Bond-Selective Imaging of Cells by Mid-Infrared Photothermal Microscopy in High Wavenumber Region

Bai, Yeran; Zhang, De‐Long; Li, Chen; Liu, Cheng; Cheng, Ji‐Xin

doi:10.1021/acs.jpcb.7b09570

Cited by 54 publications

(58 citation statements)

References 48 publications

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“…The concept of dynamic photothermal changes in morphology (34,35), force (36,37), or near-field coupling (38)(39)(40), using an atomic force microscope cantilever as local probe, has been reported for point-by-point IR measurements. Noncontact optical photothermal microscopy is more recent (41)(42)(43)(44)(45)(46)(47)(48)(49)(50) and typically utilizes a local IR illumination coincident with a highly focused visible probe beam to measure local refractive index change by beam scattered out of the angular acceptance of the objective lens. This method has been applied to chemical imaging of tissue and live cells (44,46,47), bacteria (48), and pharmaceutical tablets (49).…”

Section: Significancementioning

confidence: 99%

“…Noncontact optical photothermal microscopy is more recent (41)(42)(43)(44)(45)(46)(47)(48)(49)(50) and typically utilizes a local IR illumination coincident with a highly focused visible probe beam to measure local refractive index change by beam scattered out of the angular acceptance of the objective lens. This method has been applied to chemical imaging of tissue and live cells (44,46,47), bacteria (48), and pharmaceutical tablets (49). These point-scanning approaches, just as for point scanning FT-IR spectroscopy, involve long scan times (51).…”

Section: Significancementioning

confidence: 99%

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All-digital histopathology by infrared-optical hybrid microscopy

Schnell

Mittal

Falahkheirkhah

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

118

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Optical microscopy for biomedical samples requires expertise in staining to visualize structure and composition. Midinfrared (mid-IR) spectroscopic imaging offers label-free molecular recording and virtual staining by probing fundamental vibrational modes of molecular components. This quantitative signal can be combined with machine learning to enable microscopy in diverse fields from cancer diagnoses to forensics. However, absorption of IR light by common optical imaging components makes mid-IR light incompatible with modern optical microscopy and almost all biomedical research and clinical workflows. Here we conceptualize an IR-optical hybrid (IR-OH) approach that sensitively measures molecular composition based on an optical microscope with wide-field interferometric detection of absorption-induced sample expansion. We demonstrate that IR-OH exceeds state-of-the-art IR microscopy in coverage (10-fold), spatial resolution (fourfold), and spectral consistency (by mitigating the effects of scattering). The combined impact of these advances allows full slide infrared absorption images of unstained breast tissue sections on a visible microscope platform. We further show that automated histopathologic segmentation and generation of computationally stained (stainless) images is possible, resolving morphological features in both color and spatial detail comparable to current pathology protocols but without stains or human interpretation. IR-OH is compatible with clinical and research pathology practice and could make for a cost-effective alternative to conventional stain-based protocols for stainless, all-digital pathology.

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Section: Significancementioning

confidence: 99%

Section: Significancementioning

confidence: 99%

All-digital histopathology by infrared-optical hybrid microscopy

Schnell

Mittal

Falahkheirkhah

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

118

View full text Add to dashboard Cite

show abstract

“…IR imaging is limited by the water absorption and low spatial resolution, even though recent concepts have improved on the spatial resolution. 2 As an alternative, coherent Raman scattering (CRS) imaging has been used, but fastest implementations are based on imaging the distribution of a single vibrational Raman resonance only, instead of the full or a significant part of the vibrational spectrum, thereby significantly reducing the molecular information. This is due to the fact that changes in the spectral shape or shifts in the peak position cannot be extracted from few images but require spectral information.…”

Section: All Article Content Except Where Otherwise Noted Is Licensmentioning

confidence: 99%

Invited Article: Comparison of hyperspectral coherent Raman scattering microscopies for biomedical applications

et al. 2018

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Raman scattering based imaging represents a very powerful optical tool for biomedical diagnostics. Different Raman signatures obtained by distinct tissue structures and disease induced changes provoke sophisticated analysis of the hyperspectral Raman datasets. While the analysis of linear Raman spectroscopic tissue data is quite established, the evaluation of hyperspectral nonlinear Raman data has not yet been evaluated in great detail. The two most common nonlinear Raman methods are CARS (coherent anti-Stokes Raman scattering) and SRS (stimulated Raman scattering) spectroscopy. Specifically the linear concentration dependence of SRS as compared to the quadratic dependence of CARS has fostered the application of SRS tissue imaging. Here, we applied spectral processing to hyperspectral SRS and CARS data for tissue characterization. We could demonstrate for the first time that similar cluster distributions can be obtained for multispectral CARS and SRS data but that clustering is based on different spectral features due to interference effects in CARS and the different concentration dependence of CARS and SRS. It is shown that a direct combination of CARS and SRS data does not improve the clustering results.

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“…The thermal effect of laser irradiation is so small that could not damage or destroy the irradiated tissue. Such fiber lasers are widely used in therapy [41][42][43], diagnostics [44][45][46], cosmetics [40,42], and research [47,48]. The second group refers to the thermal response and members of this group are often called ablative fiber lasers.…”

Section: Ablative Versus Non-ablative Fiber Lasersmentioning

confidence: 99%

Fiber Lasers and Their Medical Applications

Gursel¹

2018

Optical Amplifiers - A Few Different Dimensions

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Advancing of photonics, aided with fruitful and abundant experimental and theoretical studies, over the last four decades has brought about the invention of a large variety of lasers. Among them one of the most popular types is a fiber laser, which is a variation of the standard solid-state laser, with the medium being a clad fiber waveguide structure and different dopants inside core serve as a gain media. They were derived from erbiumdoped fiber amplifiers, which are still important component for telecommunications. Since discovery, fiber laser has become a natural choice for many uses, primarily because of the physical characteristics of fiber waveguide structure. Their rapid progress may show how excellent they really are. Although fiber lasers are today widely used in various research and industrial areas, one of the most meaningful applications of fiber laser technology has been through its use in medicine. A wide variety of wavelengths generated by fiber lasers as well as the diversity of physical mechanisms employed in pulse generation also additionally underpins the flexibility of fiber laser technology. This study is devoted to background technology of fiber lasers in the light of medical applications. Basic physics and theories of optical fibers and their important properties are introduced.

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Bond-Selective Imaging of Cells by Mid-Infrared Photothermal Microscopy in High Wavenumber Region

Cited by 54 publications

References 48 publications

All-digital histopathology by infrared-optical hybrid microscopy

All-digital histopathology by infrared-optical hybrid microscopy

Invited Article: Comparison of hyperspectral coherent Raman scattering microscopies for biomedical applications

Fiber Lasers and Their Medical Applications

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