Probiotics are known to contribute to the anti-oxidation, immunoregulation, and aging delay. Here, we investigated the extension of lifespan by fermented pickles-origin Pediococcus acidilactici (PA) in Caenorhabditis elegans (C. elegans), and found that PA promoted a significantly extended longevity of wild-type C. elegans. The further results revealed that PA regulated the longevity via promoting the insulin/IGF-1 signaling, JNK/MAPK signaling but not TOR signaling in C. elegans, and that PA reduced the reactive oxygen species (ROS) levels and modulated expression of genes involved in fatty acids uptake and lipolysis, thus reducing the fat accumulation in C. elegans. Moreover, this study identified the nrfl-1 as the key regulator of the PA-mediated longevity, and the nrfl-1/daf-18 signaling might be activated. Further, we highlighted the roles of one chloride ion exchanger gene sulp-6 in the survival of C. elegans and other two chloride ion channel genes clh-1 and clh-4 in the prolonged lifespan by PA-feeding through the modulating expression of genes involved in inflammation. Therefore, these findings reveal the detailed and novel molecular mechanisms on the longevity of C. elegans promoted by PA.
With the increasing improvement of people's living standards, hyperglycemia has become one of the most frequent diseases in the world. The current drug therapy may have some negative effects and even cause some complications. As one of the most popular functional ingredients, probiotic bacteria have been proven to play important roles in balancing the glucose homeostasis level in animal and human clinic trials. In this perspective, we sorted three types of probiotics, discussed probiotic safety evaluation, and listed the known probiotic functional foods that assist to control glucose homeostasis. Then, the further summarization of the mechanisms on how probiotic bacteria could regulate glucose homeostasis and the developing trend of probiotic functional foods were discussed.
Amino acid intake plays a crucial role in the Warburg effect of cancer. The gut microbes could regulate intestinal amino acid metabolism. However, it is still unknown whether a probiotic therapy can protect the host from intestinal tumor invasion by reducing amino acid intake. With in vitro methods, three acid tolerant strains from fermented pickles were screened out. Using AOM/DSS induced colon cancer models, we evaluated the therapeutic effects of Lactobacillus brevis CLB3, Lactobacillus plantarum XLP and Lactobacillus johnsonii CM on model mice. Their functional mechanism were further explained through anatomy section, qRT-PCR, Western blot, and immunohistochemical staining analyses as well as database mining and gut culturomics. The L. brevis CLB3 treatment significantly improved clinical signs and symptoms of colon cancer, alleviated colon damage, and inhibited colon carcinogenesis in mice. In addition, this treatment significantly increased gut cultivable Lactobacillus abundance, inhibited the expression and translation levels of the tumor metabolism-related solute carrier (SLC) amino acid transporter including SLC7A5 and SLC7A11, lowered circulating IL-6 and IL-17A levels, and improved the accumulation of tumor-infiltrating lymphocytes and cancer proliferation factors. These findings suggest that L. brevis CLB3 can reduce amino acid transport, inhibit mTOR signaling and enhance intestinal anti-tumor immune responses, which provides a potential targeting amino acid transporters strategy for preventing colorectal cancer.
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