Abstract:Chloramphenicol (CAP) is a kind of broad-spectrum antibiotics, which has been forbidden in food by most countries because of its side effects. In this study, a simple and low-cost biosensor for CAP detection in food was developed. The biosensor only consisted of an aptamer specific to CAP and a pair of split probes that could self-assemble as DNAzyme. The detection result could be identified by the naked eye and the visual limit was 10 nM CAP. The absorbance of final reaction products at 417 nm had a linear re… Show more
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