Biochemical and cytogenetic analysis of different grades of glioma patients

Abstract

BackgroundGlioma, a neoplasm of neuroglial cells, is the most common type of brain tumor, constituting more than 50% of all brain tumors.PurposeThis report summarizes the current knowledge regarding the clinical utility of biochemical enzyme markers for both diagnostic and therapeutic purposes in different grades of glioma.MethodsSixty patients with different grades of glioma include glioblastoma multiforme (n=20), Anaplastic astrocytoma (n=10). Oligodendroglioma (n=10). Ependymoma (n=10), Pilocytic astrocytoma (n=10) and patients with benign lesions (n=5) served as controls. Activity of antioxidants and marker enzymes were assayed in all grades followed by karyotyping.ResultsActivities of antioxidant enzymes reduced significantly (p<0.05) compared to controls except CAT, GST that showed marked increase (p<0.05) in accordance with malignancy. CK, Na-K+ ATPases, 5'-Nucleotidases showed marked increase in grade IV. Similarly, Mg2-ATPase, Ca2+ATPases showed significant increase in grade III.ConclusionThe clinical importance for classification and treatment of glioma is governed by biochemical enzyme markers. The study of enzymes supported by related chromosomal changes is anticipated to provide better appreciation of biological properties in different grades of glioma.

Comparative analysis of antioxidative systems in malignant and benign brain tumours

“…30 In addition, two comparative studies on meningioma and astrocytoma found that malignant brain tumours show a lower activity of GSH-Px (the rank order was: meningioma > low-grade astrocytoma > high-grade astrocytoma), 31 as well as lower glutathione concentration and SOD activity. 31,32 It is noteworthy that Palani et al 33 have shown that GSH-Px, GR, and SOD activity in gliomas gradually decrease with increasing tumour grade. In discrepancy to our findings, Pu et al 31 have found higher CAT activity in meningioma compared to high-grade astrocytoma.…”

Revising Fourier-transform infrared (FT-IR) and Raman spectroscopy towards brain cancer detection

“…For example, the activation of the polyamine metabolism is closely related with cellular proliferation [38]. Brain cancers show particular complexity despite some advances in discovering genetic and molecular features about this pathology [39], as an example, the chromosome complete or partial loss of the tumour suppression gene or enzymes associated to various signaling mechanism for the cell cycle control [40]. Several studies using both FT-IR and Raman spectroscopy have found spectral markers associated with tumour appearance and progression, as listed in Tables 2 and 3.…”