Lactobacillus acidophilus NS1 Reduces Phosphoenolpyruvate Carboxylase Expression by Regulating HNF4α Transcriptional Activity

Yang, Garam; Kim, Eungseok

doi:10.5851/kosfa.2017.37.4.529

Cited by 11 publications

(7 citation statements)

References 20 publications

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“…We noticed an insignificant but definite reduction in the hepatic expressions of PEPCK and PGC1α genes in the HFD+F-fed mice in response to treatment with XEN or fPR. An earlier study concluded that suppression of hepatic PEPCK expression may be a preventive or therapeutic strategy for metabolic diseases [67]. We also observed a significant reduction in the hepatic expression of PPARγ gene in the HFD+F group upon exposure to XEN and fPR, but not to PR.…”

Section: Effects Of Pr and Fpr On Adipocyte Size And Pathways Relatedsupporting

confidence: 70%

Lactate-Fortified Puerariae Radix Fermented by Bifidobacterium breve Improved Diet-Induced Metabolic Dysregulation via Alteration of Gut Microbial Communities

Choi

Bose²,

Shin

et al. 2020

Nutrients

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Background: Puerariae Radix (PR), the dried root of Pueraria lobata, is reported to possess therapeutic efficacies against various diseases including obesity, diabetes, and hypertension. Fermentation-driven bioactivation of herbal medicines can result in improved therapeutic potencies and efficacies. Methods: C57BL/6J mice were fed a high-fat diet and fructose in water with PR (400 mg/kg) or PR fermented by Bifidobacterium breve (400 mg/kg) for 10 weeks. Histological staining, qPCR, Western blot, and 16s rRNA sequencing were used to determine the protective effects of PR and fermented PR (fPR) against metabolic dysfunction. Results: Treatment with both PR and fPR for 10 weeks resulted in a reduction in body weight gain with a more significant reduction in the latter group. Lactate, important for energy metabolism and homeostasis, was increased during fermentation. Both PR and fPR caused significant down-regulation of the intestinal expression of the MCP-1, IL-6, and TNF-α genes. However, for the IL-6 and TNF-α gene expressions, the inhibitory effect of fPR was more pronounced (p < 0.01) than that of PR (p < 0.05). Oral glucose tolerance test results showed that both PR and fPR treatments improved glucose homeostasis. In addition, there was a significant reduction in the expression of hepatic gene PPARγ, a key regulator of lipid and glucose metabolism, following fPR but not PR treatment. Activation of hepatic AMPK phosphorylation was significantly enhanced by both PR and fPR treatment. In addition, both PR and fPR reduced adipocyte size in highly significant manners (p < 0.001). Treatment by fPR but not PR significantly reduced the expression of PPARγ and low-density lipoproteins in adipose tissue. Conclusion: Treatment with fPR appears to be more potent than that of PR in improving the pathways related to glucose and lipid metabolism in high-fat diet (HFD)+fructose-fed animals. The results revealed that the process of fermentation of PR enhanced lactate and facilitated the enrichment of certain microbial communities that contribute to anti-obesity and anti-inflammatory activities.

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Section: Effects Of Pr and Fpr On Adipocyte Size And Pathways Relatedsupporting

confidence: 70%

Lactate-Fortified Puerariae Radix Fermented by Bifidobacterium breve Improved Diet-Induced Metabolic Dysregulation via Alteration of Gut Microbial Communities

Choi

Bose²,

Shin

et al. 2020

Nutrients

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“…Park et al [ 159 ] decreased gene expression of the enzyme PEPCK, which is critical for hepatic gluconeogenesis, by supplementing high-fat diet-fed mice with Lactobacillus acidophilus NS1 (NS1). The reduced expression of the enzyme was also confirmed in vitro by demonstrating that NS1 regulates reduction by activating transcription of hepatocyte nuclear factor 4 alpha (HNF4α), a core receptor and a key element in hepatic differentiation and cell proliferation [ 160 ].…”

Section: Effect Of Probiotics On Glucose Metabolism and Homeostasismentioning

confidence: 99%

Probiotic Mechanisms Affecting Glucose Homeostasis: A Scoping Review

Pintarič

Langerholc

2022

Life

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The maintenance of a healthy status depends on the coexistence between the host organism and the microbiota. Early studies have already focused on the nutritional properties of probiotics, which may also contribute to the structural changes in the gut microbiota, thereby affecting host metabolism and homeostasis. Maintaining homeostasis in the body is therefore crucial and is reflected at all levels, including that of glucose, a simple sugar molecule that is an essential fuel for normal cellular function. Despite numerous clinical studies that have shown the effect of various probiotics on glucose and its homeostasis, knowledge about the exact function of their mechanism is still scarce. The aim of our review was to select in vivo and in vitro studies in English published in the last eleven years dealing with the effects of probiotics on glucose metabolism and its homeostasis. In this context, diverse probiotic effects at different organ levels were highlighted, summarizing their potential mechanisms to influence glucose metabolism and its homeostasis. Variations in results due to different methodological approaches were discussed, as well as limitations, especially in in vivo studies. Further studies on the interactions between probiotics, host microorganisms and their immunity are needed.

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“…Liver activation of FXR by probiotics contributed to inhibit hepatic gluconeogenesis-related enzymes . Consistently, Lactobacillus acidophilus NS1 improved blood glucose levels and insulin resistance in HFD-fed mice via suppressing the key hepatic gluconeogenesis enzyme [phosphoenolpyruvate carboxykinase (PEPCK)] . Another probiotic treatment study showed that Lactobacillus plantarum MTCC5690 and Lactobacillus fermentum MTCC5689 could increase expression of PEPCK, G6Pc, and FOXO1 and enhance hepatic gluconeogenesis in HFD-fed diabetic mice …”

Section: Mechanism Insights In Probiotics Contributing To the Regulat...mentioning

confidence: 87%

“…65 Consistently, Lactobacillus acidophilus NS1 improved blood glucose levels and insulin resistance in HFD-fed mice via suppressing the key hepatic gluconeogenesis enzyme [phosphoenolpyruvate carboxykinase (PEPCK)]. 66 Another probiotic treatment study showed that Lactobacillus plantarum MTCC5690 and Lactobacillus fermentum MTCC5689 could increase expression of PEPCK, G6Pc, and FOXO1 and enhance hepatic gluconeogenesis in HFD-fed diabetic mice. 67 Except FXR, another important BA receptor is TGR5, whose activation in liver Kupffer cells can also exert anti-inflammatory activities and improve HFD-induced glucose intolerance.…”

Section: ■ Introductionmentioning

confidence: 97%

Different Types and Functional Effects of Probiotics on Human Health through Regulating Glucose Homeostasis

Zhang

Sun

Qian

et al. 2021

J. Agric. Food Chem.

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With the increasing improvement of people's living standards, hyperglycemia has become one of the most frequent diseases in the world. The current drug therapy may have some negative effects and even cause some complications. As one of the most popular functional ingredients, probiotic bacteria have been proven to play important roles in balancing the glucose homeostasis level in animal and human clinic trials. In this perspective, we sorted three types of probiotics, discussed probiotic safety evaluation, and listed the known probiotic functional foods that assist to control glucose homeostasis. Then, the further summarization of the mechanisms on how probiotic bacteria could regulate glucose homeostasis and the developing trend of probiotic functional foods were discussed.

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Lactobacillus acidophilus NS1 Reduces Phosphoenolpyruvate Carboxylase Expression by Regulating HNF4α Transcriptional Activity

Cited by 11 publications

References 20 publications

Lactate-Fortified Puerariae Radix Fermented by Bifidobacterium breve Improved Diet-Induced Metabolic Dysregulation via Alteration of Gut Microbial Communities

Lactate-Fortified Puerariae Radix Fermented by Bifidobacterium breve Improved Diet-Induced Metabolic Dysregulation via Alteration of Gut Microbial Communities

Probiotic Mechanisms Affecting Glucose Homeostasis: A Scoping Review

Different Types and Functional Effects of Probiotics on Human Health through Regulating Glucose Homeostasis

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