese em segmentos foliares de seringueira (Hevea spp.) na Amazônia Sul-Ocidental

Silva

Manfio

Leão

et al. 2021

RSD

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Mesmo considerando os avanços nos estudos de micropropagação em Hevea spp., a compreensão dos estímulos e condições necessárias para o estabelecimento do cultivo in vitro ainda são limitadas. Uma das razões para isto acontecer é devido à falta de um protocolo eficiente para a propagação de clones elites de seringueira em larga escala. Neste sentido, investigamos o efeito do ácido 2,4-diclorofenoxiacético na indução de calos em segmentos foliares de Hevea spp., bem como o uso do PPM® (Plant Preservative Mixture) na inibição de contaminações. Folhas juvenis de seringueira foram inoculadas em meio de cultura MS (Murashig Skoog) suplementado com 2,4-D (0; 0,25; 0,5; 1,0; 2,0 e 4,0 mg L-1) com biocida PPM® (0 e 1,0 mL L-1). Após 60 dias foi possível observar maior porcentagem de sobrevivência e expressiva oxidação fenólica na presença do PPM®. As concentrações de 2,4-D (2,0 e 4,0 mg L-1) combinadas com PPM® (1 mL L-1) foram eficientes na inibição de contaminações microbianas. A indução de calo ocorreu no tratamento contendo 1 mg L-1 de 2,4-D e 1 mL L-1 de PPM® e formou um calo compacto com coloração branca. O protocolo utilizado neste trabalho foi eficiente para desinfestação dos explantes foliares de Hevea spp.

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“…Both bioreactor systems offer increased culture multiplication and high biomass accumulation due to high nutrient availability. Compared to traditional cultivation in a liquid medium, bioreactors offer reduced hyperhydricity and physiological stress [ 5 ], as well as lower medium costs (i.e., elimination of gelling agent) and shorter plant propagation time. They enable easier handling and reduce labor coast [ 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Transformed Shoots of Dracocephalum forrestii W.W. Smith from Different Bioreactor Systems as a Rich Source of Natural Phenolic Compounds

et al. 2020

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Transformed shoots of the Tibetan medicinal plant Dracocephalum forrestii were cultured in temporary immersion bioreactors (RITA and Plantform) and in nutrient sprinkle bioreactor (NSB) for 3 weeks in MS (Murashige and Skoog) liquid medium with 0.5 mg/L BPA (N-benzyl-9-(2-tetrahydropyranyl)-adenine) and 0.2 mg/L IAA (indole-3-acetic acid). The greatest biomass growth index (GI = 52.06 fresh weight (FW) and 55.67 dry weight (DW)) was observed for shoots in the RITA bioreactor, while the highest multiplication rate was found in the NSB (838 shoots per bioreactor). The levels of three phenolic acids and five flavonoid derivatives in the shoot hydromethanolic extract were evaluated using UHPLC (ultra-high performance liquid chromatography). The predominant metabolite was rosmarinic acid (RA)—the highest RA level (18.35 mg/g DW) and total evaluated phenol content (24.15 mg/g DW) were observed in shoots grown in NSB. The NSB culture, i.e., the most productive one, was evaluated for its antioxidant activity on the basis of reduction of ferric ions (ferric reducing antioxidant power, FRAP) and two scavenging radical (O2•– and DPPH, 1,1-diphenyl-2-picrylhydrazyl radical) assays; its antibacterial, antifungal, and antiproliative potential against L929 cells was also tested (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) test). The plant material revealed moderate antioxidant and antimicrobial activities and demonstrated high safety in the MTT test—no cytotoxicity at concentrations up to 50 mg/mL was found, and less than a 20% decrease in L929 cell viability was observed at this concentration.

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In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects

Cited by 10 publications

References 22 publications

Somatic Embryogenesis as Key Technology for Shaping the Rubber Tree of the Future

Somatic Embryogenesis as Key Technology for Shaping the Rubber Tree of the Future

Indução da calogênese em segmentos foliares de seringueira (Hevea spp.) na Amazônia Sul-Ocidental

Transformed Shoots of Dracocephalum forrestii W.W. Smith from Different Bioreactor Systems as a Rich Source of Natural Phenolic Compounds

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