Lactic acid bacteria with cholesterol-lowering properties for dairy applications: In vitro and in situ activity

Albano, Clara; Morandi, Stefano; Silvetti, Tiziana; Casiraghi, Maria Cristina; Manini, Federica; Brasca, Milena

doi:10.3168/jds.2018-15096

Cited by 71 publications

(63 citation statements)

References 64 publications

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“…Cholesterol lowering capability is one of the essential characteristics of lactic acid bacteria with probiotic potential. 41,70 It prompted us to investigate the efficiency of CFM of A29 on fat depositions in 3T3-L1 adipocytes. Furthermore, Bodyweight, adipose tissue mass reduction, and clinical parameter alterations in the blood by live A29 supplement to HFD-fed mice were evaluated.…”

Section: Discussionmentioning

confidence: 99%

Positive metabolic effects of selected probiotic bacteria on diet‐induced obesity in mice are associated with improvement of dysbiotic gut microbiota

et al. 2020

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Given the rising evidence that gut malfunction including changes in the gut microbiota composition, plays a major role in the development of obesity and associated metabolic diseases, the exploring of novel probiotic bacteria with potential health benefits has attracted great attention. Recently Lactobacillus spp., exert potent anti‐obesity effects by regulating key transcriptional and translational factors in adipose tissues. However, the molecular mechanism behind the anti‐obesity effect of probiotics is not yet fully understood. Therefore, we investigated the effect of Lactobacillus plantarum A29 on the expression of adipogenic and lipogenic genes in 3T3‐L1 adipocytes and high‐fat diet (HFD)‐fed mice. We observed that the treatment of 3T3‐L1 adipocytes with the cell‐free metabolites of L plantarum inhibited their differentiation and fat depositions via downregulating the key adipogenic transcriptional factors (PPAR‐γ, C/EBP‐α, and C/EBP‐β) and their downstream targets (FAS, aP2, ACC, and SREBP‐1). Interestingly, supplementation with L plantarum reduced the fat mass and serum lipid profile concurrently with downregulation of lipogenic gene expression in the adipocytes, resulting in reductions in the bodyweight of HFD‐fed obese mice. L plantarum treatment attenuated the development of obesity in HFD‐fed mice via the activation of p38MAPK, p44/42, and AMPK‐α by increasing their phosphorylation. Further analysis revealed that A29 modulated gut‐associated microbiota composition. Thus, A 29 potential probiotic strain may alleviate the obesity development and its associated metabolic disorders via inhibiting PPARγ through activating the p38MAPK and p44/42 signaling pathways.

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Section: Discussionmentioning

confidence: 99%

Positive metabolic effects of selected probiotic bacteria on diet‐induced obesity in mice are associated with improvement of dysbiotic gut microbiota

et al. 2020

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“…Lee et al (2011) isolated 12 Lactobacillus strains from kimchi and showed the ability to reduce cholesterol level by more than 40% in the in vitro test. Albano et al (2018) reported that Lactobacillus casei VC199, Lactobacillus paracasei ssp. paracasei SE160 and VC213, Lact.…”

Section: Introductionmentioning

confidence: 99%

Cholesterol homeostasis associated with probiotic supplementation in vivo

Lim

Loke

et al. 2020

J. Appl. Microbiol.

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Aims: To determine the mechanism underlying the serum cholesterol reduction effect by probiotics isolated from local fermented tapioca (Tapai). Methods and Results: Lactic acid bacteria strains were isolated and examined for acid tolerance, bile salt resistance and hypocholesterolemic properties. Among the isolates, Lactobacillus plantarum TAR4 showed the highest cholesterol reduction ability (48Á01%). The focus in the in vivo trial was to elucidate the cholesterol balance from findings pertaining to serum cholesterol reduction in rat model fed with high fat diet via oral administration. Rats fed with high-cholesterol diet supplemented with Lact. plantarum TAR4 showed significant reduction in serum total cholesterol (29Á55%), serum triglyceride (45Á31%) and liver triglyceride (23Á44%) as compared to high-cholesterol diet (HCD) group. There was a significant increment in faecal triglyceride (45Á83%) and faecal total bile acid (384Á95%) as compared to HCD group. Conclusions: The findings showed that probiotic Lact. plantarum TAR4 supplementation reduced the absorption of bile acids for enterohepatic recycling and increased the catabolism of cholesterol to bile acids and not by suppressing the rate of cholesterol synthesis. Significance and Impact of Study: Probiotic supplements could provide a new nonpharmacological alternative to reduce cardiovascular risk factors. Key Points • Lactobacillus plantarum TAR4 exhibited an excellent tolerance to acid and bile salts. • Lactobacillus plantarum TAR4 was able to assimilate cholesterol in vitro. • Lactobacillus plantarum TAR4 had the ability to regulate cholesterol metabolism levels in rats. • Hypocholesterolemic effect related to cholesterol binding and deconjugation of bile acid by Lactobacillus plantarum TAR4.

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“…Though studies have reported the BSH activity, hypo-cholesterolemic effect, and probiotic properties of Enterococcus strains isolated from animal and human gut (Hlivak et al, 2005; Hyrslova et al, 2016; Zhang et al, 2017) and fermented food products (Guo et al, 2016; Albano et al, 2018; Nami et al, 2019), the BSH activity and cholesterol-lowering ability of Enterococcus isolated from environmental niches, like aquatic and terrestrial plants, soil, and water, have not been reported. Bacteria isolated from environments in which bile salts are absent usually do not demonstrate BSH activity (Elkins et al, 2001; Moser and Savage, 2001; Ahn et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Bile Salt Hydrolases, Cholesterol-Lowering Capabilities, and Probiotic Potential of Enterococcus faecium Isolated From Rhizosphere

et al. 2019

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Bile salt hydrolase (BSH) activity, hypo-cholesterolemic effect, and probiotic properties have been reported for Enterococcus strains isolated from animal and human gut and fermented foods but not for strains isolated from environmental niches, like aquatic and terrestrial plants, soil, and water. The present study is the first report on isolation of Enterococcus faecium from rhizospheric soils that harbor the bsh gene, remove cholesterol in vitro , and possess essential and desirable probiotic attributes. Fifteen samples were collected from different sites located in northern, southern, and central regions of India, of which five yielded pure colonies that were named LR2, LR3, ER5, LR13, and VB1. These were identified by 16S rRNA gene sequencing as E. faecium and evaluated for BSH activity, cholesterol-lowering potential in vitro , and probiotic properties. Our results indicated that all the strains were capable of surviving the harsh conditions of the gastrointestinal tract and did not harbor any of the virulence genes. Though all strains showed the presence of bsh and potential for cholesterol removal, E. faecium strain LR13 showed a remarkable cholesterol removal capability and vancomycin susceptibility and possessed most of the desirable and essential attributes of a probiotic. Hence, it seems to be a fairly promising probiotic candidate that needs to be further evaluated in in vivo studies, especially for its hypo-cholesterolemic potential.

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Lactic acid bacteria with cholesterol-lowering properties for dairy applications: In vitro and in situ activity

Cited by 71 publications

References 64 publications

Positive metabolic effects of selected probiotic bacteria on diet‐induced obesity in mice are associated with improvement of dysbiotic gut microbiota

Positive metabolic effects of selected probiotic bacteria on diet‐induced obesity in mice are associated with improvement of dysbiotic gut microbiota

Cholesterol homeostasis associated with probiotic supplementation in vivo

Evaluation of Bile Salt Hydrolases, Cholesterol-Lowering Capabilities, and Probiotic Potential of Enterococcus faecium Isolated From Rhizosphere

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