Lanthanide‐Coordinated Semiconducting Polymer Dots Used for Flow Cytometry and Mass Cytometry

Abstract

Simultaneous monitoring of biomarkers as well as single-cell analyses based on flow cytometry and mass cytometry are important for investigations of disease mechanisms, drug discovery, and signaling-network studies. Flow cytometry and mass cytometry are complementary to each other; however, probes that can satisfy all the requirements for these two advanced technologies are limited. In this study, we report a probe of lanthanide-coordinated semiconducting polymer dots (Pdots), which possess fluorescence and mass signals. We demonstrated the usage of this dual-functionality probe for both flow cytometry and mass cytometry in a mimetic cell mixture and human peripheral blood mononuclear cells as model systems. The probes not only offer high fluorescence signal for use in flow cytometry, but also show better performance in mass cytometry than the commercially available counterparts.

Dual resonance energy transfer in triple-component polymer dots to enhance electrochemiluminescence for highly sensitive bioanalysis

“…Owing to their good biocompatibility, low cellular toxicity, easy functionalization, high photostability, and size/surface-trap controlled luminescence, polymer dots (Pdots) have been widely applied as promising fluorescent bioprobes in single particle tracing, biosensing and cellular labeling 1–6. The electrochemiluminescence (ECL) of Pdots has also attracted considerable attention due to their tunable optical and electrochemical properties 7–10.…”

A BODIPY‐Based Donor/Donor–Acceptor System: Towards Highly Efficient Long‐Wavelength‐Excitable Near‐IR Polymer Dots with Narrow and Strong Absorption Features

“…On the other hand, simultaneous identification of numerous biological species in a single experiment are important to minimize the sample consumption and running times for cell research and disease diagnosis and treatment. LWE‐Pdots with narrow absorption and emission bands are therefore expected to be highly useful in applications involving multi‐wavelength‐excitation‐based in vitro assays such as cell barcoding, flow cytometry, and quantitative polymerase chain reaction …”

Quantification of cyclic DNA polymerization with lanthanide coordination nanomaterials for liquid biopsy

“…21 Recently, lanthanide coordination polymers (Ln-CPs) have been developed to address the above issue. 22,23 In Ln-CPs, Ln elements are protected by coordination ligands against quenching by solvents. Typically, organic ligands are employed to synthesize Ln-CPs and induce the enhancement of uorescence by the so-called antenna effect.…”