Label-Free Quantitative Proteomic Analysis of Chitosan Oligosaccharide-Treated Rice Infected with Southern Rice Black-Streaked Dwarf Virus

Abstract

Southern rice black-streaked dwarf virus (SRBSDV) has spread from thesouth of China to the north of Vietnam in the past few years and severelyinfluenced rice production. Its long incubation period and early symptoms are not evident; thus, controlling it is difficult. Chitosan oligosaccharide (COS) is a green plant immunomodulator. Early studies showed that preventing and controlling SRBSDV have a certain effect and reduce disease infection rate, but its underlying controlling and preventing mechanism is unclear. In this study, label-free proteomics was used to analyze differentially expressed proteins in rice after COS treatment. The results showed that COS can up-regulate the plant defense-related proteins and down-regulate the protein expression levels of SRBSDV. Meanwhile, quantitative real-time PCR test results showed that COS can improve defense gene expression in rice. Moreover, COS can enhance the defense enzymatic activities of peroxidase, superoxide dismutase and catalase through mitogen-activated protein kinase signaling cascade pathway, and enhance the rice disease resistance.

Comparative proteomic analysis of maize (Zea mays L.) seedlings under rice black-streaked dwarf virus infection

“…In rice, cross-talk between the responses to H 2 O 2 treatment and to RBSDV infection suggested pivotal roles of H 2 O 2 in the responses to RBSDV infection [ 17 ]. Recently, a label-free quantitative proteomic analysis showed that chitosan oligosaccharide enhanced the resistance of rice to RBSDV infection by activating the mitogen-activated protein kinase signaling cascade pathway [ 18 ]. To investigate the responses of maize seedlings to RBSDV infection at the proteome level, comparative proteomic analysis was performed.…”

Design, synthesis, and insecticidal activity evaluation of novel 4‐(N, N‐diarylmethylamines)furan‐2(5H)‐one derivatives as potential acetylcholine receptor insecticides

“…The concentration of total protein was calculated according to the Bradford method . The protein was digested with trypsin using the reported methods, and the enzymatic hydrolysate was dried for 6 h in a vacuum drier, and the dry residue was used for LC–MS/MS analysis.…”

Synthesis and Insecticidal Activity of Mesoionic Pyrido[1,2-α]pyrimidinone Derivatives Containing a Neonicotinoid Moiety

“…Then, the concentration of total protein was determined using the Bradford method [ 49 ]. Before the LC-MS/MS analysis, protein was digested with trypsin using reported methods [ 50 ].…”