Comparative proteomic analysis of maize (Zea mays L.) seedlings under rice black-streaked dwarf virus infection

Yue, Runqing; Lu, Chengpeng; Han, Xiaohua; Guo, Songlin; Yan, Shufeng; Liu, Lu; Xiaolei, Fu; Chen, Nana; Guo, Xinhai; Chi, Haifeng; Tie, Shuanggui

doi:10.1186/s12870-018-1419-x

Cited by 19 publications

(15 citation statements)

References 41 publications

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“…In this work, one proline-related protein (XP_008454439.1) was up-regulated in JS, while its expression remained unchanged in GE. Our study also suggested that three cyanoamino acid relevant proteins (NP_001303611.1, XP_008452081.1, XP_016899729.1) were up-regulated in JS, which is in accordance with the findings of Yue et al [52] (Table 2).…”

Section: Amino Acids Metabolism Related Proteinssupporting

confidence: 92%

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

Song

Tang

Cai

et al. 2020

Preprint

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Background: Cantaloupe is susceptible to cold stress when it is stored at low temperatures, resulting in the loss of edible and commercial quality. To ascertain the molecular mechanisms of low temperatures resistance in cantaloupe, a cold-sensitive cultivar, Golden Empress-308 (GE) and a cold-tolerant cultivar, Jia Shi-310 (JS), were selected in parallel for iTRAQ quantitative proteomic analysis. Results: The two kinds of commercial cultivars were exposed to a temperature of 0.5℃ for 0, 12 and 24 days. We found that the cold-sensitive cultivar (GE) suffered more severe damage as the length of the cold treatment increased. Proteomic analysis of both cultivars indicated that the number of differentially expressed proteins (DEPs) changed remarkably during the chilly treatment. JS expressed cold-responsive proteins more rapidly and mobilized more groups of proteins than GE. Furthermore, metabolic analysis revealed that more amino acids were up-regulated in JS during the early phases of low temperatures stress. The DEPs we found were mainly related to carbohydrate and energy metabolism, structural proteins, reactive oxygen species scavenging, amino acids metabolism and signal transduction. The consequences of phenotype assays, metabolic analysis and q-PCR validation confirm the findings of the iTRAQ analysis. Conclusion: We found that the prompt response and mobilization of proteins in JS allowed it to maintain a higher level of cold tolerance than GE, and that the slower cold responses in GE may be a vital reason for the severe chilling injury commonly found in this cultivar. The candidate proteins we identified will form the basis of future studies and may improve our understanding of the mechanisms of cold tolerance in cantaloupe.

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Section: Amino Acids Metabolism Related Proteinssupporting

confidence: 92%

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

Song

Tang

Cai

et al. 2020

Preprint

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“…In the past few years, microarray and RNA-Seq data have shown that hundreds of genes are up-and down-regulated on the transcript level in compatible and incompatible plant-pathogen interactions [22,[35][36][37][38]. Nevertheless, although proteomics approaches have been used to investigate plant-pathogen interactions, high-throughput proteomic data remain insu cient [39][40][41][42][43]. In this study, we used the LC-MS/MS technique to characterize the proteomics of the interactions between maize and S. turcica.…”

Section: Discussionmentioning

confidence: 99%

Comparative proteomic analysis revealed maize (Zea mays L.) response to Setosphaeria turcica early infection

Yu-wei¹,

Gong²,

Zhou³

et al. 2020

Preprint

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Background Globally, maize (Zea mays L.) is an important crop. Maize production is severely affected by northern corn leaf blight (NCLB), a destructive foliar disease caused by Setosphaeria turcica. Interaction between maize and S. turcica has not been characterised at protein level. Results We used tandem mass tag labelling and liquid chromatography–tandem mass spectrometry to investigate the proteomes of maize leaves at 24 and 72 h post-inoculation (hpi) with S. turcica. In total, 4,740 proteins were quantified which encoded by 4,711 genes. Clustering analyses provided insight into the dynamic reprogramming of leaves proteomes by revealing the functions of different proteins during S. turcica infection. Screening and classification of differentially expressed proteins (DEPs) revealed that numerous defence-related proteins, including defence marker proteins, proteins related to the phenylpropanoid lignin biosynthesis, benzoxazinone biosynthesis and jasmonic acid signal pathway, participated in the defence responses of maize to S. turcica infection. Furthermore, the early induction of GST family proteins could contribute to the resistance of S. turcica. In addition, the protein–protein interaction network of DEPs suggested that some defence-related proteins, e.g. ZmGEB1, play key roles in defence responses against S. turcica infection. Conclusion This study provides insight into the complex defence responses triggered by S. turcica at the protein level, as well as a new basis for studying the interaction process between maize and S. turcica at the earlier stage of infection.

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“…In the GO enrichment analysis, proteins were classified by GO annotation into three categories: biological process, cellular compartment, and molecular function. For each category, a two-tailed Fisher's exact test was employed to compare the enrichment of the differentially expressed protein against all identified proteins, and corrected p-values < 0.05 were considered significant [67,68].…”

Section: Data Analysis and Interpretationmentioning

confidence: 99%

Equine Herpesvirus Type 1 Modulates Cytokine and Chemokine Profiles of Mononuclear Cells for Efficient Dissemination to Target Organs

Pavulraj

Kamel

Stephanowitz

et al. 2020

Viruses

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Equine herpesvirus type 1 (EHV-1) causes encephalomyelopathy and abortion, for which cell-associated viremia and subsequent virus transfer to and replication in endothelial cells (EC) are responsible and prerequisites. Viral and cellular molecules responsible for efficient cell-to-cell spread of EHV-1 between peripheral blood mononuclear cells (PBMC) and EC remain unclear. We have generated EHV-1 mutants lacking ORF1, ORF2, and ORF17 genes, either individually or in combination. Mutant viruses were analyzed for their replication properties in cultured equine dermal cells, PBMC infection efficiency, virus-induced changes in the PBMC proteome, and cytokine and chemokine expression profiles. ORF1, ORF2, and ORF17 are not essential for virus replication, but ORF17 deletion resulted in a significant reduction in plaque size. Deletion of ORF2 and ORF17 gene significantly reduced cell-to-cell virus transfer from virus-infected PBMC to EC. EHV-1 infection of PBMC resulted in upregulation of several pathways such as Ras signaling, oxidative phosphorylation, platelet activation and leukocyte transendothelial migration. In contrast, chemokine signaling, RNA degradation and apoptotic pathways were downregulated. Deletion of ORF1, ORF2 and ORF17 modulated chemokine signaling and MAPK pathways in infected PBMC, which may explain the impairment of virus spread between PBMC and EC. The proteomic results were further confirmed by chemokine assays, which showed that virus infection dramatically reduced the cytokine/chemokine release in infected PBMC. This study uncovers cellular proteins and pathways influenced by EHV-1 after PBMC infection and provide an important resource for EHV-1 pathogenesis. EHV-1-immunomodulatory genes could be potential targets for the development of live attenuated vaccines or therapeutics against virus infection.

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Comparative proteomic analysis of maize (Zea mays L.) seedlings under rice black-streaked dwarf virus infection

Cited by 19 publications

References 41 publications

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

Comparative proteomic analysis revealed maize (Zea mays L.) response to Setosphaeria turcica early infection

Equine Herpesvirus Type 1 Modulates Cytokine and Chemokine Profiles of Mononuclear Cells for Efficient Dissemination to Target Organs

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