Lactobacilli inhibit interleukin-8 production induced by Helicobacter pylori lipopolysaccharide-activated Toll-like receptor 4

Abstract

METHODS: S G C-7901 c el ls were t reat ed wi t h H pylori SS1-LPS in the presence or absence of pretreatment for 1 h with viable LBG or supernatant recovered from LBG culture MRS broth (LBG-S ). Cellular lysates were prepared for Western blot with anti-TLR4, anti-transforming growth factor β-activated kinase 1 (TAK1), anti-phospho-TAK1, anti-nuclear factor κB (NF-κB), anti-p38 mitogen-activated protein kinase (p38MAPK), and anti-phospho-p38MAPK antibodies. The amount of IL-8 in cell culture medium was measured by ELISA. R E S U LT S : H p y l o r i S S 1 -L P S u p -r e g u l a t e d t h eexpression of TLR4, stimulated the phosphorylation of TAK1, subsequently enhanced the activation of NF-κB and the phosphorylation of p38MAPK in a timedependent manner, leading to augmentation of IL-8 production in SGC-7901 cells. Viable LBG or LBG-S pretreatment attenuated the expression of TLR4, inhibited the phosphorylation of TAK1 and p38MAPK, prevented the activation of NF-κB, and consequently blocked IL-8 production.

Role for TAK1 in cigarette smoke-induced proinflammatory signaling and IL-8 release by human airway smooth muscle cells

“…TAK1 has been demonstrated to activate NF-B and MAP kinase pathways, which may lead to increased production of proinflammatory cytokines, including IL-6, IL-8, and TNF-␣ (5,37,41,53). Consistent with our findings, TAK1 has been shown to mediate IL-8 release from various cell types in response to the proinflammatory stimuli IL-1␤ (16), TNF-␣ (9,48), and LPS (9,53). A role for TAK1 in CS-induced cellular responses has not previously been reported.…”

Anti‐inflammatory Properties of Gastric‐derived Lactobacillus plantarum XB7 in the Context of Helicobacter pylori Infection

“…Accumulating evidence has demonstrated the beneficial effects of Lactobacillus spp. in the reduction of H. pylori ‐induced IL‐8 production in vitro and in vivo, but almost all are not originated from the stomach .…”

“…Viable L. gasseri OLL2716 suppressed H. pylori‐ induced IL‐8 production from MKN45 cells in vitro , and in vivo from biopsies of human gastric mucosa . Recently, L. bulgaricus has been reported to specifically inhibit H. pylori SS1‐LPS‐induced IL‐8 production by the inhibition of Toll‐like receptor (TLR) 4 pathway in SGC‐7901 human gastric adenocarcinoma cells .…”

Developmental biology of gut-probiotic interaction

“…47 Recent studies suggest that probiotics may modulate these aberrant TLR-4 responses and protect against excessive inflammatory signaling in immature epithelia. LPSmediated TLR-4 activation and IL-8 production is inhibited by Lactobacillus bulgaricus treatment in adult gastric cells 48 and regulation of intestinal development, such as fucosylation, are normalized once these germ-free mice are exposed to commensal strains. 73 These findings support the critical importance of commensal colonization in gut development and immunity and provide a rationale for promoting the growth of helpful commensal bacteria either through judicious use of antibiotics or the administration of prebiotic or probiotic agents in conditions, such as prematurity, where this essential process is disrupted.…”