Sustainable production of the emulsifier methyl oleate by Candida rugosa lipase nanoconjugates

Marzuki, Nur Haziqah Che; Mahat, Naji Arafat; Huyop, Fahrul; Aboul‐Enein, Hassan Y.; Wahab, Roswanira Ab.

doi:10.1016/j.fbp.2015.08.005

Cited by 33 publications

(16 citation statements)

References 47 publications

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“…The results were supportive of butyl butyrate being produced by the CRL/G/A/SiO2-M catalyzed esterification reaction. 1 H NMR spectrum of ester produced in the CRL/G/A/SiO2-M catalyzed the esterification of 1-butanol, and butyric acid depicted 16 protons that were characteristic for butyl butyrate (CAS: 109-21-7) ( Fig. 5).…”

Section: Ftir Spectroscopymentioning

confidence: 99%

“…The reasons behind this have to do with its well-reported good stereospecificity, high enantioselectivity, and stability in organic solvents, as well as possessing good thermal stability, the capability of catalyzing a plethora of important industrial reactions. Among these reactions, CRL catalyzes several reactions including hydrolysis, transesterification, esterification, and interesterification which require mild optimal working conditions through an energy-saving route [1][2][3][4]. However, like any other forms of free enzymes, the free form of CRL is vulnerable to deactivation under harsh industrial settings [5].…”

Section: ■ Introductionmentioning

confidence: 99%

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Effect of Glutaraldehyde Concentration on Catalytic Efficacy of <i>Candida rugosa</i> Lipase Immobilized onto Silica from Oil Palm Leaves

Onoja

Wahab

2019

Indones. J. Chem.

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Till date, studies that investigated the effect of glutaraldehyde concentration on catalytic efficacy of biocatalyst developed with silica-derived from oil palm leaves (OPL) as support, are unknown. The study presents the preparation of a support consisting of silica extracted from OPL coated over magnetite (G/A/SiO2-M) for the immobilization of Candida rugosa lipase (CRL). Herein, the effect of glutaraldehyde concentration on the catalytic efficacy of immobilized CRL was assessed by the enzymatic production of butyl butyrate as a model. Fourier transform infrared (FTIR) spectra and immobilization parameters indicated that covalent bound CRL on functionalized OPL-derived silica-magnetite composite activated with 4% (v/v) glutaraldehyde solution (100 mM, pH 7.0) (CRL/G/A/SiO2-M) and pretreated in toluene, resulted in a protein loading and an immobilization yield of 68.3 mg/g and 74.3%, respectively. The resultant CRL/G/A/SiO2-M biocatalyst which specific activity was 61.9 U/g catalyzed the esterification production of 76.5% butyl butyrate in just 3 h, as confirmed by analyses of the purified ester using FTIR and 1H NMR spectroscopy. Hence, the finding envisages the promising use of G/A/SiO2-M support fabricated from discarded OPL as a carrier for immobilization and activation of CRL, in conjunction to being a good alternative source of renewable silica.

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Section: Ftir Spectroscopymentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Effect of Glutaraldehyde Concentration on Catalytic Efficacy of <i>Candida rugosa</i> Lipase Immobilized onto Silica from Oil Palm Leaves

Onoja

Wahab

2019

Indones. J. Chem.

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“…[14] More importantly,t he solid emulsifier can also act as an efficient support for the immobilization of catalysts, with good reusability just like many other supported catalysts. [15] In brief, the Janus material can not only be used as ar eusable emulsifiert oa chieve the emulsive catalytic reactionw ith as implified demulsification process, but can also serve as ac arrierf or the catalyst after suitable modification, which leads to an enrichmento fc atalystp resent at the liquid interface. Therefore, if ECODSi sp erformed with this type of Janus emulsion, the enhancement of sulfur removal efficiency and the reuse of both the emulsifier and catalystc an be achieved.…”

Section: Introductionmentioning

confidence: 99%

Catalytic Emulsion Based on Janus Nanosheets for Ultra‐Deep Desulfurization

Xia

Zhang

Zhou

et al. 2017

Chemistry A European J

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Catalytic Janus nanosheets were synthesized by using an anion-exchange reaction between heteropolyacids (HPAs) and the modified ionic-liquid (IL) moieties of Janus nanosheets. Their morphology and surface properties were characterized by using SEM, energy-dispersive spectroscopy (EDS), FTIR spectroscopy, and X-ray photoelectron spectroscopy (XPS) studies. Because of their inherent Janus structure, the nanosheets exhibited good amphipathic character with ILs and oil to form a stable ILs-in-oil emulsion. Therefore, these Janus nanosheets can be used as both emulsifiers and catalysts to perform emulsive desulfurization. During this process, sulfur-containing compounds at the interface could be easily oxidized and efficiently removed from a model oil. Application of this Janus emulsion brings an efficient, useful, and green procedure to the desulfurization process. Compared with the desulfurization catalyzed by using HPAs in a conventional two-phase system, the sulfur removal of dibenzothiophene (DBT) achieved in a Janus emulsion system was improved from 68 to 97 % within 1.5 h. Moreover, this emulsion system could be demulsified easily by simple centrifugation to recover both the nanosheets and the ILs. Owing to the good structural stability of the Janus nanosheets, the sulfur removal efficiency of DBT could still reach 99.9 % after the catalytic nanosheets had been recycled at least six times.

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“…Moreover, manufacturing processes that use natural enzymes as catalysts are typically carried out under near ambient conditions i.e. (1 atm, 30-50 o C, pH 6-8) (KARBOUNE et al, 2008;MARZUKI et al, 2015a;MARZUKI et al, 2015b;MOHAMAD et al, 2015b;MARZUKI et al, 2015c;MANAN et al, 2016;KHALILI et al, 2017), similar to conditions under agricultural settings. For these reasons, an enzyme-based fungicide i.e.…”

Section: Introductionmentioning

confidence: 99%

Assessments on the catalytic and kinetic properties of beta-glucosidase isolated from a highly efficient antagonistic fungus Trichoderma harzianum

Khalili¹,

Huyop²,

Javed³

et al. 2018

Biosci. J.

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Due to the toxicity and inefficiency of chemical fungicides to control infestation of Macrophomina phaseolina (Tassi) Goid which causes charcoal rot in plants, a biotechnological approach using βglucosidase (EC.3.2.1) as the alternative bioactive ingredient in fungicide is hereby, proposed. The extracellular enzyme was isolated from a highly efficient fungal antagonist, Trichoderma harzianum T12. The highly similar molecular masses obtained using SDS-PAGE (96 kDa) and MALDI-TOF mass spectrometry (98.3 kDa) affirmed that the β-glucosidase was purified to homogeneity. Consequently, optimum catalytic parameters that rendered the highest enzyme activity were found to be: 45˚C, pH 7, inoculum size of 10 % (w/v), supplementation with metal ions Zn 2+ and Mn 2+ ions, and Tween 80. Addition of wheat bran and (NH 4 ) 2 SO 4 as carbon and nitrogen sources also improved enzyme activity. BLASTn showed the sequence of β-glucosidase T12 was highly identical to other β-glucosidases viz. T. harzianum strain IOC-3844 (99%), T. gamsii and T. virens bgl1 (86 %) as well as T. reesei strain SJVTR and T. viride strain AS 3.3711 (84 %). Kinetic assessment showed that β-glucosidase T12 catalyzes hydrolytic activity is characterized by a K m of 0.79 mM and V max of 8.45 mM min -1 mg -1 protein, with a corresponding k cat of 10.69 s -1 .

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Sustainable production of the emulsifier methyl oleate by Candida rugosa lipase nanoconjugates

Cited by 33 publications

References 47 publications

Effect of Glutaraldehyde Concentration on Catalytic Efficacy of <i>Candida rugosa</i> Lipase Immobilized onto Silica from Oil Palm Leaves

Effect of Glutaraldehyde Concentration on Catalytic Efficacy of <i>Candida rugosa</i> Lipase Immobilized onto Silica from Oil Palm Leaves

Catalytic Emulsion Based on Janus Nanosheets for Ultra‐Deep Desulfurization

Assessments on the catalytic and kinetic properties of beta-glucosidase isolated from a highly efficient antagonistic fungus Trichoderma harzianum

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