Lactobacillus crispatus and its enolase and glutamine synthetase influence interactions between Neisseria gonorrhoeae and human epithelial cells

Płaczkiewicz, Jagoda; Chmiel, Paulina; Malinowska, Ewelina; Bącal, Paweł; Kwiatek, Agnieszka

doi:10.1007/s12275-020-9505-9

Cited by 9 publications

(9 citation statements)

References 31 publications

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“…According to data previously reported for the L. plantarum GAPDH [74], Kaiulainen et al [73] suggested that incorporation into L. crispatus cell wall of these proteins is affected by changes in cellular permeability. More recently it was also demonstrated that L. crispatus enolase and GS have a role in protecting epithelium against Neisseria gonorrhoeae infections [75]. L. plantarum 299V GAPDH and enolase were also found to mediate adhesion to fibronectin whereas only GAPDH was able to weakly bind to mucin [76].…”

Section: Role Of Moonlighting Proteins In Adhesion Of Lactobacillimentioning

confidence: 97%

Lactobacillus Cell Surface Proteins Involved in Interaction with Mucus and Extracellular Matrix Components

2020

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The gut microbiota is a complex microbial ecosystem where bacteria, through mutual interactions, cooperate in maintaining of wellbeing and health. Lactobacilli are among the most important constituents of human and animal intestinal microbiota and include many probiotic strains. Their presence ensures protection from invasion of pathogens, as well as stimulation of the immune system and protection of the intestinal flora, often exerted through the ability to interact with mucus and extracellular matrix components. The main factors responsible for mediating adhesion of pathogens and commensals to the gut are cell surface proteins that recognize host targets, as mucus layer and extracellular matrix proteins. In the last years, several adhesins have been reported to be involved in lactobacilli–host interaction often miming the same mechanism used by pathogens.

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Section: Role Of Moonlighting Proteins In Adhesion Of Lactobacillimentioning

confidence: 97%

Lactobacillus Cell Surface Proteins Involved in Interaction with Mucus and Extracellular Matrix Components

2020

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“…These adhesins are involved not only in Lactobacillus adhesion but also in preventing pathogens’ interactions to host receptors by competitive inhibition [ 31 , 35 ]. In particular, EVs from L. crispatus BC5 and L. gasseri BC12 delivered enolase-1 that resulted to reduce Neisseria ghonorreae attachment to host cells [ 8 , 36 , 37 ]. Moreover, L. gasseri BC12-EVs transported two more adhesins (i.e., enolase-2 and elongation factor-TU) that inhibited E. coli adhesion to host mucosa [ 8 , 35 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

Lactobacilli extracellular vesicles: potential postbiotics to support the vaginal microbiota homeostasis

et al. 2022

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Background Lactobacillus species dominate the vaginal microflora performing a first-line defense against vaginal infections. Extracellular vesicles (EVs) released by lactobacilli are considered mediators of their beneficial effects affecting cellular communication, homeostasis, microbial balance, and host immune system pathways. Up to now, very little is known about the role played by Lactobacillus EVs in the vaginal microenvironment, and mechanisms of action remain poorly understood. Results Here, we hypothesized that EVs can mediate lactobacilli beneficial effects to the host by modulating the vaginal microbiota colonization. We recovered and characterized EVs produced by two vaginal strains, namely Lactobacillus crispatus BC5 and Lactobacillus gasseri BC12. EVs were isolated by ultracentrifugation and physically characterized by Nanoparticle Tracking Analysis (NTA) and Dynamic Light Scattering (DLS). EVs protein and nucleic acids (DNA and RNA) content was also evaluated. We explored the role of EVs on bacterial adhesion and colonization, using a cervical cell line (HeLa) as an in vitro model. Specifically, we evaluated the effect of EVs on the adhesion of both vaginal beneficial lactobacilli and opportunistic pathogens (i.e., Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae, and Enterococcus faecalis). We demonstrated that EVs from L. crispatus BC5 and L. gasseri BC12 significantly enhanced the cellular adhesion of all tested lactobacilli, reaching the maximum stimulation effect on strains belonging to L. crispatus species (335% and 269% of average adhesion, respectively). At the same time, EVs reduced the adhesion of all tested pathogens, being EVs from L. gasseri BC12 the most efficient. Conclusions Our observations suggest for the first time that EVs released by symbiotic Lactobacillus strains favor healthy vaginal homeostasis by supporting the colonization of beneficial species and preventing pathogens attachment. This study reinforces the concept of EVs as valid postbiotics and opens the perspective of developing postbiotics from vaginal strains to maintain microbiota homeostasis and promote women’s health.

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“…This may be due to the fact that L. iners is incapable of downregulating histone deacetylase 4 and does not sufficiently reduce cell proliferation to protect against C. trachomatis infection ( Petrova et al., 2017 ; Edwards et al., 2019 ). Conversely, other species of the genus Lactobacillus such as L. jensenii , L. crispatus , and L. gasseri are capable of producing D-lactic acid, bacteriocins, and other antimicrobial compounds to protect against sexually transmitted pathogens, including C. trachomatis , Neisseria gonorrhoeae , and HPV ( Brotman et al., 2014 ; Placzkiewicz et al., 2020 ). In particular, L. crispatus was reported to suppress the adhesion and infectivity of C. trachomatis in human epithelial cells ( Nardini et al., 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Alterations of Vaginal Microbiota in Women With Infertility and Chlamydia trachomatis Infection

Chen

Wang

Zhao

et al. 2021

Front. Cell. Infect. Microbiol.

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Chlamydia trachomatis (C. trachomatis) is the most common etiological agent of bacterial sexually transmitted infections (STIs) worldwide and causes serious health sequelae such as cervicitis, pelvic inflammatory disease, and even infertility if ascending from the lower to the upper female genital tract. Previous studies have revealed the pivotal role of vaginal microbiota in susceptibility to STIs. However, alterations in the vaginal microbiota in women who are infertile and infected with C. trachomatis remain unknown. This study used metagenomic analysis of sequenced 16S rRNA gene amplicons to examine the vaginal microbial profiles of women with tubal infertility who were C. trachomatis-negative and those who were C. trachomatis-positive pre- and post-antibiotic treatment. Women who were C. trachomatis-negative and deemed healthy were recruited as references of eubiosis and dysbiosis. Women with tubal infertility and C. trachomatis infection presented a unique Lactobacillus iners-dominated vaginal microbiota rather than one dominated by Lactobacillus crispatus and displayed a decrease in Lactobacillus, Bifidobacterium, Enterobacter, Atopobium, and Streptococcus, accompanied by decreased levels of cytokines such as interferon (IFN)-γ and interleukin (IL)-10. This altered vaginal microbiota could be restored with varying degrees after standard treatment for C. trachomatis. This shift could be a predictive vaginal microbiota signature for C. trachomatis infection among females with tubal infertility, while no significant differences in phylum, class, and operational taxonomic unit (OTU) levels were observed between women with tubal infertility who were C. trachomatis-negative and healthy controls. This is the first study to provide data on the association of vaginal microbiota with C. trachomatis infection among women with tubal infertility and highlights unprecedented potential opportunities to predict C. trachomatis infection.

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Lactobacillus crispatus and its enolase and glutamine synthetase influence interactions between Neisseria gonorrhoeae and human epithelial cells

Cited by 9 publications

References 31 publications

Lactobacillus Cell Surface Proteins Involved in Interaction with Mucus and Extracellular Matrix Components

Lactobacillus Cell Surface Proteins Involved in Interaction with Mucus and Extracellular Matrix Components

Lactobacilli extracellular vesicles: potential postbiotics to support the vaginal microbiota homeostasis

Alterations of Vaginal Microbiota in Women With Infertility and Chlamydia trachomatis Infection

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