Lactate dehydrogenase activity in the diagnosis of malignant effusions

Horrocks, J. E.; King, John A.; Waind, A. P. B.; Ward, J.

doi:10.1136/jcp.15.1.57

Cited by 17 publications

(9 citation statements)

References 6 publications

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“…Tais achados corroboram os estudos realizados em humanos, que relatam que a atividade de lactato desidrogenase tende a se elevar em efusões transudativas (HORROCKS, et al, 1962;LIGTH, et al, 1972;JOSEPH et al, 2001;ROMERO-CANDEIRA et al, 2002).…”

Section: Resultsunclassified

Correlação da atividade de lactato desidrogenase e concentração de lactato com a classificação de efusões em cães

2011

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RESUMO As efusões são problemas clínicos frequentes e que ocorrem em consequência de uma enfermidade que culmine com diminuição da pressão coloidosmótica intravascular INTRODUÇÃOEm condições normais, um pequeno volume de fluido intracavitário é formado a partir da filtração do plasma pelo endotélio capilar, que ocorre através da interação da pressão hidrostática desses capilares,

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Section: Resultsunclassified

Correlação da atividade de lactato desidrogenase e concentração de lactato com a classificação de efusões em cães

2011

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“…5'-nucleotidase (5'- ND), was estimated by the method Sandra etal 12 and activity expressed as Mmoles/min/mg protein. The methods of Horrocks 13 was adopted for the assay Lactate dehydrogenise (LDH) and activity expressed as/imoles of pyruvate liberated/min/mg protein. The activity of Aspartate Transaminase in tissue sample was assayed by the method of King etal 14 and enzyme activity was expressed in jumoles of pyruvate liberated/min/mg protein.…”

Section: Methodsmentioning

confidence: 99%

Biochemical and cytogenetic analysis of different grades of glioma patients

Palani¹,

Arunkumar²,

Janardhanam³

2010

ANS

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BackgroundGlioma, a neoplasm of neuroglial cells, is the most common type of brain tumor, constituting more than 50% of all brain tumors.PurposeThis report summarizes the current knowledge regarding the clinical utility of biochemical enzyme markers for both diagnostic and therapeutic purposes in different grades of glioma.MethodsSixty patients with different grades of glioma include glioblastoma multiforme (n=20), Anaplastic astrocytoma (n=10). Oligodendroglioma (n=10). Ependymoma (n=10), Pilocytic astrocytoma (n=10) and patients with benign lesions (n=5) served as controls. Activity of antioxidants and marker enzymes were assayed in all grades followed by karyotyping.ResultsActivities of antioxidant enzymes reduced significantly (p<0.05) compared to controls except CAT, GST that showed marked increase (p<0.05) in accordance with malignancy. CK, Na-K+ ATPases, 5'-Nucleotidases showed marked increase in grade IV. Similarly, Mg2-ATPase, Ca2+ATPases showed significant increase in grade III.ConclusionThe clinical importance for classification and treatment of glioma is governed by biochemical enzyme markers. The study of enzymes supported by related chromosomal changes is anticipated to provide better appreciation of biological properties in different grades of glioma.

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“…Lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) were determined by the method of King[13] and Okinaka and his coworker,[14] respectively, using commercially available kit. Blood glucose was measured by the glucose-oxidase method.…”

Section: Methodsmentioning

confidence: 99%

A Novel Approach for Oral Delivery of Insulin via Desmodium gangeticum Aqueous Root Extract

Kurian

Seetharaman

Subramanian

et al. 2010

Journal of Young Pharmacists

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Many challenges are associated with the oral delivery of insulin, relating to the physical and chemical stability of the hormone, and its absorption and metabolism in the human body. The present study aims to demonstrate the oral delivery of insulin in both normal and steptozotocin (STZ)-induced diabetic rats with the help of the aqueous extract of Desmodium gangeticum (DG) root. Human insulin was mixed with the aqueous extract of DG root (0.1 mg/ml) with human insulin (40 IU/ml) in ratio 1:1(v/v), to prepare oral insulin drug. Decreased plasma glucose level and increased plasma insulin in normal and STZ-induced diabetic rat suggested the probable absorption of insulin through GI tract when insulin was administered by mixing with DG extract. Indeed, insulin mixed DG potentially stimulates the release of insulin in STZ-induced diabetic rat rather than in normal animal. In vivo insulin secretaguage action of oral insulin drug was determined by isolated rat heart model and the results showed a significant cardio protection in STZ rat. The finding of this study suggests that insulin mixed with DG extract can be a promising vehicle for oral delivery of insulin. However, further studies are required to explore the exact compound(s) responsible for the protective delivery of insulin orally. Increased plasma insulin level by insulin mixed DG extract administration in STZ-treated diabetic rat indicates not only insulin secretaguage action of the mixture but also a probable altered insulin release mechanism in diabetic condition.

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Lactate dehydrogenase activity in the diagnosis of malignant effusions

Cited by 17 publications

References 6 publications

Correlação da atividade de lactato desidrogenase e concentração de lactato com a classificação de efusões em cães

Correlação da atividade de lactato desidrogenase e concentração de lactato com a classificação de efusões em cães

Biochemical and cytogenetic analysis of different grades of glioma patients

A Novel Approach for Oral Delivery of Insulin via Desmodium gangeticum Aqueous Root Extract

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