Laboratory Typing Methods for Diagnostic of Salmonella Strains, the “Old” Organism That Continued Challenges

Imen, Ben Salem; Mzoughi, Ridha; Aouni, Mahjoub

doi:10.5772/29010

Cited by 3 publications

(2 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Salmonella has been the primary cause of the foodborne salmonellosis pandemic in humans over the last 20 years, during which egg products were the most often identified vehicle of the infection (Moosavy et al, 2015;Long et al, 2017). Over the past decades, the improper and uncontrolled use of antibiotics has allowed the appearance of strains of Salmonella with multiple drug resistance in many countries (Ben Salem et al, 2012;Nacer et al, 2022;Samy et al, 2022). For that reason, the antimicrobial resistance of Salmonella has become a matter of concern worldwide due to the significant threat that can present to public health (Wu and Hulme, 2021).…”

Section: Discussionmentioning

confidence: 99%

Detection and Antimicrobial Susceptibility of Salmonella spp. Isolated From Commercial Eggs in Tiaret Province, Algeria

Merati¹,

Boudra²

2023

WVJ

View full text Add to dashboard Cite

Salmonellosis is a significant public health problem worldwide. The current study aimed to investigate the presence of Salmonella spp. in commercial eggs of Tiaret province, Algeria, and evaluate the susceptibility of isolated strains to different antimicrobial agents. A total of 180 commercial eggs collected from various retail outlets (groceries, butchers, wholesalers, street vendors) were analyzed by conventional methods, and 13 Salmonella spp. isolates were tested on a panel of 7 antimicrobial agents using the disc diffusion method. Of 180 chicken egg content samples examined, the findings indicated that 13 (7.22%) were positive for Salmonella spp. Regarding the collection site, 2 (1.11%), 4 (2.22%), and 7 (3.88%) of Salmonella spp. isolates were detected from butchers, wholesalers, and street vendors, respectively. Most antibiotic discs have demonstrated widespread resistance with an incidence rate of 100%, including amoxicillin + clavulanic acid, ampicillin, nalidixic acid, and erythromycin. However, colistin sulfate, gentamycin, and tetracycline were more effective against Salmonella isolates. It can be concluded that the highest detection rate of Salmonella spp. was observed for street vendors, and the highest resistance was recorded for commonly used antibiotics in poultry production.

show abstract

Section: Discussionmentioning

confidence: 99%

Detection and Antimicrobial Susceptibility of Salmonella spp. Isolated From Commercial Eggs in Tiaret Province, Algeria

Merati¹,

Boudra²

2023

WVJ

View full text Add to dashboard Cite

show abstract

“…The confirmation of Salmonella spp. were analysed according to Ben Salem et al [8] with some modifications. From each sample, 25 g was pre-enriched in 275 ml buffered peptone water (HIMEDIA, INDIA) at 37°C for 24h.…”

Section: Isolation and Biochemical Characterization Of The Isolatesmentioning

confidence: 99%

Isolation and identification of pathogenic bacteria from ready-to-eat fast foods in Al-Quwayiyah, Kingdom of Saudi Arabia

Alharbi¹,

Abdel-Ghaffar²,

Kadher³

2019

AJFAND

View full text Add to dashboard Cite

Food-borne pathogens are becoming a globally formidable health problem and perceived as a major health concern in the Kingdom of Saudi Arabia (KSA). Contamination ensued through unclean raw food materials and particles, use of polluted water, unhygienic preparation processes and use of contaminated containers. Herein, the prevalence of food-borne pathogens in ready-to-eat (RTE) fast foods from fifteen different food eateries such as 7 restaurants, 6 cafeterias and 2 two college canteens in Al-Quwayiyah, Riyadh Region of Saudi Arabia was studied. Microbiological analysis of 155 fast food samples which included, Vegetable salad, Falafel, Kibtha and Shawarma. The isolates were detected using biochemical tests and API 20E and slide agglutination test were conducted for Salmonella spp. detection. Bacterial growth was found in all food samples tested. Moreover, the test also showed high levels of total aerobic count: vegetable salad 6.34+0.03, falafel 5.79 + 0.18, kibtha 5.06 +0.02 and shawarma 3.54 + 0.13. Organisms isolated include Salmonella spp. (15%), Escherichia coli (18%) and Staphylococcus aureus (7%). Salmonella is one of the most virulent pathogen implicated in food-borne disease outbreaks. There are numerous transmission routes for Salmonellosis, but the majority of the human infections are derived from consumption of contaminated poultry products. Consistently, Salmonella Enteritidis, Salmonella Typhimurium and Salmonella Heidelberg are the three most frequent serotypes recovered from humans each year. Serologically identified Salmonella serotypes from RTE fast food samples were Salmonella Typhimurium with 65%, the most predominant one compared to Salmonella Enteritidis that was 35%. The bacterial count of vegetable salad, falahfel, kibtha was statistically significant when compared with Shawarma (p < 0.05). This result indicated that most of the ready-to-eat food samples examined in the study did not meet any bacteriological quality standard as recommended by The New South Wales (NSW) Food Authority to be <5.0 log10 CFU g−1 and, therefore, it poses potential risks to consumers. Ready-to-eat fast foods must be cooked and served to the consumers with all hygienic measures.

show abstract

Application of RAPD-PCR and Phylogenetic Analysis for Accurate Characterization of Salmonella spp. Isolated from Chicken and Their Feed and Drinking Water

Hasan¹,

Lafta

Ahmed

et al. 2023

Iraqi J. Vet. Med.

View full text Add to dashboard Cite

The aim of this study was ‎the‎ discrimination of Salmonella‎‎ isolated from chicken and their feed ‎and drinking water for the epidemiological control of salmonellosis. Totally, 289 samples, ‎including 217 chicken cloaca swabs, 46 water, and 26 feed samples were collected from five ‎different farms in Karbala governorate, Iraq. Conventional bacteriology tests, API 20E, Vitek 2, ‎and serology were used for bacterial identification. Random amplified polymorphic ‎DNA (RAPD)-polymerase chain reaction (PCR) was applied to analyze the genetic relationships ‎among Salmonella‎‎ isolates. The isolation rate of Salmonella‎‎ spp. was 21.1% (61/289). While the ‎water samples constituted the highest rate (30.4%), a rate of 21.7% was reported for the cloaca ‎swabs, with no isolate at all from chicken feed. Vitek 2 was able to identify some isolates to the ‎serotype level, such as S. Enteritidis, S. Paratyphi B, and S. Paratyphi C. However, the isolates ‎were diagnosed as S. enterica by API 20E, and as S. enterica subsp. arizonae through serology. ‎Analyzing the samples by the RAPD-PCR assay showed the presence of genetically different ‎Salmonella‎‎ spp. Dendrograms created by the GelJ software successfully delineated the genetic ‎relationships. Therefore, RAPD-PCR can be used as a surrogate tool for the fast, reliable, and ‎accurate detection of Salmonella‎‎ in epidemiological surveys when compared with other ‎biochemical-based identification methods.

show abstract

Laboratory Typing Methods for Diagnostic of Salmonella Strains, the “Old” Organism That Continued Challenges

Cited by 3 publications

References 85 publications

Detection and Antimicrobial Susceptibility of Salmonella spp. Isolated From Commercial Eggs in Tiaret Province, Algeria

Detection and Antimicrobial Susceptibility of Salmonella spp. Isolated From Commercial Eggs in Tiaret Province, Algeria

Isolation and identification of pathogenic bacteria from ready-to-eat fast foods in Al-Quwayiyah, Kingdom of Saudi Arabia

Application of RAPD-PCR and Phylogenetic Analysis for Accurate Characterization of Salmonella spp. Isolated from Chicken and Their Feed and Drinking Water

Contact Info

Product

Resources

About