Label-free inertial-ferrohydrodynamic cell separation with high throughput and resolution

Abstract

Rapid and label-free separation of target cells in biological samples provided unique opportunity for disease diagnostics and treatment. However, even with advanced technologies for cell separation, the limiting throughput, high...

Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells

“…Prostate tumor cells (DU145) were effectively isolated from peripheral blood mononuclear cells at a recovery rate of 76.2 ± 5.9%. Furthermore, Liu et al designed a label-free inertial-ferrohydrodynamic CTC-capturing microfluidic device [158] (Figure 6a). The technique enabled the high-throughput, high-resolution isolation of CTCs.…”

Tuning particle inertial separation in sinusoidal channels by embedding periodic obstacle microstructures

“…Besides, the recovery ratio of cancer cells was 55.95% and 40.89% for the samples with initial cancer cell ratios of 0.01% and 0.001%, respectively. The separation performance of the developed cascaded sinusoidal device is superior compared to the most reported single devices 23,37,56,69 and comparable to the state-of-the-art integrated microfluidic systems [70][71][72][73][74] on the aspects of enrichment and final purity, Table S1. † It should be noted that the cell recovery is generally compromised in the cascaded devices because of the successive loss during processing.…”

Basic Principles and Recent Advances in Magnetic Cell Separation

“…As previously seen, several studies show the value of integrating particular geometries in the channel to combine hydrodynamic effects with separation based on magnetophoretic properties (e.g., grooves [175,176], channel enlargement [194], sheath flow [152,170], etc.). Other passive methods can also be integrated to further increase separation efficiency and throughput.…”

“…Similarly, Dumas Bouchiat et al have developed an array of oppositely magnetized micromagnets capable of producing magnetic field gradients as high as 10 5 T/m, obtained by thermomagnetic patterning of a flat NdFeB hard magnetic film [167], which can be integrated at the bottom of a microfluidic channel [44,168] (Figure 11D). Creating higher, asymmetric [169] gradient magnetic fields and forces, locating the maximum flux density at the center of the channel [170,171] (Figure 11E) or at multiple spots [172], are different options provided by adequate arrangement of several magnets, including the quadrupole magnet arrangement mentioned earlier in Section 4.1.…”

“…Six magnets, placed above and below the channel, create a maximum flux density at the center of the channel. Reprinted from [170] with the permission of Royal Society of Chemistry; permission conveyed through Copyright Clearance Center, Inc.…”