Transformation of Cell‐Derived Microparticles into Quantum‐Dot‐Labeled Nanovectors for Antitumor siRNA Delivery

Abstract

Cell‐derived microparticles (MPs) have been recently recognized as critical intercellular information conveyors. However, further understanding of their biological behavior and potential application has been hampered by the limitations of current labeling techniques. Herein, a universal donor‐cell‐assisted membrane biotinylation strategy was proposed for labeling MPs by skillfully utilizing the natural membrane phospholipid exchange of their donor cells. This innovative strategy conveniently led to specific, efficient, reproducible, and biocompatible quantum dot (QD) labeling of MPs, thereby reliably conferring valuable traceability on MPs. By further loading with small interference RNA, QD‐labeled MPs that had inherent cell‐targeting and biomolecule‐conveying ability were successfully employed for combined bioimaging and tumor‐targeted therapy. This study provides the first reliable and biofriendly strategy for transforming biogenic MPs into functionalized nanovectors.

Membrane microvesicles as mediators for melanoma-fibroblasts communication: Roles of the VCAM-1/VLA-4 axis and the ERK1/2 signal pathway

“…The hydrodynamic diameter and polydispersity index (PDI) of the suspended microvesicles were determined with a Nano-ZS ZEN 3600 instrument (Malvern Instruments, UK), as previously reported [12].…”

Characterization of microparticles in patients with venous malformations of the head and neck

“…The obtained MP were quantified by flow cytometry using BD FACSAria III flow cytometer (BD Biosciences, USA) as similar to our previous reports (Chen et al , ; Ren et al , ,b). Briefly, when a sample is loaded into FACSAria, the count of ‘events’ could be detected and obtained through flow cytometer.…”

“…The supernatant was collected and centrifuged at 1550 g for another 20 min to obtain platelet‐free plasma. The supernatant were collected again and then centrifuged at 50 000 g at 4°C for 1 h to concentrate MP (Witek et al , ; Baran et al , ; Chen et al , ; Ren et al , ). After re‐suspension with 150 μ l Ca 2+ /Mg 2+ ‐free highly purified phosphate‐buffered saline (PBS; Beyotime, China), the obtained MP were divided into three parts: 50 μ l for flow cytometry analysis, 50–70 μ l for RNA extraction, and the rest for direct storage at −80°C until subsequent use.…”

Lymphocyte‑derived microparticles stimulate osteoclastogenesis by inducing RANKL in fibroblasts of odontogenic keratocysts

“…The hydrodynamic diameters of CFMPs were determined by a Nano-ZS ZEN 3600 (Malvern Instruments, Inc., Westborough, MA, USA), which was equipped with a He-Ne laser (633 nm), as previously described (15).…”