Lafutidine inhibits <i>Helicobacter pylori</i>‐induced interleukin‐8 production in human gastric epithelial cells

Nozawa, Yoshihisa; Nishihara, Katsushi; Akizawa, Yushiro; Orimoto, Naoki; Nakano, Masaya; Uji, Tatsuya; Ajioka, Hirofusa; Kanda, Atsuhiro; Matsuura, Naosuke; Kiniwa, Mamoru

doi:10.1111/j.1440-1746.2003.03330.x

Cited by 8 publications

(8 citation statements)

References 23 publications

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“…These recruited immune cells can continually act on the epithehal cells, thereby stimulating the release of cytokines (Ismail et al, 2003;Nozawa et al, 2004). If the pathogenic bacteria-induced inflammatory response is not well controlled, it will result in tissue damage.…”

Section: Discussionmentioning

confidence: 99%

Reduced lesions in chickens with Clostridium perfringens-induced necrotic enteritis by Lactobacillus fermentum 1.2029 ,

Cao

Yang

et al. 2012

Poultry Science

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Previous studies have demonstrated that Lactobacillus has anti-inflammatory properties, but the protective functions of Lactobacillus and mechanisms of inhibition of necrotic enteritis (NE) in the intestines of chickens have not been fully clarified. In the present study, we selected a probiotic strain, Lactobacillus fermentum 1.2029, which has good adhesive ability and a high survival rate in low pH and bile salts. The objective of this study was to examine the anti-inflammatory properties of L. fermentum 1.2029 against NE in chickens. Two hundred forty 1-d-old male Arbor Acres broilers were blocked into 3 experimental groups as follows: (I) nonchallenge control group, (II) Clostridium perfringens challenge group, and (III) C. perfringens challenge + L. fermentum 1.2029 group. Lactobacillus fermentum 1.2029 (1.0 mL/d, 10(8) cfu/mL) was orally administered daily to group III during the course of the experiment, and all uninfected control chickens were inoculated accordingly with the same volume of PBS. Clostridium perfringens (0.5 mL on d 1 and 1.0 mL on d 14 to 21, 10(8) cfu/mL) was administered to chickens in group II. At 28 d, scoring of gross NE lesions was performed. Ileal segments of approximately 2 cm from 24 chickens in each experimental group were collected and fixed in 4% (wt/vol) neutral-buffered formalin solution for histological scoring. Ileal mucosa samples were also collected for mRNA analysis by real-time PCR. The results showed that L. fermentum 1.2029 reduced the severity of NE lesions in chickens. Histological scores revealed that L. fermentum 1.2029 also reduced the inflammation damage of NE in chickens. Changes in cytokines and Toll-like receptors (TLR) were determined, and L. fermentum 1.2029 was found to increase interleukin-10 levels and reduce interferon-γ and TLR2 levels in NE-infected chickens. The results showed that L. fermentum 1.2029 was able to regulate the intestinal mucosal immune response and ameliorate inflammation by changing expression levels of cytokines and TLR.

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Section: Discussionmentioning

confidence: 99%

Reduced lesions in chickens with Clostridium perfringens-induced necrotic enteritis by Lactobacillus fermentum 1.2029 ,

Cao

Yang

et al. 2012

Poultry Science

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“…Some investigators have shown that H2RA reduced the prevalence of gastroduodenitits in non‐CD patients 32, 33 . A recent in vitro study confirmed that laftidine, one of H2RAs, inhibits H. pylori induced IL‐8 production in human gastric epithelial cells 34 . However, the natural course and the response to medications of diminutive gastroduodenal lesions of CD have not been investigated previously.…”

Section: Discussionmentioning

confidence: 99%

“…32,33 A recent in vitro study confirmed that laftidine, one of H2RAs, inhibits H. pylori induced IL-8 production in human gastric epithelial cells. 34 However, the natural course and the response to medications of diminutive gastroduodenal lesions of CD have not been investigated previously. In the present investigation, there were trends towards decreases in IL-1b and IL-8 in cardial mucosa after administration of famotidine.…”

Section: Discussionmentioning

confidence: 99%

Mucosal proinflammatory cytokine and chemokine expression of gastroduodenal lesions in Crohn's disease

Moriyama¹,

Matsumoto²,

Jo³

et al. 2005

Aliment Pharmacol Ther

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“…Some studies [1][2][3][4][5] demonstrated that H pylori can stimulate interleukin-8 (IL-8) production in gastric mucosal epithelia, which induces accumulation of neutrophilic granulocytes in mucosa. Chemotactic response initiates inflammatory damage to gastric mucosa, which plays a crucial role in the pathogenesis of H pylori.…”

Section: Introductionmentioning

confidence: 99%

Lactobacilli inhibit interleukin-8 production induced by Helicobacter pylori lipopolysaccharide-activated Toll-like receptor 4

Zhou¹,

Ma²,

Deng³

et al. 2008

WJG

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METHODS: S G C-7901 c el ls were t reat ed wi t h H pylori SS1-LPS in the presence or absence of pretreatment for 1 h with viable LBG or supernatant recovered from LBG culture MRS broth (LBG-S ). Cellular lysates were prepared for Western blot with anti-TLR4, anti-transforming growth factor β-activated kinase 1 (TAK1), anti-phospho-TAK1, anti-nuclear factor κB (NF-κB), anti-p38 mitogen-activated protein kinase (p38MAPK), and anti-phospho-p38MAPK antibodies. The amount of IL-8 in cell culture medium was measured by ELISA. R E S U LT S : H p y l o r i S S 1 -L P S u p -r e g u l a t e d t h eexpression of TLR4, stimulated the phosphorylation of TAK1, subsequently enhanced the activation of NF-κB and the phosphorylation of p38MAPK in a timedependent manner, leading to augmentation of IL-8 production in SGC-7901 cells. Viable LBG or LBG-S pretreatment attenuated the expression of TLR4, inhibited the phosphorylation of TAK1 and p38MAPK, prevented the activation of NF-κB, and consequently blocked IL-8 production.

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Lafutidine inhibits Helicobacter pylori‐induced interleukin‐8 production in human gastric epithelial cells

Abstract: These results suggest that lafutidine inhibits IL-8 release by inhibiting H. pylori adherence to gastric epithelial cells, indicating a novel mechanism by which lafutidine protects against the mucosal inflammation associated with H. pylori infection.

Cited by 8 publications

References 23 publications

Reduced lesions in chickens with Clostridium perfringens-induced necrotic enteritis by Lactobacillus fermentum 1.2029 ,

Reduced lesions in chickens with Clostridium perfringens-induced necrotic enteritis by Lactobacillus fermentum 1.2029 ,

Mucosal proinflammatory cytokine and chemokine expression of gastroduodenal lesions in Crohn's disease

Lactobacilli inhibit interleukin-8 production induced by Helicobacter pylori lipopolysaccharide-activated Toll-like receptor 4

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