Labelling and binding of poly-(l-lysine) to functionalised gold surfaces. Combined FT-IRRAS and XPS characterisation

Yam, Chi-Ming; Liu, Zheng; Salmain, Michèle; Pradier, Claire‐Marie; Marcus, Philippe; Jaouen, Gérard

doi:10.1016/s0927-7765(00)00215-0

Cited by 25 publications

(20 citation statements)

References 28 publications

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“…The C 1s spectrum was curvefitted into three different peaks through XPS measurements with an equal full width at halfmaximum (FWHM) using a Shirley-type background (Fig. 4d), and the binding energies at about 284.8, 286.1 and 288.9 eV are attributable to the C-C/C-H, C-O/C-N and C ¼O groups of PEG and PLL, respectively [30]. The peak at 531.6 eV resolved from O1s spectrum could be assigned to O¼ C-O in PEG-COOH and PLL; The peak at 532.8 eV can be assigned to C-O-C and C-O-H [31].…”

Section: Resultsmentioning

confidence: 99%

Preparation of polylysine-modified superparamagnetic iron oxide nanoparticles

Gao

Zhang

Wang

et al. 2015

Journal of Magnetism and Magnetic Materials

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Section: Resultsmentioning

confidence: 99%

Preparation of polylysine-modified superparamagnetic iron oxide nanoparticles

Gao

Zhang

Wang

et al. 2015

Journal of Magnetism and Magnetic Materials

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“…Analytical techniques, including FTIR and AFM, have shown the existence of monolayers and morphorgy of the surface modifications (Ulman 1996;Smith et al 2004;Chidsey and Loiacono 1990;Yam et al 2001;Liao et al 2005;Morales-Cruz et al 2005). Because PDL and PLL are optical isomers, the FTIR results of PLL are good candidates for assigning the specific bondings during PDL immobilizations (Yam et al 2001). According to our FTIR analysis, the exposed COOH functional groups of the MUA monolayer were bonded to PDL by amide covalent bonding after immersing the sample in a solution containing PDL.…”

Section: Discussionmentioning

confidence: 99%

“…Among common approaches, Fourier transform infrared spectroscopy (FTIR) provides the optical approach for examining functional groups and molecular orientation (Liao et al 2005;Ulman 1996;Smith et al 2004;Chidsey and Loiacono 1990;Yam et al 2001). In addition, an atomic force microscope (AFM) was used to assess layer thickness and molecular packing in microscale (Smith et al 2004;Morales-Cruz et al 2005).…”

Section: Analytical Techniques For Surface-modified Meamentioning

confidence: 99%

Characterization of surface modification on microelectrode arrays for in vitro cell culture

Lin¹,

Chen²,

Liao³

et al. 2007

Biomed Microdevices

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This study aims to investigate surface-modified microelectrodes on the microelectrode arrays (MEAs) for neuronal interfaces with in vitro cell culture. The polyimide (PI) MEA was fabricated by using micro-electro-mechanical systems (MEMS) techniques. Self-assembled monolayers (SAMs) of 11-mercaptoundecanoic acid (MUA) were utilized to modify the microelectrode surface of the MEA. The SAMs' modified surface of microelectrodes offered a reliable interface to immobilize biological ligands through covalent bonding. To increase biocompatibility, the poly-D-lysine (PDL) was immobilized on the SAMs' modified microelectrodes. Several analytical techniques were used to define the physical structure and functional groups of surface-modified gold microelectrodes on the MEA. Spectra of the Fourier transform infrared reflection (FTIR) were applied to characterize the molecular structure of MUA-SAMs and PDL on the microelectrodes. The spectra, two peaks of amide I (at 1,613 cm(-1)) and amide II (at 1,548 cm(-1)), revealed that covalent amide bonding existed in PDL-MUA-SAMs modified surfaces. The thickness and formation of the MUA and PDL were also observed and quantified by using an atomic force microscope (AFM). The impedance measurement of PDL-MUA-SAMs modified MEA only increased slightly to an average of 524.6 +/- 55.8 kOmega from 352.9 +/- 34.4 kOmega of bare gold microelectrode (p < 0.05, N = 20). In addition, the time-course changes of total impedance resulting from cell sealing resistance and gap reactance were recorded for 7 days for inferring the growth of cell lines on the electrode contact of modified MEA. The experiment of 3T3 fibroblasts, PC12 cells, primary glial cells, and primary cortical neurons cultured on the modified MEAs displayed a good adhesion rate. These biocompatibility assays demonstrated that the neuronal cells are able to grow in a proximity to PDL-MUA-SAMs modified microelectrodes of the MEAs for effective electrophysiological stimulation/sensing schemes and for future implantation purposes.

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“…22 As shown in Fig. 4c, the binding energy at 134.1 eV belongs to P 2p of DMPC-SPIONs, 23,24 the N 1s spectra of DMPC-SPIONs (Fig. 4d) can be curve tted into three peaks by multiple Gaussians with an equal full width at halfmaximum (FWHM) using a Shirley-type background, and the binding energies at about 400, 401.34, and 403 eV are attributable to the N-H, C-N-C, and N + -(CH 3 ) 3 groups of DMPC-SPIONs.…”

Section: Characterization Of the Nanoparticlesmentioning

confidence: 95%