Lactate, glutamate and glutamine biosensors based on rhodinised carbon electrodes

White, Stephen F.; Turner, Anthony P. F.; Bilitewski, Ursula; Schmid, Rolf D.; Bradley, J.

doi:10.1016/0003-2670(94)80229-7

Cited by 56 publications

(31 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…HRP activity was measured using hexacyanoferrate (II/III) mediator at bare carbon electrodes (-20mV vs. Ag/AgCl) using hydrogen peroxide as substrate. GOX activity was measured at a rhodinised carbon electrode (+350 mV vs. Ag/AgCl), a suitable surface for the mediator-free oxidation of the enzymatic reaction product, hydrogen peroxide [46][47][48].…”

Section: Indirect Immunosensing In the Organic-phasementioning

confidence: 99%

Organic phase immunosensors

Setford¹,

Kröger²,

Turner³

1999

Analusis

View full text Add to dashboard Cite

Many analytical methods involving non-polar analytes require solvent extraction prior to measurement. The analysis procedure is greatly simplified if the method is able to function effectively in the more non-polar solvent extract. This consideration, coupled with the increasing need for simple, specific and rapid diagnostic and screening tools, has focused interest in the development of organic-phase immunosensors.

show abstract

Section: Indirect Immunosensing In the Organic-phasementioning

confidence: 99%

Organic phase immunosensors

Setford¹,

Kröger²,

Turner³

1999

Analusis

View full text Add to dashboard Cite

show abstract

“…The use of modified electrodes for the amperometric detection of hydrogen peroxide is a suitable way of constructing oxidase based enzyme sensors [11,12], however the construction of devices with highly effective electrical communication among the redox centres of enzymes and electrodes is still an important target. Oxidase based enzyme sensors for determination of glucose and lactate are two of the most popular and well-known applications of biosensors.…”

Section: Introductionmentioning

confidence: 99%

Multioperational Oxidase Biosensors Based on Carbosilane Dendrimers with Interacting Ferrocenes

et al. 2011

View full text Add to dashboard Cite

The bioelectrocatalytical properties and kinetic characteristics of new oxidase biosensors based on two different carbosilane dendrimers are described. The best glucose biosensor developed displayed, in an ascorbate interference free work potential interval, a strictly linear range from 0 to 4.0 mM, a detection limit of 40,6 mM and a response time less than 3 s. The lactate biosensor displayed a linear range from 0 to 0.8 mM, a detection limit of 0.73 mM and a response time less than 2 s. The apparent Michaelis-Menten constants were calculated to be 4.39 mM and 2.08 mM respectively, according to Lineweaver-Burk equation.

show abstract

“…A variety of chemistries have been explored for efficient detection of H 2 O 2 generated by GluOx, including direct oxidation on metals 12, [24][25][26][27][28][29] (usually coated with a permselective polymer to block interference), metallicized carbon 30,31 or conducting polymercoated Pt, 32 and catalytic oxidation using horseradish peroxidase coupled to a redox polymer. 6,15,20,33,34 Biosensors in general, and especially those designed for tissue implantation, [35][36][37][38][39] must fulfill the following minimum criteria for reliable monitoring of the target analyte: appropriate size and biocompatibility properties, good sensitivity to the enzyme substrate, effective rejection of electroactive interference, and low sensitivity to changes in pO 2 over the range of substrate and oxygen concentrations relevant to the intended application.…”

mentioning

confidence: 99%

Control of the Oxygen Dependence of an Implantable Polymer/Enzyme Composite Biosensor for Glutamate

et al. 2006

View full text Add to dashboard Cite

Biosensors for glutamate (Glu) were fabricated from Teflon-coated Pt wire (cylinders and disks), modified with the enzyme glutamate oxidase (GluOx) and electrosynthesized polymer PPD, poly(o-phenylenediamine). The polymer/enzyme layer was deposited in two configurations: enzyme before polymer (GluOx/PPD) and enzyme after polymer (PPD/GluOx). These four biosensor designs were characterized in terms of response time, limit of detection, Michaelis-Menten parameters for Glu (J max and K M (Glu)), sensitivity to Glu in the linear response region, and dependence on oxygen concentration, K M (O 2 ). Analysis showed that the two polymer/enzyme configurations behaved similarly on both cylinders and disks. Although the two geometries showed different behaviors, these differences could be explained in terms of higher enzyme loading density on the disks; in many analyses, the four designs behaved like a single population with a range of GluOx loading. Enzyme loading was the key to controlling the K M (O 2 ) values of these first generation biosensors. The counterintuitive, and beneficial, behavior that biosensors with higher GluOx loading displayed a lower oxygen dependence was explained in terms of the effects of enzyme loading on the affinity of GluOx for its anionic substrate. Some differences between the properties of surface immobilized GluOx and glucose oxidase are highlighted.L-Glutamate (Glu) is the most widespread excitatory neurotransmitter in the mammalian central nervous system, 1 plays a major role in a broad range of brain functions, and has been implicated in a number of neurological disorders. 2 Indeed, the development of devices for Glu detection has become an important research area due to the value of monitoring this key amino acid in a number of complex matrixes, including food processing, 3,4 cell cultures, 5-7 tissue slices ex vivo, 8,9 and intact brain in vivo. [10][11][12][13][14][15] A number of designs of biosensors for Glu monitoring have been reported and, although Glu receptors 16 Brain Res. Bull. 2002, 58, 401-404. (10) Burmeister, J. J.; Gerhardt, G. A. Trends Anal. Chem. 2003, 22, 498-502. (11)

show abstract

Lactate, glutamate and glutamine biosensors based on rhodinised carbon electrodes

Cited by 56 publications

References 11 publications

Organic phase immunosensors

Organic phase immunosensors

Multioperational Oxidase Biosensors Based on Carbosilane Dendrimers with Interacting Ferrocenes

Control of the Oxygen Dependence of an Implantable Polymer/Enzyme Composite Biosensor for Glutamate

Contact Info

Product

Resources

About