Laccase immobilization over multi-walled carbon nanotubes: Kinetic, thermodynamic and stability studies

Tavares, Ana P. M.; Silva, Cláudia G.; Dražić, Goran; Silva, Adrián M.T.; Loureiro, José M.; Faria, Joaquim L.

doi:10.1016/j.jcis.2015.04.054

Cited by 191 publications

(90 citation statements)

References 58 publications

(53 reference statements)

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“…The half‐life of the enzyme in the nanoflower, at 80 °C is almost 25 folds higher than in the free form. The nanoflower probably stabilized the vibration of the atoms in the protein caused by higher temperature, thereby preventing drastic changes in the three dimensional structure of the lipase and maintaining the enzyme in active form . A quantitative evaluation of the temperature effect was done through the Arrhenius plot, in order to determine the activation energy of the enzyme in free and immobilized form (supporting information, Figure S1).…”

Section: Resultsmentioning

confidence: 99%

An Ultrafast Sonochemical Strategy to Synthesize Lipase‐Manganese Phosphate Hybrid Nanoflowers with Promoted Biocatalytic Performance in the Kinetic Resolution of β‐Aryloxyalcohols

2018

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Herein we report an expeditious and facile sonochemical synthesis method that takes just a few minutes to produce organic‐inorganic hybrid nanoflowers and their application as a nanobiocatalyst. Surfactant treated Burkholderia cepacia lipase was used as the organic component and manganese sulfate as the inorganic component to synthesize the nanoflowers in less than 10 minutes. Careful investigation of the process parameters was performed to obtain the optimized value for each attribute viz. sonication time (7.5 min), lipase concentration (0.1 mg/mL), MnSO4 concentration (150 mM), sonication frequency (33 kHz) and pH (8.0). An additional post‐synthesis treatment of nanoflowers with N‐hydroxysuccinimide (NHS) increased their reusability to over nine cycles with an overall six‐fold increase in the activity. In addition, thermal stability was demonstrated through a twenty‐fold increase in the half‐life of immobilized lipase at 80 °C than in free form. The hybrid nanoflowers displayed a marked improvement in the kinetic resolution of racemic intermediates of metoprolol, a selective β‐1 blocker drug [C (%)=46.7, eep=96.5, E=156] and cloranolol, a non‐selective β‐blocker drug [C (%)=49.5, eep=96.0, E=157] compared to free lipase which signifies its importance as a nanobiocatalyst.

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Section: Resultsmentioning

confidence: 99%

An Ultrafast Sonochemical Strategy to Synthesize Lipase‐Manganese Phosphate Hybrid Nanoflowers with Promoted Biocatalytic Performance in the Kinetic Resolution of β‐Aryloxyalcohols

2018

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“…The FTIR spectrum typical laccase (see (b)) shows five main bands: (1) a strong band centered at 3300 cm −1 due to O–H and NH vibrations; (2) weak bands at around 2800 cm −1 attributed to CH bonds; (3) a band corresponding to the NH peptide linkage at 1630 cm −1 ; (4) a band peaking at 1350 cm −1 attributed to the CN stretching vibration of amines; and (5) a strong and sharp band peaking at 1100 cm −1 due to COC [35,36,37,38]. …”

Section: Resultsmentioning

confidence: 99%

Electrochemical Study and Characterization of an Amperometric Biosensor Based on the Immobilization of Laccase in a Nanostructure of TiO2 Synthesized by the Sol-Gel Method

2016

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Laccase amperometric biosensors were developed to detect the catechol compound. The laccase enzyme (LAC) immobilization was performed on nanostructures of (a) titania (TiO2); (b) titania/Nafion (TiO2/NAF) (both immobilized by the sol-gel method) and a third nanostructure, which consisted of a single biosensor composite of Nafion and laccase enzyme denoted as NAF/LAC. The Nafion was deposited on a graphite electrode and used to avoid “cracking” on the matrix. The TiO2 particle size was an average of 66 nm. FTIR spectroscopy vibration modes of different composites were determined. The electrochemical behavior of the biosensor was studied using electrochemical spectroscopy (EIS) and cyclic voltammetry (CV). The biosensor based on TiO2/NAF/LAC presented the best electro-chemical properties with regard to sensitivity, stability and detection limit after a period of 22 days.

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“…Immobilization of CRL would extend reaction life and lead to the excellent binding capacity, favorable physicochemical properties as well as biological compatibility of MWCNTs (Zhang and Henthorn, 2010). The role of the multiwalled carbon nanotubes (MWCNTs) as support materials in enhancing activity and stability of enzymes has been clearly indicated in literature (Tavares et al, 2015). The improved stability of immobilized enzymes is due to the formation of stable multipoint interactions between lipase molecules and support materials (Metin, 2013).…”

Section: Introductionmentioning

confidence: 99%

Sustainable production of the emulsifier methyl oleate by Candida rugosa lipase nanoconjugates

Marzuki

Mahat

Huyop

et al. 2015

Food and Bioproducts Processing

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Laccase immobilization over multi-walled carbon nanotubes: Kinetic, thermodynamic and stability studies

Cited by 191 publications

References 58 publications

An Ultrafast Sonochemical Strategy to Synthesize Lipase‐Manganese Phosphate Hybrid Nanoflowers with Promoted Biocatalytic Performance in the Kinetic Resolution of β‐Aryloxyalcohols

An Ultrafast Sonochemical Strategy to Synthesize Lipase‐Manganese Phosphate Hybrid Nanoflowers with Promoted Biocatalytic Performance in the Kinetic Resolution of β‐Aryloxyalcohols

Electrochemical Study and Characterization of an Amperometric Biosensor Based on the Immobilization of Laccase in a Nanostructure of TiO2 Synthesized by the Sol-Gel Method

Sustainable production of the emulsifier methyl oleate by Candida rugosa lipase nanoconjugates

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