Laboratory diagnosis of urinary tract infections using diagnostics tests in adult patients

Sk, Akmal Hasan; T, Naveen Kumar; N, Radha Kishan; Neetha, K

doi:10.5455/2320-6012.ijrms20140508

Cited by 8 publications

(4 citation statements)

References 12 publications

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“…Although all the study subjects were clinically diagnosed with UTI, the prevalence of culture-positive urine specimens was as low as 10%. By comparison, similar studies in India and the US reported that 45% and 50%, respectively, of patients clinically diagnosed with UTI had culture-positive urine 51,52. The disparity between clinical and laboratory (culture) diagnosis of UTI can be partly attributed to non-specific clinical symptoms, which is a common problem in the diagnosis of the infection.…”

Section: Discussionmentioning

confidence: 95%

<p>Community acquired urinary tract infections among adults in Accra, Ghana</p>

Donkor¹,

Horlortu²,

Dayie³

et al. 2019

IDR

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Background: Urinary tract infection (UTI) is one of the most common bacterial infectious diseases encountered in clinical practice, and accounts for significant morbidity and high medical costs. To reduce its public health burden, there is the need for local research data to address aspects of prevention and management of UTI. The aim of this study was to investigate community-acquired UTI among adults in Accra, Ghana, including the risk factors, etiological agents, and antibiotic resistance. Methods: This was a cross-sectional study involving 307 patients clinically diagnosed with UTI at the Korle Bu and Mamprobi polyclinics in Accra. Urine specimens were collected from the study participants and analyzed by culture, microscopy, and dipstick. The bacterial isolates were identified using standard microbiological methods and tested against a spectrum of antibiotics by the Kirby Bauer method. Multidrug resistant Enterobacteriaceae isolates were screened for Extended Spectrum β-lactamase (ESBL) production by the double disc method, and isolates that tested positive were analyzed by Polymerase Chain Reaction for ESBL genes. Demographic information and clinical history of study participants were collected. Results: Based on the criteria for laboratory confirmed UTI, 31 (10.1%) of the 307 specimens were positive and the main risk factor of UTI among the study participants was pregnancy ( P =0.02, OR=2.43). The most common uropathogen isolated was Escherichia coli (48.9%), followed by Klebseilla sp. (16.1%). Prevalence of resistance was highest for Piperacillin (87.1%) and Amoxicillin+Clavulanic Acid (87.1%) and lowest for Amikacin (12.9%). Prevalence of multidrug resistance among the uropathogens was 80.1% (25) and the most common ESBL gene detected was CTX-M-15. Conclusion: Pregnant women constitute the key risk population of UTI in Accra, while Amikacin remains a suitable drug for the treatment of febrile UTI. The high prevalence of multidrug resistance among the uropathogens highlights the need for surveillance of antimicrobial resistance among these pathogens.

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Section: Discussionmentioning

confidence: 95%

<p>Community acquired urinary tract infections among adults in Accra, Ghana</p>

Donkor¹,

Horlortu²,

Dayie³

et al. 2019

IDR

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“…samples during catheter insertion (Akmal-Hasan et al, 2014), especially in the case of permanent catheters. Hence, the best option for the collection of urine samples in studies regarding the urinary microbiome is suprapubic aspiration, since it is performed directly from the bladder avoiding the contact with other areas.…”

Section: The Evolution Of Urinary Microbiome Analysis: Methodology and Limitationsmentioning

confidence: 99%

Urinary Microbiome: Yin and Yang of the Urinary Tract

Pérez-Carrasco

Soriano‐Lerma

Soriano

et al. 2021

Front. Cell. Infect. Microbiol.

121

119

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The application of next generation sequencing techniques has allowed the characterization of the urinary tract microbiome and has led to the rejection of the pre-established concept of sterility in the urinary bladder. Not only have microbial communities in the urinary tract been implicated in the maintenance of health but alterations in their composition have also been associated with different urinary pathologies, such as urinary tract infections (UTI). Therefore, the study of the urinary microbiome in healthy individuals, as well as its involvement in disease through the proliferation of opportunistic pathogens, could open a potential field of study, leading to new insights into prevention, diagnosis and treatment strategies for urinary pathologies. In this review we present an overview of the current state of knowledge about the urinary microbiome in health and disease, as well as its involvement in the development of new therapeutic strategies.

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“…Therefore, in order to isolate and detect urinary tract infections in patients with negative aerobic and overnight culture results, especially if they are associated with the presence of symptoms, it is necessary to culture the samples in additional and selective environments with changes in the incubation conditions for isolating the bacteria. On the other hand, Hasan et al (17) confirmed that for an effective treatment, physicians should distinguish urinary tract infections caused by different organisms. In addition, appropriate clinical information could provide clues for better diagnostic evaluation and determination of susceptibility to antimicrobial agents as well addressing host factors that contribute to the occurrence of infection.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Relationship Between Clinical Symptoms and Isolation of Fastidious Bacteria in Urine Samples of Elderly Females

Soleimani

Mousavi

2018

Int J Infect

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Background: Although urinary tract infections (UTIs) are one of the common infections, especially among elderly, the gold standard diagnostic method is still bacterial culture. Since large percentages of urine culture are negative, it seems that specialists should pay more attention to other bacteria, such as fastidious bacteria. The aim of this study was to use the method of fastidious bacteria isolation for urine samples of elderly females with negative results for conventional culture. Methods: This study was conducted at Imam Khomeini hospital and fastidious bacteria from urine samples of 1075 females older than 60 years were isolated and identified. Developing changes in culture media and incubation conditions and gas levels led to isolation of fastidious bacteria. Results: After overnight aerobic culturing of 1075 urine samples, 383 cases had positive, 99 cases had low counting, 159 cases had mixed, and 434 cases had negative test results. Among 434 negative cases obtained from these methods, and using fastidious bacteria in this study, 147 subjects had Fastidious bacterial, more than 50% of which was associated with infection symptoms, indicating a significant difference. Conclusions: For the isolation and detection of urinary tract infections in elderly female patients with negative aerobic and night culture results, especially if it was associated with the presence of symptoms, culturing the samples in additional and selective environments with changes in the incubation conditions is necessary in order to isolate bacteria.

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Laboratory diagnosis of urinary tract infections using diagnostics tests in adult patients

Cited by 8 publications

References 12 publications

<p>Community acquired urinary tract infections among adults in Accra, Ghana</p>

<p>Community acquired urinary tract infections among adults in Accra, Ghana</p>

Urinary Microbiome: Yin and Yang of the Urinary Tract

Evaluation of the Relationship Between Clinical Symptoms and Isolation of Fastidious Bacteria in Urine Samples of Elderly Females

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