Characterization of surface modification on microelectrode arrays for in vitro cell culture

Lin, San‐Yih; Chen, Jia Jin J.; Liao, Jiunn Der; Tzeng, Shun Fen

doi:10.1007/s10544-007-9114-y

Cited by 11 publications

(19 citation statements)

References 35 publications

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“…Previously, it was shown that determination of cell damage, cell growth, and cell morphology, could serve as preliminary indices for evaluating the non-cytotoxicity of medical devices [25, 35, 36]. In our study, characterization of cells cultured in the degraded 3D matrix with a perfusion system on MEA indicated cell survival and growth in the in-vitro 3D matrix.…”

Section: Discussionmentioning

confidence: 67%

“…The impedance measurement is essential for validating successful fabrication and chemical modification of self-designed MEAs when AFM or SEM methods are generally unsuitable for all electrode samples [25, 26, 36, 39]. In accordance with our previous experience, it is commonly suggested that the total impedance exceeding 800 kΩ after surface modification should be considered a modification failure [25].…”

Section: Discussionmentioning

confidence: 99%

“…The gold-based planar MEAs with a substrate of biocompatible polyimide (PI, Durimide 7320SE, Arch Chemicals) were fabricated on a cleaning glass substrate (4.5×4.5 cm 2 ) by using standard MEMS lithography technology as described previously [25]. PI was chosen as a substrate based on its insulation properties for the layout of stimulation/sensing microelectrodes and its biocompatibility for in-vitro cell culture.…”

Section: Methodsmentioning

confidence: 99%

“…Subsequently, photosensitive PI was respun to create the protective layer. After fabrication and hard bake (baked at 100°C for 1 hr), planar MEAs were then surface modified by 11-mercaptoundecanoic acid (MUA, COOH-(CH 2 ) 10 -SH, Aldrich) SAMs and PDL in accordance with previous experimental protocols [25]. Afterwards, 3D matrix was synthesized by pouring 500 μl hydrogel-precursor solution into the planar surface-modified MEA and exposing to visible light for 3 min.…”

Section: Methodsmentioning

confidence: 99%

“…Among the various electrical properties of cells, impedance measurement is an electrochemical method usually expressed as the capacitive and resistive components that is used to monitor changes in biological environments [25-27]. Among various biological applications, impedance measurement has been utilized to estimate the effects of electrode materials on neural prosthetic devices in vitro [10, 28], to study changes in impedance of stratified cell layers on MEAs [1, 16, 25, 26], and to assess variations in tissue resistances in vivo [27, 29]. In addition, impedance measurements have been used in cell-based sensing applications such as alteration of cell membranes or morphological changes of adherent cell layers [1, 11, 16, 25].…”

Section: Introductionmentioning

confidence: 99%

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On-line observation of cell growth in a three-dimensional matrix on surface-modified microelectrode arrays

2009

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Despite many successful applications of microelectrode arrays (MEAs), typical two-dimensional in-vitro cultures do not project the full scale of the cell growth environment in the three-dimensional (3D) in-vivo setting. This study aims to on-line monitor in-vitro cell growth in a 3D matrix on the surface-modified MEAs with a dynamic perfusion culture system. A 3D matrix consisting of poly(ethylene glycol) hydrogel supplemented with poly-D-lysine was subsequently synthesized in-situ on the self-assembled monolayers modified MEAs. FTIR spectrum analysis revealed a peak at 2100 cm−1 due to the degradation of the structure of the 3D matrix. After 2 wks, microscopic examination revealed that the non-degraded area was around 1500 μm2 and provided enough space for cell growth. Fluorescence microscopy revealed that the degraded 3D matrix was non-cytotoxic allowing the growth of NIH3T3 fibroblasts and cortical neurons in vitro. Time-course changes of total impedance including resistance and reactance were recorded for 8 days to evaluate the cell growth in the 3D matrix on the MEA. A consistent trend reflecting changes of reactance and total impedance was observed. These in-vitro assays demonstrate that our 3D matrix can construct a biomimetic system for cell growth and analysis of cell surface interactions.

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Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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On-line observation of cell growth in a three-dimensional matrix on surface-modified microelectrode arrays

2009

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Impedimetric monitoring of IGF-1 protection of in vitro cortical neurons under ischemic conditions

Huang

Lin

Liang

et al. 2012

Biomed Microdevices

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Microelectrode arrays (MEAs) incorporated with the electric cell substrate impedance sensing (ECIS) technique provide a method for acquiring cellular electrophysiological information, which is useful for the time-course monitoring of cellular outgrowth and damage. This research utilizes the ECIS technique for monitoring the time-course impedimetric changes in normal and insulin-like growth factor 1 (IGF-1)-protected cortical neurons under the ischemic insult of oxygen glucose deprivation (OGD) created in a microperfusion environment. The neuronal apoptosis is reflected by the relatively low cell viability (28 ± 11.5 %) after 30-min OGD followed by 24 h of re-oxygenation. Also the hyperpolarization phase of mitochondrial membrane potential (MMP) occurs during 2 h of the re-oxygenation period. In contrast, cortical neurons treated with 50 and 100 ng/mL IGF-1 show higher survival rates of 45 ± 5.2 % and 49 ± 9.2 %, respectively, and no occurrence of the hyperpolarization of MMP during the re-oxygenation period. The ECIS results demonstrate that the measured impedance of cortical neurons decreased from 826 ± 86 kΩ to 224 ± 32 kΩ due to cell detachment under the insult of OGD. The measured impedance of IGF-1-protected cortical neurons slowly decreased to about 50 % of the original value (560 ± 45 kΩ for 50 ng/mL and 593 ± 44 kΩ for 100 ng/mL) compared to saline control of 232 ± 37 kΩ, which indicates improved cell adhesion under OGD conditions. The time-course impedimetric results show that the proposed ECIS-based MEAs platform incorporated with a microperfusion environment can be used for the real-time monitoring of cortical neurons under in vitro OGD and the IGF-1 protective effect against OGD-induced ischemic neuronal death.

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Simultaneous recording of electrical activity and the underlying ionic currents in NG108-15 cells cultured on gold substrate

Acosta-García

Morales-Reyes

Jiménez-Anguiano

et al. 2018

Heliyon

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This paper shows the simultaneous recording of electrical activity and the underlying ionic currents by using a gold substrate to culture NG108-15 cells. Cells grown on two different substrates (plastic Petri dishes and gold substrates) were characterized quantitatively through scanning electron microscopy (SEM) as well as qualitatively by optical and atomic force microscopy (AFM). No significant differences were observed between the surface area of cells cultured on gold substrates and Petri dishes, as indicated by measurements performed on SEM images. We also evaluated the electrophysiological compatibility of the cells through standard patch-clamp experiments by analyzing features such as the resting potential, membrane resistance, ionic currents, etc. Cells grown on both substrates showed no significant differences in their dependency on voltage, as well as in the magnitude of the Na+ and K+ current density; however, cells cultured on the gold substrate showed a lower membrane capacitance when compared to those grown on Petri dishes. By using two separate patch-clamp amplifiers, we were able to record the membrane current with the conventional patch-clamp technique and through the gold substrate simultaneously. Furthermore, the proposed technique allowed us to obtain simultaneous recordings of the electrical activity (such as action potentials firing) and the underlying membrane ionic currents. The excellent conductivity of gold makes it possible to overcome important difficulties found in conventional electrophysiological experiments such as those presented by the resistance of the electrolytic bath solution. We conclude that the technique here presented constitutes a solution to the problem of the simultaneous recording of electrical activity and the underlying ionic currents, which for decades, had been solved only partially.

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Characterization of surface modification on microelectrode arrays for in vitro cell culture

Cited by 11 publications

References 35 publications

On-line observation of cell growth in a three-dimensional matrix on surface-modified microelectrode arrays

On-line observation of cell growth in a three-dimensional matrix on surface-modified microelectrode arrays

Impedimetric monitoring of IGF-1 protection of in vitro cortical neurons under ischemic conditions

Simultaneous recording of electrical activity and the underlying ionic currents in NG108-15 cells cultured on gold substrate

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