Lactobacillus paracasei S16 Alleviates Lumbar Disc Herniation by Modulating Inflammation Response and Gut Microbiota

Abstract

Lumbar disc herniation (LDH) is a common cause for low back pain. In this study, we aimed to explore the effects of a specific Lactobacillus paracasei (L. paracasei), L. paracasei S16, on the symptoms of LDH using a mouse model of LDH. The results showed that L. paracasei S16 treatment improved the behavior, increased the cell proliferation, and decreased the apoptosis in LDH mice. Moreover, L. paracasei S16 treatment alleviated the aberrant inflammation response in the LDH mice, which is characterized by the decreased anti-inflammatory cytokines, increased pro-inflammatory cytokines, and decreased percentage of Th1 and Th2 cells and Th17/Treg ratio. 16S rRNA sequencing results showed that the LDH mice treated with L. paracasei S16 have higher relative abundance of Lachnospiraceae and Ruminococcaceae and lower abundance of Lactobacillaceae than mice in the LDH group. Additionally, the serum metabolites involved in the linoleic acid metabolism, alanine. aspartate, and glutamate, glycerophospholipid, and TCA cycle were significantly decreased and the metabolite involved in purine metabolism was significantly increased after the L. paracasei S16 treatment in the LDH mice. These results showed that administration of L. paracasei S16 can improve inflammation response, alter gut microbiota, and modulate serum metabolomics in a mouse model of LDH.

Gut Microbiome in Anesthesiology and Pain Medicine

“…Lumbar disc herniation mice receiving L. paracasei , but not sham, exhibited an attenuated behavioral pain response and decreased inflammatory response in the dorsal root ganglia. 112…”

Intestinal Microbiomics and Metabolomics Insights into the Hepatoprotective Effects of Lactobacillus paracasei CCFM1222 Against the Acute Liver Injury in Mice

“…For example, L. paracasei has been proved to suppress the in ammatory cytokines secretion (including TNF-α, IL-6, IFNγ), and elevate the antioxidant enzymes activities [including catalase (CAT) and superoxide dismutase (SOD)] [11]. L. paracasei S16 intervention also ameliorated the aberrant in ammation response and reduced the percentage of Th1 and Th2 cells and Th17/Treg ratio [12]. Furthermore, a previous study elaborated that Long-term intervention of L. paracasei remarkably elevated the proportion of bene cial bacteria and remarkably decreased the proportion of harmful bacteria [13].…”

Genetically supported causality between gut microbiota, gut metabolites and low back pain: a two-sample Mendelian randomization study

“…Rajasekaran et al (2020) found a higher abundance of Oxalobacter in India human degenerated intervertebral disc and Lactobacillus was found to be abundant in normal disc compared to disc herniation, which follows a similar trend to our results. In lumbar disc herniation mouse models, a relatively high abundance of Ruminococcaceae in gut is associated with improved behavior, increased cell proliferation and decreased apoptosis (Wang et al, 2021). Therefore, gut microbiota may affect LBP mediating dysbiosis of microbiota in intervertebral disc.…”