Labeling the nucleocapsid of enveloped baculovirus with quantum dots for single-virus tracking

Wen, Li; Lin, Yi; Zheng, Zhenhua; Zhang, Zhiling; Zhang, Lijuan; Wang, Liying; Wang, Hanzhong; Pang, Dai‐Wen

doi:10.1016/j.biomaterials.2013.11.069

Cited by 48 publications

(49 citation statements)

References 31 publications

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“…1a) was prepared as in our previous report [11] (see also the “Methods”). QDs-RBV can be easily obtained by incubating RBV with SA-QDs, probably because the internal capsid would be partially exposed with the loss of some loose envelope during virus purification with ultracentrifugation [18, 19].…”

Section: Resultsmentioning

confidence: 99%

“…QDs-RBV can be easily obtained by incubating RBV with SA-QDs, probably because the internal capsid would be partially exposed with the loss of some loose envelope during virus purification with ultracentrifugation [18, 19]. Moreover, the infectivity of viruses was maintained during the labeling and subsequent purification processes [11]. As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…QDs-RBVs were prepared as in our previous report [11]. Briefly, RBVs with internal biotinylated capsids were produced by infecting Sf9 cells with recombinant bacmids.…”

Section: Methodsmentioning

confidence: 99%

“…As the prerequisite for infection, the movements of viruses from cell surface to nucleus, the so-called retrograde transportation, has aroused extensive attention in past decades [3–5]. Recently, owing to the superior brightness and stability of quantum dots (QDs) [6], QD-based single virus tracking (SVT) has become a powerful tool for investigating infection dynamics of viruses at the single-virus level by providing in situ and real-time evidences [7–11]. To date, by employing SVT based on QD-labeling of virus external envelope, information on the early stages of virus retrograde transportation has been provided [6–9].…”

Section: Introductionmentioning

confidence: 99%

“…We have proposed a mild approach for preparing recombinant baculovirus (RBV) with QDs labeled capsid (QDs-RBV) [11]. By infecting host Spodoptera frugiperda 9 (Sf9) cells with recombinant bacmids, RBV with biotinylated capsid was obtained and subsequently labeled with streptavidin conjugated QDs.…”

Section: Introductionmentioning

confidence: 99%

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Tracking single baculovirus retrograde transportation in host cell via quantum dot-labeling of virus internal component

et al. 2017

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BackgroundQuantum dot (QD)-based single virus tracking has become a powerful tool for dissecting virus infection mechanism. However, only virus behaviors at the early stage of retrograde trafficking have been dynamically tracked so far. Monitoring of comprehensive virus retrograde transportation remains a challenge.ResultsBased on the superior fluorescence properties of QDs and their labeling of virus internal component, the dynamic interactions between baculoviruses and all key transportation-related cellular structures, including vesicles, acidic endosomes, actins, nuclear pores and nuclei, were visualized at the single-virus level. Detailed scenarios and dynamic information were provided for these critical interaction processes.ConclusionsA comprehensive model of baculovirus retrograde trafficking involving virus endocytosis, fusion with acidic endosome, translocation to nuclear periphery, internalization into nucleus, and arriving at the destination in nucleus was proposed. Thus the whole retrograde transportation of baculovirus in live host cells was elucidated at the single-virus level for the first time.Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-017-0270-9) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…QDs-RBVs were prepared as in our previous report [11]. Briefly, RBVs with internal biotinylated capsids were produced by infecting Sf9 cells with recombinant bacmids.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tracking single baculovirus retrograde transportation in host cell via quantum dot-labeling of virus internal component

et al. 2017

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Transformation of Cell‐Derived Microparticles into Quantum‐Dot‐Labeled Nanovectors for Antitumor siRNA Delivery

et al. 2014

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Cell‐derived microparticles (MPs) have been recently recognized as critical intercellular information conveyors. However, further understanding of their biological behavior and potential application has been hampered by the limitations of current labeling techniques. Herein, a universal donor‐cell‐assisted membrane biotinylation strategy was proposed for labeling MPs by skillfully utilizing the natural membrane phospholipid exchange of their donor cells. This innovative strategy conveniently led to specific, efficient, reproducible, and biocompatible quantum dot (QD) labeling of MPs, thereby reliably conferring valuable traceability on MPs. By further loading with small interference RNA, QD‐labeled MPs that had inherent cell‐targeting and biomolecule‐conveying ability were successfully employed for combined bioimaging and tumor‐targeted therapy. This study provides the first reliable and biofriendly strategy for transforming biogenic MPs into functionalized nanovectors.

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Transformation of Cell‐Derived Microparticles into Quantum‐Dot‐Labeled Nanovectors for Antitumor siRNA Delivery

et al. 2014

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View full text Add to dashboard Cite

Cell-derived microparticles (MPs) have been recently recognized as critical intercellular information conveyors. However, further understanding of their biological behavior and potential application has been hampered by the limitations of current labeling techniques. Herein, a universal donor-cell-assisted membrane biotinylation strategy was proposed for labeling MPs by skillfully utilizing the natural membrane phospholipid exchange of their donor cells. This innovative strategy conveniently led to specific, efficient, reproducible, and biocompatible quantum dot (QD) labeling of MPs, thereby reliably conferring valuable traceability on MPs. By further loading with small interference RNA, QD-labeled MPs that had inherent cell-targeting and biomolecule-conveying ability were successfully employed for combined bioimaging and tumor-targeted therapy. This study provides the first reliable and biofriendly strategy for transforming biogenic MPs into functionalized nanovectors.

show abstract

Labeling the nucleocapsid of enveloped baculovirus with quantum dots for single-virus tracking

Cited by 48 publications

References 31 publications

Tracking single baculovirus retrograde transportation in host cell via quantum dot-labeling of virus internal component

Tracking single baculovirus retrograde transportation in host cell via quantum dot-labeling of virus internal component

Transformation of Cell‐Derived Microparticles into Quantum‐Dot‐Labeled Nanovectors for Antitumor siRNA Delivery

Transformation of Cell‐Derived Microparticles into Quantum‐Dot‐Labeled Nanovectors for Antitumor siRNA Delivery

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