Labisia pumila improves wound healing process in ovariectomized rat model

Abstract

Labisia pumila (Blume) Fern.-Vill. synonym Marantodes pumilum (Blume) Kuntze is a flowering plant of the Primulaceae family. It is used traditionally by the Malay women to shrink the uterus, facilitate labor, improve menstrual irregularities and as post-partum medicine (Runi, 2000). The ethnopharmacological studies and clinical findings have illustrated that L. pumila possesses many biological functions, including phytoestrogenic, antimicrobial, anti-oxidative, anti-inflammatory properties and anti-aging (Choi et al., 2010;Nadia et al., 2012). Recent studies on L. pumila also demonstrated that it could also be used for osteoporosis, cardiovascular diseases, and metabolic disorders (Shuid et al., 2011). The ethnobotanical and therapeutic properties of L. pumila make it a potential source of novel medicine for the healing of wounds. Therefore, this study investigates the effectiveness of topical application of the aqueous extract of L. pumila on the rate of wound closure and the histology of the wound area. Materials and Methods Plant collection and extractionThe plant species were collected from their natural habitat in the rainforest of Taiping, Perak, Malaysia in October, 2014. Identification of the plant species was verified by a botanist from the Faculty of Science, Universiti Kebangsaan Malaysia (UKM). All voucher specimens were deposited in the Herbarium of UKM (voucher specimen number of L. pumila var. alata = UKMB 30006/SM 2622 and var. pumila = UKMB 30007/ SM s.n.). The standardized aqueous extraction method was used according to Jamal et al. (2003) AbstractThe purpose of this study was to determine the wound healing effect of Labisia pumila in the ovariectomized rat model. Ninety-nine Sprague Dawley female rats equally divided into nine groups; where five were control groups, and four were treated groups. The dressing was changed daily, starting from the wound induction until complete healing. The percentage of wound contraction was measured on day 0, 2, 5, 8, 9, 10, 11, 12 and 13. Three rats were sacrificed from each group on day 2, 5 and 8 respectively for evaluating of histological assessments. Wounds dressed with extract showed considerable healing and significantly healed faster compared to all control groups (p<0.05). Moreover, histological analysis revealed remarkable reduction in the scar width correlated with the enhanced collagen content and fibroblast cells, accompanied by a reduction of inflammatory cells in the granulation tissues.In conclusion, L. pumila may promote wound healing in postmenopausal rat model. Article Info

Evaluation of Wound-Healing and Antioxidant Effects of Marantodes pumilum (Blume) Kuntze in an Excision Wound Model

“…The changes in wound size were calculated to determine the day the wound healed based on the equation by Ahmad, S.U. et al [ 24 ].…”

“…The aqueous extract of MP inhibited estradiol binding with the estrogen receptor of antibodies and suggested the existence of estrogen-like compounds [ 23 ]. In our previous study, the phytoestrogenic properties of MP were postulated to promote wound healing [ 24 ]. However, it was shown that there was no significant difference between the estrogen-treated and normal healing processes in an ovariectomized rat model.…”

The effects of aqueous extract of Labisia Pumila (Blume) Fern.-Vill. Var. Alata on wound contraction, hydroxyproline content and histological assessments in superficial partial thickness of second-degree burn model

“…This emulsifying ointment was chosen as a vehicle as it is chemically inert and inactive to the skin ( Ahmad et al, 2021 ). A 2.0% dose concentration was chosen for both the leaf and root extracts as previously established by Ahmad et al (2018) using LP extracts on minor wound healing ( Ahmad et al, 2018 ). The extracts were crushed using a pestle and mortar to obtain fine powder which have faster absorption rates and better uniformity when mixed with emulsifying ointment.…”

“…In a previous study by Choi et al (2010) , LP extract demonstrated protection of skin against photoaging induced by ultraviolet irradiation ( Choi et al, 2010 ). Meanwhile, a study by Ahmad et al (2018) reported that LP extract promoted minor wound healing in ovariectomized rat model ( Ahmad et al, 2018 ). However, the effects of LP on burn wound healing have not been studied yet.…”

Plant-based Bone Grafting: The Future of Bone Healing

“…Plants used in the fractures treatment must have important characteristics through their active constituents, presenting the following activities: antimicrobial; antioxidant, important in free radical scavenging that contribute to bone healing delay; osteogenic activity, contributing to increased osteoblast proliferation, osteocytes and osteoclasts; angiogenic activity, acting on the supply of nutrients to the fracture bed, stimulating collagen production; estrogenic activity, important in cases of fractures caused by metabolic diseases as osteoporosis [29][30][31] ; anti-inflammatory, through edema control and pain relief by the chemical mediators production, such as arachidonic acid metabolites and cytokines [32] .…”