Lactate dehydrogenase isoenzyme patterns in bronchogenic carcinoma

Langvad, E.

doi:10.1016/0014-2964(68)90074-1

Cited by 39 publications

(18 citation statements)

References 25 publications

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“…With this technique, we have found in polyps a significant increase of G 6 PD, pyruvate kinase, and total lactate dehydrogenase and lactate dehydrogenase M-monomers compared with that in normal mucosa. Similar changes have also been shown in the cancer group, the latter being in accordance with previous studies.912[4][5][6][7][8][9][10][11][12][13][14][15][16] Thus, examination ofenzyme activities does not seem to separate tubular adenomas from cancer.…”

supporting

confidence: 89%

Enzymatic characteristics of tubular adenomas and carcinomas of the large intestine.

Vatn¹,

Tjora²,

Arva³

et al. 1982

Gut

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SUMMARY Enzyme activities in homogenates of the large intestine were measured in biopsies from 11 tubular adenomas and six carcinomas and from the corresponding normal mucosa of each individual. A similar and significant increase was found in the total activities of glucose-6-phosphate dehydrogenase (G 6 PD) Received for publication 14 August 1981 As far as we know enzyme activities have not been measured in homogenates from tubular adenomas of the colon. The present study was carried out in order to see whether the above-mentioned difference in prognosis of tubular adenomas might be apparent from a shift in their enzyme patterns. MethodsPolyps were removed by sling resection, and biopsies from carcinomas and, for comparison, from normal mucosa were taken during routine colonoscopy. Biopsies from the normal mucosa were always taken more than 5 cm from the tumour. The specimens were quenched. When kept on ice, the polyps were cut, and two pieces from the parietal part of the polyp and samples from the other materials were stored within two hours at -70°C.

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supporting

confidence: 89%

Enzymatic characteristics of tubular adenomas and carcinomas of the large intestine.

Vatn¹,

Tjora²,

Arva³

et al. 1982

Gut

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“…The authors deemed the altered LDHA to LDHB ratio as a possible biochemical indicator of an early process of malignant conversion [67]. These conclusions were supported by the data of other investigators who showed high LDHA to LDHB ratios in uninvolved tissues from resection edges that were related to local recurrence possibly due to deep infiltration [58,59,[68][69][70][71]. It is known that LDH-5 overproduction is related exclusively to cancer cells but not to stromal fibroblasts, which have low LDHA expression [72].…”

Section: Clinical Significance Of Ldh Isoenzymesmentioning

confidence: 55%

“…The existence of subtle changes in morphologically normal tissues surrounding the malignant solid mass that predisposes to tumor formation has been already described and termed the cancer field effect (CFE) [73]. Thus, the estimation of the LDH isoenzyme ratio in tissue samples taken from adjacent to malignant tissue areas and from resection margins is proposed as a valuable prognostic indicator in surgical practice [69,74].…”

Section: Clinical Significance Of Ldh Isoenzymesmentioning

confidence: 99%

Lactate dehydrogenase 5: An old friend and a new hope in the war on cancer

2015

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“…Thus, in many human organs like the prostate, kidney, colon, mammary gland, lung and brain, the LDH isoenzyme M/H ratio increases in malignant tumours arising in these sites (Elhilani et al, 1967;Matsuda et al, 1980;Carda-Abella et al, 1982;Balinsky et al, 1983Balinsky et al, , 1984Tanaka et al, 1984;Fujii et al, 1984). Furthermore, Langvad (1968) reported that the M/H ratio increase of LDH precedes the histological changes which indicated malignancy in the bronchial mucous epithelium. The M/H increase of LDH isoenzymes has also been confirmed in extracts of human neoplastic bladder tissues (Bredin et al, 1975).…”

Section: Discussionmentioning

confidence: 99%

The diagnostic significance of lactate dehydrogenase isoenzymes in urinary cytology

Nishikawa

Tanaka²,

Takeuchi³

et al. 1991

Br J Cancer

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Summary Lactate dehydrogenase (LDH) isoenzyme distribution was examined in 106 urine samples being tested cytologically for evidence of bladder cancer; the samples were selected to have <20 leucocytes and erythrocytes per high power field and the LDH pattern determined by electrophoresis. The Papanicolaou stained-smears showed 68 negative, 17 suspicious and 21 positive. The LDH M-fraction of the urinary supernatant in cytologically positive cases was significantly greater than in negative cases, although the latter included a few false negative samples. Some of the false negatives gave positive results for the LDH M-fraction; these results suggest that the determination of LDH isoenzymes in the urine is useful in diagnosing urinary tract cancers, including early stage, and for follow-up of patients with bladder cancers after surgical resection.Because of such advantages as the ease of sample collection, the ability to test frequently and the absence of risk to patients, urine cytology has been regarded as one of the most useful diagnostic procedures for the screening of urinary tract neoplasms as well as for their post-operative follow-up (Fujii et al., 1982 (Fujii et al., 1982).The presence of lactate dehydrogenase (LDH) activity in urine was initially reported by Rosalki and Wilkinson (1959). Although Wacker and Dorfman (1962) noted elevated levels of LDH activity in the urine of patients with carcinoma of the urinary tract, the elevation of total urinary LDH activity is not now assumed to be cancer specific (Posey & Morgan, 1977). Increased urinary LDH may reflect contamination of urine with cells such as polymorphonuclear leucocytes, or proteinuria rather than the presence of neoplasia (Mirabile et al., 1966;Malik et al., 1983). It has also been suggested that urinary LDH isoenzyme patterns vary according to the site of inflammation in the urinary tract (Devaskar & Montogomery, 1978). However, unless urine is heavily contaminated with inflammatory cells, the M-fraction of urinary LDH may increase with the rising stage and the grade of the bladder tumour (Mortomiya et al., 1975(Mortomiya et al., , 1979. Therefore, it has been emphasised that the combination of urinary cytology and the determination of urinary LDH isoenzymes may be of value in the diagnosis of bladder tumours (Motomiya et al., 1975(Motomiya et al., , 1979. In the present study, urinary LDH isoenzymes were evaluated in urinary specimens examined by routine cytology. Materials and methodsUrinary specimens collected from routine cytology were centrifuged at 1,500 r.p.m. for 10 min. The method. Simultaneously, air-dried smears were also prepared for Giemsa stain to determine the number of contaminating leucocytes and erythrocytes per high powered field (x 400). Cytological findings were interpreted as negative, suspicious or positive for malignant cells in the Papanicolaou-smears.Thus, a total of 106 specimens which contained less tjhan 20 leucocytes and less than 20 erythrocytes per high power field and were cytologically diagnosed as negative...

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Lactate dehydrogenase isoenzyme patterns in bronchogenic carcinoma

Cited by 39 publications

References 25 publications

Enzymatic characteristics of tubular adenomas and carcinomas of the large intestine.

Enzymatic characteristics of tubular adenomas and carcinomas of the large intestine.

Lactate dehydrogenase 5: An old friend and a new hope in the war on cancer

The diagnostic significance of lactate dehydrogenase isoenzymes in urinary cytology

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