Label Free Mid-IR Photothermal Imaging of Bird Brain With Quantum Cascade Laser

Mërtiri, Alket; Totachawattana, Atcha; Liu, Hui; Hong, Mineui; Gardner, Tim; Sander, Michelle Y.; Erramilli, Shyamsunder

doi:10.1364/cleo_at.2014.af1b.4

Cited by 6 publications

(6 citation statements)

References 4 publications

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“…42−44 In conference proceedings, Mertiri et al showed photothermal imaging of dried bird brain tissue with the use of a mid-IR pump, near-IR probe, and a silicon objective lens. 45 Li et al demonstrated photothermal imaging of 1.1 μm polystyrene beads at the single particle level in water, glycerin, and carbon disulfide. 46 In 2016, the Cheng group reported a high-performance QCL-based MIP microscope and demonstrated, for the first time, 3D bond-selective imaging of living cells and organisms at a spatial resolution of 0.6 μm.…”

Section: Historical Development Of Mid-infrared Photothermal Microscopymentioning

confidence: 99%

“…Technological progress has also been made in photothermal spectroscopy. Erramilli and Sander reported nonlinear photothermal spectroscopy by using a QCL pump beam and nonlinear Zharov splitting in 50-μm homogeneous liquid crystal layers. − In conference proceedings, Mërtiri et al showed photothermal imaging of dried bird brain tissue with the use of a mid-IR pump, near-IR probe, and a silicon objective lens . Li et al demonstrated photothermal imaging of 1.1 μm polystyrene beads at the single particle level in water, glycerin, and carbon disulfide …”

Section: Historical Development Of Mid-infrared Photothermal Microscopymentioning

confidence: 99%

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Mid-Infrared Photothermal Microscopy: Principle, Instrumentation, and Applications

et al. 2022

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Midinfrared photothermal (MIP) microscopy, also called optical photothermal infrared (O-PTIR) microscopy, is an emerging tool for bond-selective chemical imaging of living biological and material samples. In MIP microscopy, a visible probe beam detects the photothermal-based contrast induced by a vibrational absorption. With submicron spatial resolution, high spectral fidelity, and reduced water absorption background, MIP microscopy has overcome the limitations in infrared chemical imaging methods. In this review, we summarize the basic principle of MIP microscopy, the different origins of MIP contrasts, and recent technology development that pushed the resolution, speed, and sensitivity of MIP imaging to a new stage. We further emphasize its broad applications in life science and material characterization, and provide a perspective of future technical advances.

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Section: Historical Development Of Mid-infrared Photothermal Microscopymentioning

confidence: 99%

Section: Historical Development Of Mid-infrared Photothermal Microscopymentioning

confidence: 99%

Mid-Infrared Photothermal Microscopy: Principle, Instrumentation, and Applications

et al. 2022

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“…As a result, they obtained photothermal images of 1.1 μm polystyrene beads in three different solvents. With an increased signal to baseline ratio, Mertiri et al 31 performed photothermal imaging of dried tissue slices. Nevertheless, the spatial resolution and sensitivity were not sufficient for detection of submicrometer features within a cell.…”

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confidence: 99%

Mid-Infrared Photothermal Imaging of Active Pharmaceutical Ingredients at Submicrometer Spatial Resolution

Zhang

Slipchenko

et al. 2017

Anal. Chem.

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Chemical imaging with sufficient spatial resolution to resolve microparticles in tablets is essential to ensure high quality and efficacy in controlled release. The existing modalities have the following disadvantages: they are time-consuming or have poor spatial resolution or low chemical specificity. Here, we demonstrate an epi-detected mid-infrared photothermal (epi-MIP) microscope at a spatial resolution of 0.65 μm. Providing identical spectral profiles as conventional infrared spectroscopy, our epi-MIP microscope enabled mapping of both active pharmaceutical ingredients and excipients of a drug tablet.

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“…Erramilli and colleagues demonstrated photothermal spectroscopy between 1860 and 1980 cm –1 of a liquid crystal in different phases . Sander and co-workers imaged bird brain tissue slices and characterized cancerous and healthy mouse brain tissue using the photothermal contrast for amide I band. , Zhang et al improved the spatial resolution to 0.61 μm and demonstrated imaging of live cells and Caenorhabditis elegans in transmission mode . Li et al illustrated the mapping of pharmaceutical ingredients in the fingerprint region with an epi-detected configuration .…”

Section: Introductionmentioning

confidence: 99%

“…12 Sander and co-workers imaged bird brain tissue slices and characterized cancerous and healthy mouse brain tissue using the photothermal contrast for amide I band. 13,14 Zhang et al improved the spatial resolution to 0.61 μm and demonstrated imaging of live cells and Caenorhabditis elegans in transmission mode. 15 Li et al illustrated the mapping of pharmaceutical ingredients in the fingerprint region with an epi-detected configuration.…”

Section: ■ Introductionmentioning

confidence: 99%

Bond-Selective Imaging of Cells by Mid-Infrared Photothermal Microscopy in High Wavenumber Region

Bai

Zhang

et al. 2017

J. Phys. Chem. B

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Using a visible beam to probe the thermal effect induced by infrared absorption, mid-infrared photothermal (MIP) microscopy allows bond-selective chemical imaging at submicron spatial resolution. Current MIP microscopes cannot reach the high wavenumber region due to the limited tunability of the existing quantum cascade laser source. We extend the spectral range of MIP microscopy by difference frequency generation (DFG) from two chirped femtosecond pulses. Flexible wavelength tuning in both C-D and C-H regions was achieved with mid-infrared power up to 22.1 mW and spectral width of 29.3 cm. Distribution of fatty acid in live human lung cancer cells was revealed by MIP imaging of the C-D bond at 2192 cm.

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Label Free Mid-IR Photothermal Imaging of Bird Brain With Quantum Cascade Laser

Cited by 6 publications

References 4 publications

Mid-Infrared Photothermal Microscopy: Principle, Instrumentation, and Applications

Mid-Infrared Photothermal Microscopy: Principle, Instrumentation, and Applications

Mid-Infrared Photothermal Imaging of Active Pharmaceutical Ingredients at Submicrometer Spatial Resolution

Bond-Selective Imaging of Cells by Mid-Infrared Photothermal Microscopy in High Wavenumber Region

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