Label free structure-switching fluorescence polarization detection of chloramphenicol with truncated aptamer

Ma, Peisheng; Guo, Hualin; Duan, Nuo; Ma, Xiaoyuan; Yue, Lin; Gu, Qianhui; Wang, Zhouping

doi:10.1016/j.talanta.2021.122349

Cited by 41 publications

(17 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The predicted secondary structures of the candidate aptamers had abundant secondary structures, which were mainly based on the typical stable loop-stem and bulge structures. The results indicate that the stem-loop structures 30,31 could play a major role in their binding to target serum. The predicted secondary structures of aptamers Ap-LC-2, Ap-LC-3, Ap-LC-6 and Ap-LC-19 with minimum free energies (DG) of À20.20 and À22.80 kcal mol À1 , respectively, are shown in Fig.…”

Section: High-throughput Sequencing and Analysismentioning

confidence: 91%

Identification of a novel DNA aptamer that selectively targets lung cancer serum

Zhao

Huang

et al. 2021

RSC Adv.

View full text Add to dashboard Cite

show abstract

Section: High-throughput Sequencing and Analysismentioning

confidence: 91%

Identification of a novel DNA aptamer that selectively targets lung cancer serum

Zhao

Huang

et al. 2021

RSC Adv.

View full text Add to dashboard Cite

show abstract

“…The biosensor used in this study only consisted of one aptamer and two split probes. The preparation was easy and the requirements for storage conditions were lower compared with ternary Ag2CrO4/g-C3N4/GO nanocomposite 15 , fluorochrome 16 , labeled oligonucleotide 17 , bimetallic organic framework 18 , GO-QDs nanocomposites and enzyme-linked aptamers 19 , and gold nanoparticles 20 in previous studies. The simple reaction system of our assay also resulted in a lower cost, about 0.16 dollars per reaction (the cost included oligonucleotide synthesis, reagents, and consumables).…”

Section: Detection Limit and Linear Detection Range Of The Biosensormentioning

confidence: 96%

“…Thus, they show good potential in the detection of pathogens 13 , metal ions 14 and antibiotics including CAP 10 . As with antibodies, aptamers targeting CAP always combine with other indicators or catalytic labels such as ternary Ag2CrO4/g-C3N4/GO nanocomposite 15 , fluorochrome 16 , labeled oligonucleotide 17 , bimetallic organic framework 18 , GO-QDs nanocomposites 19 , and gold nanoparticles 20 . However, they are still insufficiently sensitive, complex in preparation or dependent on specifical equipment.…”

Section: Introductionmentioning

confidence: 99%

A visual on-site biosensor for low-cost detection of chloramphenicol based on aptamer and split DNAzyme

Chen

et al. 2022

ANAL. SCI.

View full text Add to dashboard Cite

Chloramphenicol (CAP) is a kind of broad-spectrum antibiotics, which has been forbidden in food by most countries because of its side effects. In this study, a simple and low-cost biosensor for CAP detection in food was developed. The biosensor only consisted of an aptamer specific to CAP and a pair of split probes that could self-assemble as DNAzyme. The detection result could be identified by the naked eye and the visual limit was 10 nM CAP. The absorbance of final reaction products at 417 nm had a linear relationship with the logarithm of the CAP concentration ranged from 10 to 200 nM, and the limit of detection was 87.3 pM. The visual analysis by imageJ also showed a linear detection range between 25 and 200 nM. The whole detection could be finished in about 1.5 hours with about 0.16 dollars per reaction. We believe that the biosensor shows great potential in the rapid and sensitive detection of CAP in food.

show abstract

“…Aptamer-based fluorescence sensing detection is a relatively common analysis method. The fluorophore is combined with the aptamer in a labeled or non-labeled manner, and the analyte concentration and other information are reflected by the interaction of the excitation light and the identification element [29,30]. Furthermore, fluorescence intensity, decay rate, spectral properties, and fluorescence anisotropy can be used alone or in combination as signal detection means.…”

Section: Introductionmentioning

confidence: 99%

Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices

Hong

Pan

Xie

et al. 2021

Foods

View full text Add to dashboard Cite

Food allergies have seriously affected the life quality of some people and even endangered their lives. At present, there is still no effective cure for food allergies. Avoiding the intake of allergenic food is still the most effective way to prevent allergic diseases. Therefore, it is necessary to develop rapid, accurate, sensitive, and reliable analysis methods to detect food allergens from different sources. Aptamers are oligonucleotide sequences that can bind to a variety of targets with high specificity and selectivity, and they are often combined with different transduction technologies, thereby constructing various types of aptamer sensors. In recent years, with the development of technology and the application of new materials, the sensitivity, portability, and cost of fluorescence sensing technology have been greatly improved. Therefore, aptamer-based fluorescence sensing technology has been widely developed and applied in the specific recognition of food allergens. In this paper, the classification of major allergens and their characteristics in animal and plant foods were comprehensively reviewed, and the preparation principles and practical applications of aptamer-based fluorescence biosensors are summarized. In addition, we hope that this article can provide some strategies for the rapid and sensitive detection of allergens in food matrices.

show abstract

Label free structure-switching fluorescence polarization detection of chloramphenicol with truncated aptamer

Cited by 41 publications

References 52 publications

Identification of a novel DNA aptamer that selectively targets lung cancer serum

Identification of a novel DNA aptamer that selectively targets lung cancer serum

A visual on-site biosensor for low-cost detection of chloramphenicol based on aptamer and split DNAzyme

Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices

Contact Info

Product

Resources

About