Enhanced cellular uptake of iron oxide nanoparticles modified with 1,2-dimyristoyl-sn-glycero-3-phosphocholine

Su, Lichao; Zhang, Baolin; Huang, Yeu‐Shiang; Fan, Zhihai; Zhao, Ying‐Zheng

doi:10.1039/c7ra06844a

Cited by 13 publications

(10 citation statements)

References 35 publications

(38 reference statements)

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“…ICP-OES demonstrated that an average of 12.8 ± 3.6 pg Fe was internalised into each cell (with 12.8 pg Fe equating to 16,487 nanoparticles) and subsequent Prussian blue staining confirmed this internalisation and identified the nanoparticles propensity to accumulate around the nuclear membrane. This level of iron uptake into cells is comparable to the results reported from similar protocols published previously [35][36][37]. Next, intracellular hyperthermia, extracellular hyperthermia, and intracellular and extracellular hyperthermia were compared to define differences in viability through Annexin V/7-AAD staining.…”

Section: Discussionsupporting

confidence: 83%

Comparing the Effects of Intracellular and Extracellular Magnetic Hyperthermia on the Viability of BxPC-3 Cells

et al. 2020

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Magnetic hyperthermia involves the use of iron oxide nanoparticles to generate heat in tumours following stimulation with alternating magnetic fields. In recent times, this treatment has undergone numerous clinical trials in various solid malignancies and subsequently achieved clinical approval to treat glioblastoma and prostate cancer in 2011 and 2018, respectively. However, despite recent clinical advances, many questions remain with regard to the underlying mechanisms involved in this therapy. One such query is whether intracellular or extracellular nanoparticles are necessary for treatment efficacy. Herein, we compare the effects of intracellular and extracellular magnetic hyperthermia in BxPC-3 cells to determine the differences in efficacy between both. Extracellular magnetic hyperthermia at temperatures between 40–42.5 °C could induce significant levels of necrosis in these cells, whereas intracellular magnetic hyperthermia resulted in no change in viability. This led to a discussion on the overall relevance of intracellular nanoparticles to the efficacy of magnetic hyperthermia therapy.

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Section: Discussionsupporting

confidence: 83%

Comparing the Effects of Intracellular and Extracellular Magnetic Hyperthermia on the Viability of BxPC-3 Cells

et al. 2020

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“…In our previous work, we tried to modify the surface of nanoparticles with DMPC (1,2-dimyristoyl- sn -glycero-3-phos-phocholine) and GSH (glutathione), but severe endocytosis of the nanoparticles occurred after their injection into the substantia nigra. , In this study, we examine CS surface modification to achieve extracellular distribution of nanoparticles, because CS is commonly found on the cell membrane. There are many CS receptors in cell membranes, such as CD-44, TLR4 (toll-like receptor 4), and intercellular bonding molecules, which would attach CS to the cell membrane, and CS becomes a component of the neural network system .…”

Section: Resultsmentioning

confidence: 99%

“…The raw materials used and the synthesis of PEI-PEG-SPIONs (PEG: poly(ethylene) glycol, PEI: polyethylene imine) are as previously reported. , 0.3 g of PEI was dissolved in 15 g of PEG at 80 °C with vigorous magnetic stirring in a three-neck round-bottom flask for 10 min, and then 0.7 g of Fe(acac) 3 was added to the flask with stirring for another 10 min. This red solution was heated to 260 °C at a rate of 7.2 °C/min under a flow of argon and held at that temperature for 1 h. The reactants were cooled to 60 °C by removing the heat source and then mixed with 60 mL of toluene; after ultrasonic stirring, the particles were precipitated by the adsorption of magnet and washed twice with acetone to remove the excess organics.…”

Section: Methodsmentioning

confidence: 99%

“…All animal procedures were carried out in accordance with the Institutional Animal Care and Use Committee. Five μL of 1 mg/mL PEG-PEI-SPIONs or CS-SPIONs in 0.01 M PBS and 5 μL of 0.01 M PBS (control) were injected into the substantia nigra of rat brains according to the procedures reported in our previous work. ,, The detailed operation and sampling can be found in the Supporting Information.…”

Section: Methodsmentioning

confidence: 99%

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Surface Modification with Chondroitin Sulfate Targets Nanoparticles to the Neuronal Cell Membrane in the Substantia Nigra

Nie

Zhang

Pan

et al. 2019

ACS Chem. Neurosci.

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Localizing nanoparticles on or near cell membranes in vivo remains a big challenge. We present a cell membrane targeting complex based on chondroitin sulfate (CS)-conjugated superparamagnetic iron oxide nanoparticles (CS-SPIONs). After SPIONs were injected into the substantia nigra of rats, the subcellular distributions of SPIONs with and without CS modification have been evaluated by transmission electron microscopy (TEM) analysis. CS-SPIONs exhibited low toxicity and low endocytosis and were highly distributed in the extracellular spaces nearing neuronal cell bodies and synapses. This can be attributed to the nature of CS, one of the main components of perineuronal nets with the tendency to surround neuronal cell bodies, dendrites, and synapses. It is expected that CS-SPIONs have a great potential for therapies requiring targeting of or approach to cell membranes.

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“…Polymer‐based surface coatings yield effective steric stabilization of the iron surfaces and prevent aggregation of particles [ 20b ] and are the most typical approach. Passivating SPIOs with PVP, [ 155 ] polyglycerol adipate, [ 156 ] or polyethyleneimine (PEI) [ 157 ] via either in situ or ex situ synthetic processes are commonly reported. In particular, the primary amine groups present in PEI molecule enable their conjugation with tumor‐targeting molecules or other functional molecules.…”

Section: Metal‐based Mri Contrast Agentsmentioning

confidence: 99%

Metal‐Based Nanoparticle Magnetic Resonance Imaging Contrast Agents: Classifications, Issues, and Countermeasures toward their Clinical Translation

Antwi-Baah

Wang

Chen

et al. 2022

Adv Materials Inter

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Over the years, several imaging techniques have been developed to improve alreadyexisting ones, thus, overcoming the obstacles that previous ones failed to overcome. Recently, much energy has been put into developing tools that complement the ability of the current imaging techniques. Imaging probe or contrast agent is one of the well-known tools in this category, which enhance the conspicuousness of images. Researchers have taken the opportunity to work tirelessly on developing imaging probes, which have gone a long way in attaining biological and functional image details of a biological system when used alongside existing imaging techniques. With the advantage of displaying a more detailed view for analyzing biological information and diagnosing diseases, imaging probes are undoubtedly a significant research field in biological and medical sciences.Molecular imaging, a term describing the visualization of specific molecules and molecular transformations that occur during disease activity in a living system using radiological and nuclear medicine, [2] has recently received enormous attention. A tissue or organ is diseased when it depicts characteristics that deviate from its normal functionality or organization. The diseased condition is stimulated by many biochemical processes involving internal and external stimuli from within the cell. Molecular imaging serves as a diagnostic tool for detecting abnormal disease molecules early because it can examine the biochemical adjustments as a normal tissue mutates into a diseased one. Representative imaging techniques include magnetic particle imaging (MPI), [3] positron emission tomography (PET), single-photon emission computed tomography (SPECT), optical imaging, magnetic resonance imaging (MRI), computed tomography (CT) and, ultrasound. [4] These imaging techniques are noninvasive diagnostic platforms that bridge the gap between molecular biology and molecular medicine. They allow examining a living organism to verify effects on multiple organ systems in vivo and help diagnose diseases at preclinical stages. These imaging techniques employ tracers or contrast agents to improve sensitivity. MPI is a tracer imaging modality that instantaneously quantifies the concentration and Metal-based nanoparticles, especially gadolinium, manganese, and iron, have gained ground in the research of magnetic resonance imaging (MRI) contrast agents. Over the years, contrast agents based on these paramagnetic and superparamagnetic nanomaterials have merited keen attention in the biomedical field due to their desired properties such as sizeable magnetic susceptibility, tunable size, easy surface functionalization, low toxicity, etc. Gadolinium-based chelates are the traditional MRI contrast agents in the clinic but require improved relaxivities and pharmacokinetics. Nanoparticles possess a larger surface area, demonstrate a longer retention time in the body, and allow the conjugation of several functional molecules to enhance tumor targeting, making them more advantageous. The pursuit o...

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Enhanced cellular uptake of iron oxide nanoparticles modified with 1,2-dimyristoyl-sn-glycero-3-phosphocholine

Abstract: DMPC greatly enhanced the cellular uptake of SPIONs, resulting in remarkable amounts of accumulated nanoparticles in PC-12 cells.

Cited by 13 publications

References 35 publications

Comparing the Effects of Intracellular and Extracellular Magnetic Hyperthermia on the Viability of BxPC-3 Cells

Comparing the Effects of Intracellular and Extracellular Magnetic Hyperthermia on the Viability of BxPC-3 Cells

Surface Modification with Chondroitin Sulfate Targets Nanoparticles to the Neuronal Cell Membrane in the Substantia Nigra

Metal‐Based Nanoparticle Magnetic Resonance Imaging Contrast Agents: Classifications, Issues, and Countermeasures toward their Clinical Translation

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